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Cloning and functional identification of apple LATERAL ORGAN BOUNDARY DOMAIN 3 (LBD3) transcription factor in the regulation of drought and salt stress

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Abstract

Main conclusion

Overexpression of MdLBD3 in Arabidopsis reduced sensitivity to salt and drought stresses and was instrumental in promoting early flowering.

Abstract

Salt and drought stresses have serious effects on plant growth. LATERAL ORGAN BOUNDARY DOMAIN (LBD) proteins are a plant-specific transcription factors (TFs) family and play important roles in plants in resisting to abiotic stress. However, about the function of LBDs in apple and other woody plants is little known. In this study, protein sequences of the LBD family TFs in apples were identified which contained conserved LOB domains. The qRT-PCR analysis showed that the MdLBD3 gene was widely expressed in various tissues and organs. The subcellular localization assay showed that the MdLBD3 protein was localized in the nucleus. Ectopic expression of MdLBD3 in Arabidopsis positively regulated its salt and drought resistance, and promoted early flowering. Collectively, these results showed that MdLBD3 improved the abiotic stress resistance, plant growth and development. Overall, this study provided a new gene for breeding that can increase the abiotic stress tolerance in apple.

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Data availability

The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

LBD:

LATERAL ORGAN BOUNDARIES (LOB) DOMAIN

TF:

Transcription factor

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Funding

This work was supported by the National Natural Science Foundation of China (32272683), the Shandong Province Key R&D Program of China (2022TZXD008-02), and the China Agriculture Research System of MOF and MARA (CARS-27).

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Authors and Affiliations

  1. Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China

    Yaqi Liu, Haofeng Liu, Tingting Zhang, Xiaowen Li, Ranxin Liu, Chang Li, Chunxiang You & Xiao-Fei Wang

  2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province, Hebei Agricultural University, Baoding, Hebei, China

    Xiu-Hong An & Yi Tian

Authors
  1. Yaqi Liu
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  2. Xiu-Hong An
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  3. Haofeng Liu
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  4. Tingting Zhang
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  5. Xiaowen Li
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  9. Chunxiang You
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  10. Xiao-Fei Wang
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Contributions

YL: conducted experiments and wrote original draft. XHA, HL, and TZ: designed experiments. XL, RL, CL, and YT: collated data. CY and XFW: revised the manuscript.

Corresponding authors

Correspondence to Chunxiang You or Xiao-Fei Wang.

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Liu, Y., An, XH., Liu, H. et al. Cloning and functional identification of apple LATERAL ORGAN BOUNDARY DOMAIN 3 (LBD3) transcription factor in the regulation of drought and salt stress. Planta 259, 125 (2024). https://doi.org/10.1007/s00425-024-04373-7

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