Abstract
Main conclusion
MdPRX34L enhanced resistance to Botryosphaeria dothidea by increasing salicylic acid (SA) and abscisic acid (ABA) content as well as the expression of related defense genes.
Abstract
The class III peroxidase (PRX) multigene family is involved in complex biological processes. However, the molecular mechanism of PRXs in the pathogen defense of plants against Botryosphaeria dothidea (B. dothidea) remains unclear. Here, we cloned the PRX gene MdPRX34L, which was identified as a positive regulator of the defense response to B. dothidea, from the apple cultivar 'Royal Gala.' Overexpression of MdPRX34L in apple calli decreased sensitivity to salicylic acid (SA) and abscisic acid（ABA). Subsequently, overexpression of MdPRX34L in apple calli increased resistance to B. dothidea infection. In addition, SA contents and the expression levels of genes related to SA synthesis and signaling in apple calli overexpressing MdPRX34L were higher than those in the control after inoculation, suggesting that MdPRX34L enhances resistance to B. dothidea via the SA pathway. Interestingly, infections in apple calli by B. dothidea caused an increase in endogenous levels of ABA followed by induction of ABA-related genes expression. These findings suggest a potential mechanism by which MdPRX34L enhances plant–pathogen defense against B. dothidea by regulating the SA and ABA pathways.
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All data generated or analyzed during this study are provided in this published article and its supplementary data files.
Abbreviations
- MdPRX34L-OE:
-
Overexpression of MdPRX34L
- PRX:
-
Peroxidase
- ROS:
-
Reactive oxygen species
- OFR:
-
Oxygen free radical
- SOD:
-
Superoxide dismutase
- SA:
-
Salicylic acid
- ABA:
-
Abscisic acid
- SAR:
-
Systemic acquired resistance
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Acknowledgements
This work was supported by grants from the National Key Research and Development Program of China (2022YFD2100102), National Natural Science Foundation of China (32122080, 31972375), and Natural Science Foundation of Shandong Province (ZR2020YQ25).
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DGH. conceived and designed the study. YWZ, WKL, CKW, and QS performed the experiments. WYW, XYH, and YX analyzed the data. DGH and YWZ wrote the paper. All authors discussed the results and commented on the manuscript.
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Zhao, YW., Li, WK., Wang, CK. et al. MdPRX34L, a class III peroxidase gene, activates the immune response in apple to the fungal pathogen Botryosphaeria dothidea. Planta 259, 86 (2024). https://doi.org/10.1007/s00425-024-04355-9
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DOI: https://doi.org/10.1007/s00425-024-04355-9