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No evidence of cardiovascular toxicity in workers exposed below 5 ppm carbon disulfide

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Abstract

Purpose

Carbon disulfide (CS2), used in the viscose process, is well known for having multiple health effects, including on the cardiovascular system, in workers with long-term exposure higher than 10 ppm. The mechanisms of those effects are, however, not precisely defined, and it remains uncertain whether cardiovascular toxicity may occur at exposure levels lower than 10 ppm. Therefore, the purpose of this study is to explore the health impact of low CS2 exposure levels using an array of preclinical biomarkers of cardiovascular risk.

Methods

Exposure intensity was determined by measuring urinary 2-thiothiazolidine-4-carboxylic acid (TTCA) in 117 workers from two plants using the viscose process, sampled in multiples phases (2003, 2006 and 2013). A cumulative exposure index (CEI) and a recent exposure index (REI) were calculated for each worker, and shiftwork was documented to account for potential confounding. Cardiovascular parameters included blood pressure, total, LDL and HDL cholesterol, triglycerides, C-reactive protein dosed in serum with high sensitivity (HsCRP), N-terminal pro-brain natriuretic peptide, and albuminuria/creatininuria ratio (UACR). Potential biological confounders were fasting blood glucose and serum creatinine. Bivariate and multivariate regression analyses were used to trace relationships between cardiovascular risk biomarkers and other variables, including CEI, REI and shiftwork duration.

Results

Median REI and CEI were 0.05 mg TTCA/g creat and 21.5 mg TTCA/g creat*months, respectively. While expected associations, such as between HsCRP and LDL Cholesterol, were found, significant associations between cardiovascular risk markers and CS2 exposure indexes (CEI or REI) were not detected. Shiftwork duration was positively associated with UACR in workers with elevated fasting blood glucose.

Conclusion

In practice, when CS2 exposure levels are kept below 5 ppm (TTCA < 2.2 mg/g creat), it does not appear useful to perform a systematic monitoring of total serum cholesterol or its subfractions, or of the new biomarkers of cardiovascular risk (NTproBNP, HsCRP, UACR) investigated in the present study. It appears important to carefully monitor the existence of diabetes that may justify avoiding shiftwork.

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Abbreviations

HsCRP:

CRP measured by highly sensitive assay

NT-proBNP:

N-terminal pro-brain natriuretic peptide

Lp(a):

Lipoprotein (a)

CV:

Cardiovascular

UACR:

Urinary albumin/creatinine ratio

MI:

Myocardial infarction

AF:

Atrial fibrillation

BMI:

Body mass index

CEI/REI:

Cumulative/recent exposure index

CS2 :

Carbon disulfide

Creat:

Creatinine

TTCA:

Urinary 2-thiothiazolidine-4-carboxylic acid

OEL:

Occupational exposure limit

BP:

Blood pressure

FBG:

Fasting blood glucose

SW:

Shiftwork duration

Pck-y:

Smoking in pack-years

LOD:

Limit Of Detection

ECG:

Electrocardiogram

TLV:

Threshold Limit Value

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Authors and Affiliations

  1. Centre inter-entreprises et artisanal de Médecine du travail, 45 rue des Vinaigriers, 75010, Paris, France

    Jérôme Domergue

  2. Louvain centre for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Brussels, Belgium

    Dominique Lison & Vincent Haufroid

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  1. Jérôme Domergue
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  2. Dominique Lison
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  3. Vincent Haufroid
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Correspondence to Jérôme Domergue.

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

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Domergue, J., Lison, D. & Haufroid, V. No evidence of cardiovascular toxicity in workers exposed below 5 ppm carbon disulfide. Int Arch Occup Environ Health 89, 835–845 (2016). https://doi.org/10.1007/s00420-016-1122-x

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  • DOI: https://doi.org/10.1007/s00420-016-1122-x

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