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Choline induces opposite changes in pyramidal neuron excitability and synaptic transmission through a nicotinic receptor-independent process in hippocampal slices

  • Neuroscience
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Abstract

Choline is present at cholinergic synapses as a product of acetylcholine degradation. In addition, it is considered a selective agonist for α5 and α7 nicotinic acetylcholine receptors (nAChRs). In this study, we determined how choline affects action potentials and excitatory synaptic transmission using extracellular and intracellular recording techniques in CA1 area of hippocampal slices obtained from both mice and rats. Choline caused a reversible depression of evoked field excitatory postsynaptic potentials (fEPSPs) in a concentration-dependent manner that was not affected by α7 nAChR antagonists. Moreover, this choline-induced effect was not mimicked by either selective agonists or allosteric modulators of α7 nAChRs. Additionally, this choline-mediated effect was not prevented by either selective antagonists of GABA receptors or hemicholinium, a choline uptake inhibitor. The paired pulse facilitation paradigm, which detects whether a substance affects presynaptic release of glutamate, was not modified by choline. On the other hand, choline induced a robust increase of population spike evoked by orthodromic stimulation but did not modify that evoked by antidromic stimulation. We also found that choline impaired recurrent inhibition recorded in the pyramidal cell layer through a mechanism independent of α7 nAChR activation. These choline-mediated effects on fEPSP and population spike observed in rat slices were completely reproduced in slices obtained from α7 nAChR knockout mice, which reinforces our conclusion that choline modulates synaptic transmission and neuronal excitability by a mechanism independent of nicotinic receptor activation.

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Acknowledgements

This work was supported by “Instituto de Salud Carlos III” (Grant PI080227 to J.M.H-G and Grant PI081067 to J.M.S.). E.A. is a fellow of “Fundación Teófilo Hernando”. The authors gratefully acknowledge the technical assistance of José Barbado. We also thank Fundación Teófilo Hernando for continued support.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutic, University Autónoma de Madrid, Av. Arzobispo Morcillo 4, 28029, Madrid, Spain

    E. Albiñana, J.G. Luengo, A.M. Baraibar, L. Gandía & J.M. Hernández-Guijo

  2. Instituto Teófilo Hernando, Facultad de Medicina, University Autónoma de Madrid, 28029, Madrid, Spain

    E. Albiñana, J.G. Luengo, A.M. Baraibar, L. Gandía & J.M. Hernández-Guijo

  3. Servicio de Neurología Experimental, Hospital Universitario Ramón y Cajal, IRYCIS, 28034, Madrid, Spain

    M.D. Muñoz

  4. Servicio de Neurobiología-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, 28034, Madrid, Spain

    J.M. Solís

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  1. E. Albiñana
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Correspondence to J.M. Hernández-Guijo.

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Albiñana, E., Luengo, J., Baraibar, A. et al. Choline induces opposite changes in pyramidal neuron excitability and synaptic transmission through a nicotinic receptor-independent process in hippocampal slices. Pflugers Arch - Eur J Physiol 469, 779–795 (2017). https://doi.org/10.1007/s00424-017-1939-5

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