Abstract
Purpose
To investigate acute changes in biochemical markers of bone and cartilage turnover in response to moderate intensity exercise with and without joint impact in healthy human subjects.
Methods
A randomized, cross-over, exploratory, clinical study was conducted. Twenty healthy subjects with no history of joint trauma completed 30 min interventions of standardized moderate intensity cycling and running as well as a resting intervention 1 week apart. Blood samples were taken immediately before, four times after exercise and again the next day. Urine was sampled, before, after and the next day. On the day of rest, samples were taken at timepoints similar to the days of exercise. Markers of type I (CTX-I), II (C2M, CTX-II) and VI (C6M) collagen degradation, cartilage oligomeric matrix protein (COMP) and procollagen C-2 (PRO-C2) was measured. Trial registration number: NCT04542655, 02 September 2020, retrospectively registered.
Results
CTX-I was different from cycling (4.2%, 95%CI: 0.4–8.0%, p = 0.03) and resting (6.8%, 95%CI: 2.9–10.7%, p = 0.001) after running and the mean change in COMP was different from cycling (10.3%, 95%CI: 1.1–19.5%, p = 0.03), but not from resting (8.6%, 95%CI: − 0.7–17.8%, p = 0.07) after running. Overall, changes in other biomarkers were not different between interventions.
Conclusion
In this exploratory study, running, but not cycling, at a moderate intensity and duration induced acute changes in biomarkers of bone and cartilage extra-cellular matrix turnover.
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Abbreviations
- BMI:
-
Body mass index
- CV:
-
Coefficient of variation
- COMP:
-
Cartilage oligomeric matrix protein
- CTX-I:
-
C-terminal telopeptide of type I collagen
- CTX-II:
-
C-terminal telopeptide of type II collagen
- C2M:
-
MMP-generated fragment of type II collagen
- C6M:
-
MMP-generated fragment of type VI collagen
- ELISA:
-
Enzyme-linked immunosorbent assays
- ICC:
-
Intraclass correlation
- KOOS:
-
Knee injury and osteoarthritis outcome score
- LLOQ:
-
Lower limit og quantification
- MMP:
-
Matrix metalloproteinase
- MRI:
-
Magnetic resonance imaging
- OA:
-
Osteoarthritis
- PRO-C2:
-
Procollagen type II
- R:
-
Range
- SEM:
-
Standard error of the mean
- ULOQ:
-
Upper limit of quantification
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Acknowledgements
The authors thank Heidi Rønberg for lab assistance, Melanie Kurell and Sarah á Rogvi for clinical assistance and Dr. Kamilla Mundbjerg for technical support.
Funding
This study was funded by Nordic Bioscience Clinical Development and the Danish Research Fund.
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JJB, ARB, JRA and HBN designed the protocol. JJB and MB acquired the clinical data. JJB, ARB, and IB conducted the data analysis. JJB, ARB, MK, CT and ALM interpreted the data. JJB drafted the manuscript. Critical revision and approval of the final manuscript was done by all of the authors.
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JJB received funding from Nordic Bioscience Clinical Development and the Danish Research Fund. IB and CT are full-time employees of Nordic Bioscience. JRA, MK and ARB are full-time employees and shareholders of Nordic Bioscience. MB and ALM have no conflict of interest.
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Bjerre-Bastos, J.J., Nielsen, H.B., Andersen, J.R. et al. A biomarker perspective on the acute effect of exercise with and without impact on joint tissue turnover: an exploratory randomized cross-over study. Eur J Appl Physiol 121, 2799–2809 (2021). https://doi.org/10.1007/s00421-021-04751-z
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DOI: https://doi.org/10.1007/s00421-021-04751-z