Abstract
Main conclusion
In the anhydrobiotic midge Polypedilum vanderplanki , LEA family proteins are likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration.
The larvae of the anhydrobiotic midge, P. vanderplanki, which can tolerate almost complete desiccation, accumulate late embryogenesis abundant (LEA) proteins in response to drying. Using complete genome data of the midge, we have identified 27 PvLea1-like genes based on the similarity to previously characterized PvLea1 gene belonging to group 3 LEA proteins. Generally, group 3 LEA proteins are characterized by several repetitions of an 11-mer motif. However, some PvLea genes lack the canonical motif in their sequences. We performed the detailed characterization of all 27 PvLea genes in terms of biochemical and biophysical properties and conserved motifs. The motif analysis among their amino acid sequences revealed that all 27 PvLEA proteins have at least one of two types of motifs (motif 1: G AKDTTKEKLGE AKDATAEKLG or motif 2: KD ILExAKDKLxD AKDAVKEKL), indicating the presence of at least two repeated 11-mer LEA motifs. Most of PvLEA proteins were localized to the cytosol. We also performed quantitative real-time PCR of all 27 PvLea genes in detail during the process of desiccation and rehydration. The expression of these genes was upregulated at the beginning of dehydration, the latter phase of the desiccation process and on rehydration process. These data suggested that each LEA protein is likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration.
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Abbreviations
- CHO:
-
Chinese hamster ovary
- ER:
-
Endoplasmic reticulum
- LEA:
-
Late embryogenesis abundant
- GFP:
-
Green fluorescent protein
- GRAVY:
-
Grand average of hydropathy
- qRT-PCR:
-
Quantitative real-time PCR
- pI:
-
Isoelectric point
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Acknowledgments
We extend our gratitude to the Federal Ministry of Environment of Nigeria for permitting research on P. vanderplanki. We thank Y. Saito, T. Shiratori, Y. Kikuzato and A. Saito for technical assistance. This work was supported in part by Grants-in-Aids from MEXT/JSPS KAKENHI (Grant Numbers 12JJ10847 for RH; 23780055 for RC; 25128712, 25128714 and 60414900 for TK), Japan; subsidy of the Russian Government to support the Program of competitive growth of Kazan Federal University among world class academic centers and universities and Russian Foundation for Basic Research No 14-04-01657_A and Ministry of Education of Russia Subsidy N 14.584.21.0002 for international cooperation.
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Special topic: Desiccation Biology.
Guest editors: Olivier Leprince and Julia Buitink.
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Hatanaka, R., Gusev, O., Cornette, R. et al. Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki . Planta 242, 451–459 (2015). https://doi.org/10.1007/s00425-015-2284-6
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DOI: https://doi.org/10.1007/s00425-015-2284-6