Abstract
Purpose
Blood rheology is a key determinant of blood flow and tissue perfusion. There are still large discrepancies regarding the effects of an acute running exercise on blood rheological properties and red blood cell (RBC) physiology. We investigated the effect of a 10 km running trial on markers of blood rheology and RBC physiology in endurance trained athletes.
Methods
Blood was sampled before and after the exercise to measure lactate and glucose, hematological and hemorheological parameters (blood viscosity, RBC deformability, and aggregation), eryptosis markers (phosphatidylserine and CD47 exposure, RBC reactive oxygen species), RBC-derived microparticles (RBC-MPs), and RBC electrophysiological activity. Weight was measured before and after exercise. Peripheral oxygen saturation and heart rate were monitored before and during the trial.
Results
Blood lactate and glucose levels increased after exercise and subjects significantly lost weight. All athletes experienced a significant fall in oxygen saturation. Mean corpuscular volume (MCV) was increased from 95.1 ± 3.2 to 96.0 ± 3.3 and mean corpuscular hemoglobin concentration (MCHC) decreased after exercise suggesting a slight RBC rehydration. Exercise increased RBC deformability from 0.344 ± 0.04 to 0.378 ± 0.07, decreased RBC aggregates strength and blood viscosity, while hematocrit (Hct) remained unaffected. While RBC electrophysiological recording suggested a modulation in RBC calcium content and/or chloride conductance, eryptosis markers and RBC-MPs were not modified by the exercise.
Conclusion
A 10 km acute running exercise had no effect on RBC senescence and membrane blebbing. In contrast, this exercise increased RBC deformability, probably through rehydration process which resulted in a decrease in blood viscosity.
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Abbreviations
- Ca2+ :
-
Calcium
- CCCP:
-
Carbonyl cyanide m-chlorophenylhydrazone
- EI:
-
Elongation index
- Hb:
-
Hemoglobin
- Hct:
-
Hematocrit
- HR:
-
Heart rate
- Hypermax :
-
Red blood cell maximum hyperpolarization
- MAS:
-
Maximal aerobic speed
- MCH:
-
Mean corpuscular hemoglobin
- MCHC:
-
Mean corpuscular hemoglobin concentration
- MCV:
-
Mean corpuscular volume
- MFI:
-
Median fluorescence intensity
- MPs:
-
Microparticles
- NS:
-
Not significant
- PLA:
-
Platelets
- PS:
-
Phosphatidylserine
- RBC:
-
Red blood cells
- ROS:
-
Reactive oxygen species
- RT:
-
Room temperature
- SPO2 :
-
Peripheral oxygen saturation
- S/V:
-
Surface to volume ratio
- Vm :
-
Membrane potential
- VO2 max :
-
Maximal oxygen consumption
- WBC:
-
White blood cells
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All the authors approved the final version of the manuscript. EN, PC, DM, SE, MR, SS performed research, analyzed the data, and wrote the manuscript. ES, JH, and MG performed research. CR, PJ, AC, and AS edited the manuscript.
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Nader, E., Monedero, D., Robert, M. et al. Impact of a 10 km running trial on eryptosis, red blood cell rheology, and electrophysiology in endurance trained athletes: a pilot study. Eur J Appl Physiol 120, 255–266 (2020). https://doi.org/10.1007/s00421-019-04271-x
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DOI: https://doi.org/10.1007/s00421-019-04271-x