Abstract
The mystery of how human DNA is compactly packaged into a nucleus—a space a hundred thousand times smaller—while still allowing for the regulation of gene function, has long been one of the greatest enigmas in cell biology. This puzzle is gradually being solved, thanks in part to the advent of new technologies. Among these, innovative genome-labeling techniques combined with high-resolution imaging methods have been pivotal. These methods facilitate the visualization of DNA within intact nuclei and have significantly contributed to our current understanding of genome organization. This review will explore various labeling and imaging approaches that are revolutionizing our understanding of the three-dimensional organization of the genome, shedding light on the relationship between its structure and function.
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Introduction
The discovery of the double helix structure of the DNA by James Watson and Francis Crick in 1953 (Watson and Crick 1953), building on critical X-ray crystallography work by Rosalind Franklin (Franklin and Gosling 1953), was a pivotal moment in molecular biology, as it provided the fundamental understanding of how DNA encodes genetic information and how it is replicated. The discovery of the Feulgen stain came nearly three decades prior to this breakthrough after the pioneering discovery of Robert Feulgen in 1924, a time when the molecular nature of DNA was unknown. The Feulgen stain was a crucial advancement in histology, enabling the visualization of DNA within cells and the observation of nuclear changes during cell division and differentiation (Chieco and Derenzini 1999; Mello and Vidal 2017). This method heralded a new era in the visualization and quantification of DNA.
Our contemporary understanding of human DNA—consisting of 46 chromosomes that all together measure 2 m in length, intricately packed and organized within the cell nucleus as chromatin—has been profoundly shaped by years of innovation in DNA labeling technologies (Lakadamyali and Cosma 2020). These advancements have progressively enhanced our ability to visualize DNA with remarkable specificity and resolution at the cellular level. We now understand that DNA is intricately folded inside the three-dimensional (3D) space of the nucleus to enable communication between distal genomic sequences, including between enhancers and promoters (Misteli 2020). DNA is organized at multiple length scales, beginning with the nucleosome, in which 146 base pairs of DNA wrap around an octamer of histone proteins (Luger et al. 1997). Repeating units of nucleosomes form the beads-on-a-string fiber, also known as the 10 nm fiber (Olins and Olins 1974). The nucleosomes coalesce into irregular domains known as nucleosome clutches at tens of nanometer length scale (Ricci et al. 2015), followed by chromatin nanodomains in the hundreds of nanometer range (Szabo et al. 2020; Nozaki et al. 2017), both of which depend on nucleosome-nucleosome interactions and are disrupted by histone hyperacetylation (Otterstrom et al. 2019; Ricci et al. 2015; Szabo et al. 2020). Additional levels of organization involve topologically associating domains (TADs) (which are made up of multiple chromatin nanodomains) (Nora et al. 2012; Szabo et al. 2020) and A and B compartments (or euchromatin and heterochromatin) (Lieberman-Aiden et al. 2009), as well as chromosome territories (Cremer and Cremer 2010) extending to even larger length scales. This multilevel organization is established and maintained by various mechanisms, including loop extrusion by architectural proteins such as cohesion and CTCF (de Wit and Nora 2023), chromatin anchoring to the nuclear lamina (van Steensel and Belmont 2017), post-translational modifications of nucleosomes that are recognized and bound by various proteins such as the polycomb group proteins (Millán-Zambrano et al. 2022), and phase separation of histones with each other or with other nuclear bodies such as nuclear speckles (Strom and Brangwynne 2019).
This review will focus on the advances in labeling and microscopy methods that have been instrumental in deepening our understanding of the 3D genome organization, charting a journey from the foundational Feulgen stain to the sophisticated imaging techniques of today.
DNA dyes and light microscopy
Histological techniques, such as the Feulgen stain (Fig. 1A), have evolved significantly with the advent of fluorescent DNA dyes (Gomes et al. 2018; Suzuki et al. 1997). These dyes emit specific wavelengths of light when excited by a wavelength of light that they absorb, providing exquisite contrast when visualizing DNA. Fluorescent dyes are further attractive as they simplify the visualization of DNA without necessitating complex sample preparation methods, such as transfection with exogenous fluorescent proteins. These fluorescent dyes include those that bind to the DNA major or minor groove such as DAPI (Fig. 1B), Hoechst dyes, SYTO dyes (e.g., various SYTOX variants) and PicoGreen. Additionally, high affinity cyanine dyes that intercalate into DNA such as YOYO-1 and TOTO-1 can be used for staining nucleic acids in fluorescence microscopy. Many of these dyes, including, YOYO-1, SYTO, and PicoGreen have fluorogenic properties in which a large fluorescence enhancement is achieved upon DNA binding, greatly minimizing fluorescence background.
While certain DNA dyes are restricted to fixed cells, many, including Hoechst, SYTO dyes, YOYO, and TOTO series are compatible with living cells (Martin et al. 2005). This enables studying real time DNA dynamics and organization during key cellular processes, such as cell division and differentiation. A recent, notable advancement in this domain is the SiR-DNA dye, which is based on the attachment of carboxylated silicon-rhodamine (SiR) dye to the bisbenzimide core of Hoechst 33,342. This attachment targets the SiR dye to DNA (Lukinavičius et al. 2015). The SiR derivatives are advantageous over other fluorophores as they are excited by far-red light, which induces less phototoxicity in live cell imaging compared with blue or green light illumination. Additionally, the SiR dyes are compatible with super-resolution light microscopy (see below for more details), making the SiR-DNA an attractive DNA dye for live cell and super-resolution imaging although some reports suggest that this stain can induce DNA damage response and impair cell cycle (Sen et al. 2018).
DNA dyes, such as DAPI and Hoechst, have become integral in cellular biology, frequently used as nuclear markers in both live and fixed cell studies. Their applications range from monitoring cell division during developmental stages and analyzing chromosome segregation anomalies to assessing chromatin condensation in varying cell types or during differentiation offering critical insights into cellular processes and genomic organization.
Fluorescence in situ hybridization, oligopaints, and high throughput genome imaging
DNA dyes offer many advantages such as ease of use and live cell compatibility. However, these dyes mostly label the entire DNA within the nucleus and lack sequence specificity, which is crucial for understanding the nonrandom organization and folding patterns of chromosomes and genomic domains. DNA fluorescence in situ hybridization or DNA-FISH is a decades old technique (Bauman et al. 1980; Gall and Pardue 1969) that enables visualizing specific genomic sequences and their locations within the nucleus. This technique utilizes fluorescently labeled FISH probes that hybridize with target sequences. Traditionally, these probes have been derived from genomic sequences cloned into vectors such as bacterial artificial chromosome (BAC) plasmids or from custom-synthesized oligonucleotides (oligos) (Finn et al. 2022). Oligo probes offer advantages such as precise targeting and efficient nuclear diffusion due to their shorter length. However, the high costs associated with oligo synthesis limited their use until the development of “oligopaints,” which are more cost-effective at least at small scale (Beliveau et al. 2012) (Fig. 2A). This approach utilizes oligo libraries as a renewable probe source, amplified with fluorophore-conjugated polymerase chain reaction (PCR) primers to create efficient, single-stranded, and strand-specific probes. Oligopaints can visualize genomic regions ranging from a few kilobases (Mateo et al. 2019) to entire chromosomes (Nir et al. 2018; Wang et al. 2016). The use of DNA-FISH and oligopaints have significantly advanced our understanding of the organizational principles of the genome. For instance, they have demonstrated that developmentally important genes are located at the nuclear periphery when silenced and move to the interior when active (Chambeyron and Bickmore 2004). Since DNA-FISH and oligopaints require harsh denaturing treatments, concerns have been raised about the preservation of genomic sequences. This concern is partially mitigated by the bulk level agreement between DNA-FISH and Hi-C (Bintu et al. 2018), the latter of which does not require denaturation. Nonetheless, recent advances that use exonuclease digestion rather than heat denaturation to generate single stranded DNA for efficient probe labeling, including resolution after single-strand exonuclease reaction (RASER)-FISH (Brown et al. 2022), address this potential concern.
Until recently, one major limitation of DNA-FISH and oligopaints was their low throughput. Due to the limited number of spectrally distinct fluorophores and imaging channels, only three to four loci could be visualized in a handful of cells at a time. This limitation has now been overcome with the next generation labeling and imaging methods that drastically improve the throughput of DNA-FISH and oligopaints. Advances in automated image acquisition and automated image analysis have been leveraged to introduce high-throughput FISH (HiFISH) (Finn et al. 2022; Finn and Misteli 2021). HiFISH was used to image up to three loci in an automated and high-throughput manner in hundreds of cells and this imaging could be repeated for hundreds of loci pairs. Application of HiFISH to hundreds of genomic loci revealed a high level of heterogeneity in the relative positioning of these loci within the nucleus (Finn et al. 2019). High throughput genomic imaging technologies has also made it possible to carry out genomewide imaging-based siRNA screens. High-throughput DNA or RNA labeling with optimized oligopaints (HiDRO) is an automated imaging pipeline that enables chromatin interaction measurements in single cells across thousands of samples (Park et al. 2023). Combined with siRNA screens, HiDRO could identify new regulators of genome folding (Park et al. 2023).
The spectral limitation has been overcome by sequential imaging in methods such as Hi-M, where multiple DNA loci are labeled and visualized sequentially in conjunction with RNA transcripts (Cardozo Gizzi et al. 2019). Surprisingly, Hi-M showed that the physical proximity of distal regulatory elements and promoters is not strongly correlated with transcriptional states in a Drosophila model system (Espinola et al. 2021). Sequential labeling and imaging also enable tracing the 3D path of genomic DNA, unveiling how different genomic sequences fold in the 3D space of the nucleus. This approach was used to visualize and trace individual TADs within entire chromosomes to show that TADs are organized in two polarized compartments in individual chromosomes (Wang et al. 2016). Optical reconstruction of chromatin architecture (ORCA) is a microscopy approach that uses sequential hybridization and imaging to trace the 3D DNA path with a genomic resolution as fine as 2 kb and a throughput of tens of thousands of cells (Mateo et al. 2019) (Fig. 2B). ORCA is revealing that several loci fold into a complex pattern of stacked, multiloop configurations that bridge enhancers and promoters to regulate transcription (Chen et al. 2023; Hafner et al. 2023).
In sequential imaging methods, such as Hi-M, the number of imaging rounds required scales linearly with the number of loci to be imaged. Clever bar-coding approaches, such as SeqFISH + (Takei et al. 2021a, b; Takei et al. 2021a, b), DNA-MERFISH (Multiplexed Error Robust Fluorescence in situ Hybridization) (Su et al. 2020)(Fig. 2C), OligoFISSEQ (fluorescence in situ sequencing) (Nguyen et al. 2020), and in situ genome sequencing (IGS) (Payne et al. 2021), have further revolutionized sequential imaging. Most of these methods rely on combinatorial labeling to massively increase the number of genomic loci that can be probed simultaneously in each cell. For MERFISH, for example, the number of loci that can be imaged scales as ~2N where N is the number of hybridization and imaging rounds (Chen et al. 2015). Termed as spatial omics, these techniques can image large-scale genomic landscapes. When combined with RNA-FISH to detect transcripts and immunofluorescence to detect proteins or histone marks, they provide insights into the organizational principles and epigenetic states of genes within the nucleus, offering single-cell and single-allele resolution (Su et al. 2020; Takei et al. 2021a, b; Takei et al. 2021a, b).
Sequence specific live cell labeling of DNA
DNA-FISH and oligopaints, while invaluable for genomic studies, have a significant drawback: they cannot be used in living cells, thus failing to reveal genome dynamics. Live cell labeling of specific genomic regions is notably challenging (Pradhan et al. 2023). It requires a method that avoids denaturation, and the signal at the target genomic region must be strong enough to be distinguishable from the background. An early solution to these challenges was the use of artificial gene arrays, such as the lac operator (LacO)/lac repressor (LacI) system from Escherichia coli (Tsukamoto et al. 2000). By inserting arrays of LacO into the genome and utilizing a fluorescent protein fusion of LacI, these arrays enable visualization in living cells. However, the LacO array’s random insertion offers no control over the insertion site and can also disrupt chromatin organization.
An alternative approach is the ANCHOR system, which involves inserting a parS sequence into a specific genomic locus (Germier et al. 2018). The ParB protein, fused to a fluorescent protein, then accumulates on the parS sequence due to protein oligomerization. Transcription activator-like effector (TALE) proteins, which can be engineered to bind specific DNA sequences, have also been popular tools for live cell labeling (Miyanari et al. 2013), but it was the advent of clustered regularly interspaced short palindromic repeats (CRISPR)–dCas9 (enzymatically inactive Cas9) that truly revolutionized this field.
CRISPR–CRISPR associated protein 9 (Cas9) has not only revolutionized our ability to precisely edit the genome but the enzymatically inactive Cas9 (dCas9) has quickly been adopted for labeling and visualizing the genome in living cells (Pradhan et al. 2023). Both components of the CRISPR system—the dCas9 fused to a fluorescent protein and the guide RNA —have been used for sequence-specific DNA labeling (Chen et al. 2013; Shao et al. 2016). CRISPR-dCas9 is particularly efficient in labeling the repetitive regions of the genome such as telomeres as only a single guide RNA against the repeating sequence can be used to accumulate and enhance the signal at the genomic locus (Chen et al. 2013). Labeling nonrepetitive sequences poses a greater challenge, initially requiring the delivery of numerous distinct guide RNAs to tile along the locus of interest to sufficiently enhance signal at the target sequence over the background (Chen et al. 2013). However, subsequent advances have started addressing this challenge and improving signal accumulation at genomic sites. Innovations include the use of small multivalent epitope tags, such as SunTag (Neguembor et al. 2018; Tanenbaum et al. 2014), modification of guide RNAs with RNA hairpins (such as MS2 or PP7) (Ma et al. 2015; Qin et al. 2017), and the employment of biomolecular fluorescence complementation with split fluorescent protein systems (Chaudhary et al. 2020; Hong et al. 2018). Additionally, CRISPRainbow, an approach that recruits spectrally distinct fluorescent proteins in a combinatorial fashion by modifying the guide RNA with multiple orthogonal RNA hairpins (MS2, PP7, and boxB) enhanced the ability to simultaneously visualize up to six genomic loci (Ma et al. 2016). Many of the nonrepetitive genome labeling approaches remain challenging to adopt, often requiring complicated strategies that may not always be robust. More developments in easy to implement methods are needed to facilitate the widespread adoption by the scientific community.
Overall, CRISPR-based chromatin labeling stands out as a versatile method. It enables the labeling of multiple genomic regions in different colors and is suitable for various imaging techniques, including live-cell and super-resolution microscopy. This technique has greatly enhanced our ability to visualize and understand the complex organization of the genome in a dynamic cellular environment.
Super-resolution light microscopy of DNA
The development of fluorescence-based DNA probes and advancements in light microscopy have been instrumental in enhancing our understanding of genome dynamics, organization, and function. Traditional light microscopy, however, faces limitations in resolving the ultrastructure of genes, as most genes occupy spaces inside the nucleus that fall below the spatial resolution limit of conventional light microscopes. While chromatin tracing approaches overcome this limitation by localizing each chromatin segment with nanoscale precision, they tend to provide only a simplified ball-and-sticks representation of chromatin and lack information on the presence of specific factors or epigenetic marks within a gene’s spatial vicinity.
It is, therefore, not surprising that the advent of super-resolution light microscopy has significantly bolstered our understanding of the relationship between chromatin structure and function. The principles of super-resolution microscopy methods, including stimulated emission depletion microscopy (STED), and single molecule-based methods, such as stochastic optical reconstruction microscopy (STORM), and DNA point accumulation in nanoscale topography (DNA-PAINT) have been extensively reviewed elsewhere (Bond et al. 2022; Lelek et al. 2021). Many of these methods have been applied to visualize chromatin both globally and at specific gene loci within the nucleus. These methods often require specialized fluorophores with properties such as high brightness, photostability, and, in some cases, the ability to switch between bright and dark states. While some existing DNA dyes have shown compatibility with super-resolution modalities (e.g., PicoGreen for STORM imaging) (Flors 2013; Flors et al. 2009), their photophysical properties are not ideally suited for such applications, limiting their use to proof-of-concept studies. A significant advancement in this regard is the development of STED-compatible SiR-dye derivatives that can be targeted to various cellular structures including DNA, which has enabled high resolution DNA imaging in both living and fixed cells (Lukinavičius et al. 2015). Another approach involves using nucleotide analogues, such as EdU or EdC, which are incorporated into replicating DNA and, upon fixation, can be tagged with a super-resolution compatible fluorophore through click chemistry (Otterstrom et al. 2019; Xiang et al. 2018) (Fig. 1C). This method also extends to labeling and visualizing RNA distribution in relation to chromatin within cells (Castells-Garcia et al. 2022). Super-resolution microscopy has also been used to visualize nucleosomes by labeling the core histone proteins (e.g. H2B and H3), revealing that nucleosomes assemble into irregular clusters at the nanoscale called nucleosome clutches (~30–100 nm) (Ricci et al. 2015). These structures are cell-type dependent and change in response to chemical as well as mechanical cues (Heo et al. 2023; Otterstrom et al. 2019; Ricci et al. 2015). Moreover, epigenetic marks have been observed to enrich within nanoscale domains and the size of these domains change in diseases such as cancer (Cattoni et al. 2017; Xu et al. 2018, 2020).
Beyond global chromatin visualization, super-resolution microscopy combined with oligopaints in techniques such as OligoSTORM (Fig. 2D) has enabled the visualization of individual genes’ volume and compaction level with nanoscale resolution (Beliveau et al. 2015; Boettiger et al. 2016). Studies using OligoSTORM and other super-resolution approaches have shown that transcriptional inhibition can lead to the compaction of gene structures, especially in highly transcribing genes (Neguembor et al. 2021). Additionally, combining OligoSTORM with chromatin tracing has revealed variability in the borders of topologically associating domains (TADs) at the single-cell level, contrasting with the sharp boundaries observed in sequencing-based approaches, such as Hi-C (Bintu et al. 2018).
Although super-resolution methods such as STED and STORM complement the dynamic view of DNA and chromatin from live cell studies, their ability to visualize multiple targets simultaneously is limited due to the need for specialized fluorophores. While this limitation can be overcome using sequential labeling and visualization using the same fluorophore (Tam et al. 2014; Bintu et al. 2018), this approach is slow and low throughput. An emerging super-resolution approach that overcomes this limitation is expansion microscopy (ExM), in which the sample is physically cross-linked to a swellable hydrogel that allows for isotropic expansion upon absorbing water, leading to a physical magnification of the sample. The principles of ExM have been extensively reviewed elsewhere (Wassie et al. 2019; Wen et al. 2023). ExM, which does not require specialized fluorophores or microscopes, simplifies multicolor imaging and has been increasingly used for chromatin visualization. For example, single-cell evaluation of post-translational epigenetic encoding (SCEPTRE) utilizes ExM to visualize genomic loci via DNA-FISH alongside other targets such as histone marks or RNA PolII (Woodworth et al. 2021). This method has revealed correlations between the abundance of active histone marks and RNA PolII at the single-locus level. Chromatin ExM (or chromExM) employed a 15-fold expansion protocol, revealing how transcription factors such as Nanog and RNA PolII interact with nucleosomes in varying configurations in zebrafish embryos during zygotic genome activation (Pownall et al. 2023).
While ExM holds great potential for multimodal imaging of DNA, RNA, and proteins within the nucleus, caution must be taken to ensure that chromatin is preserved at these nanoscale length scales during the expansion process.
Electron microscopy of DNA
Electron microscopy (EM) inherently offers much higher spatial resolution than light microscopy. Some of the earliest insights into chromatin organization were gleaned from electron microscopy images of cell nuclei. These images demonstrated that chromatin segregates into two distinct compartments: an electron-dense heterochromatin compartment, typically enriched at the nuclear lamina, and an electron-poor euchromatin compartment. However, the lack of nucleic acid or protein-specific stains compatible with EM initially resulted in low-contrast images that could not reveal the detailed nucleosome-level folding of chromatin.
A significant advancement in EM of chromatin was the development of an electron microscope-compatible DNA dye (Ou et al. 2017) (Fig. 3A). This dye facilitated the first high-contrast and high-resolution electron microscopy images of chromatin at the nucleosomal level. These ChromEMT (chromatin electron tomography) images corroborated earlier super-resolution findings, showing chromatin as a disordered fiber comprising irregular domains of tightly or loosely packed nucleosomes (Ou et al. 2017). Subsequently, ChromSTEM, which combines DNA-specific staining with scanning electron microscopy, has enabled the analysis of chromatin conformation within chromatin packing domains (Li et al. 2022). It also allows for the quantification of statistical descriptors of chromatin structure relevant to transcriptional activity (Li et al. 2022). Further, serial block-face scanning electron microscopy (SBF-SEM) has recently been combined with in situ hybridization in a method called 3D-EMISH (Trzaskoma et al. 2020) (Fig. 3B). This approach uses biotinylated DNA probes with silver staining to enhance contrast at a specific genomic region for ultrastructural analysis using SBF-SEM (Trzaskoma et al. 2020). This approach has revealed distinct and heterogeneous chromatin-folding structures for megabase scale regions of the genome (Trzaskoma et al. 2020). Some of the drawbacks of these EM-based approaches include the lack of multiple-colors, such as in fluorescence microscopy, and the fact that extensive sample processing and DNA-labeling can potentially compromise sample integrity.
Cryogenic electron microscopy (cryo-EM) methods have also experienced a revolution, bolstered by new detectors and analytical algorithms. More recently, cryo-EM and cryogenic electron tomography (cryoET) has been employed to visualize chromatin in situ at very high resolution across different cell types (Hou et al. 2023; Li et al. 2023; Cai et al. 2018; Jentink et al. 2023; Tan et al. 2023; Beel et al. 2021) (Fig. 3C). Although these cryoET studies are still nascent, they have begun to reveal the heterogeneity in chromatin fiber folding and nucleosome assemblies. They hold significant potential for in situ visualization of native chromatin fibers under various conditions, including both healthy and diseased states.
Conclusions and outlook
The development of probes for visualizing DNA, including histological stains, fluorescent dyes, and FISH probes, coupled with innovative imaging techniques ranging from confocal to super-resolution microscopy, has challenged traditional textbook models of DNA and chromatin organization within the nucleus. We are currently in an exciting era where multiplexed and multimodal approaches are beginning to offer the first detailed views of thousands of genes in relation to their epigenetic and transcriptional states. These high-throughput methods are poised to unravel the longstanding question of how genome structure relates to gene function during critical processes such as development, reprogramming, and disease onset. However, many of these advanced techniques remain confined to specialist labs, limiting broader accessibility for most biologists and, as a result, potentially slowing scientific progress. The commercialization of “smart” automated and multimodal microscopes that facilitate high-throughput studies of genome organization could significantly accelerate advancements in this field. As we continue to acquire more data, the need for higher resolution, increased sensitivity, and the capability to probe the genome structure–function relationship in living cells will become increasingly crucial, driving the development of new technologies.
In parallel with the advancement of these high-throughput imaging technologies, there is a growing need for sophisticated analytical algorithms capable of extracting meaningful information from the vast amount of imaging data generated. In this context, machine learning, deep neural networks, and artificial intelligence are emerging as powerful tools with immense potential. The application of AI in microscope automation for “smart” data acquisition workflows, as well as in image analysis, will likely yield deeper insights into the interplay between genome organization and gene activity. This integrated approach, combining advanced imaging with AI-driven analysis, is poised to revolutionize our understanding of the fundamental principles governing genome structure, function, and regulation.
Data availability
No datasets were generated or analysed during the current study.
References
Bauman JG, Wiegant J, Borst P, van Duijn P (1980) A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA. Exp Cell Res 128:485–490
Beel AJ, Azubel M, Mattei PJ, Kornberg RD (2021) Structure of mitotic chromosomes. Mol Cell 81(4369–76):e3
Beliveau BJ, Joyce EF, Apostolopoulos N, Yilmaz F, Fonseka CY, McCole RB, Chang Y, Li JB, Senaratne TN, Williams BR, Rouillard JM, Wu CT (2012) Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes. Proc Natl Acad Sci USA 109:21301–21306
Beliveau BJ, Boettiger AN, Avendaño MS, Jungmann R, McCole RB, Joyce EF, Kim-Kiselak C, Bantignies F, Fonseka CY, Erceg J, Hannan MA, Hoang HG, Colognori D, Lee JT, Shih WM, Yin P, Zhuang X, Chao-ting Wu (2015) Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes. Nat Commun 6:7147
Bintu B, Mateo LJ, Su JH, Sinnott-Armstrong NA, Parker M, Kinrot S, Yamaya K, Boettiger AN, Zhuang X (2018) Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells. Science. https://doi.org/10.1126/science.aau1783
Boettiger AN, Bintu B, Moffitt JR, Wang S, Beliveau BJ, Fudenberg G, Imakaev M, Mirny LA, Wu CT, Zhuang X (2016) Super-resolution imaging reveals distinct chromatin folding for different epigenetic states. Nature 529:418–422
Bond C, Santiago-Ruiz AN, Tang Q, Lakadamyali M (2022) Technological advances in super-resolution microscopy to study cellular processes. Mol Cell 82:315–332
Brown JM, De Ornellas S, Parisi E, Schermelleh L, Buckle VJ (2022) RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure. Nat Protoc 17:1306–1331
Cai S, Bock D, Pilhofer M, Gan L (2018) The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin. Mol Biol Cell 29:2450–2457
Cardozo Gizzi AM, Cattoni DI, Fiche JB, Espinola SM, Gurgo J, Messina O, Houbron C, Ogiyama Y, Papadopoulos GL, Cavalli G, Lagha M, Nollmann M (2019) Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms. Mol Cell 74(212–22):e5
Castells Garcia A, EEd Daoui IGI, Gonzalez Almela E, Vicario C, Ottestrom J, Lakadamyali M, Cosma MP (2022) Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches. Nucleic Acids Res. https://doi.org/10.1093/nar/gkab1215
Cattoni DI, Cardozo Gizzi AM, Georgieva M, Di Stefano M, Valeri A, Chamousset D, Houbron C, Dejardin S, Fiche JB, Gonzalez I, Chang JM, Sexton T, Marti-Renom MA, Bantignies F, Cavalli G, Nollmann M (2017) Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions. Nat Commun 8:1753
Chambeyron S, Bickmore WA (2004) Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription. Genes Dev 18:1119–1130
Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H (2020) Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore. Genome Res 30:1306–1316
Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B (2013) Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 155:1479–1491
Chen KH, Boettiger AN, Moffitt JR, Wang S, Zhuang X (2015) RNA imaging spatially resolved, highly multiplexed RNA profiling in single cells. Science. https://doi.org/10.1126/science.aaa6090
Chen LF, Long HK, Park M, Swigut T, Boettiger AN, Wysocka J (2023) Structural elements promote architectural stripe formation and facilitate ultra-long-range gene regulation at a human disease locus. Mol Cell 83(1446–61):e6
Chieco P, Derenzini M (1999) The Feulgen reaction 75 years on. Histochem Cell Biol 111:345–358
Cremer T, Cremer M (2010) Chromosome territories. Cold Spring Harb Perspect Biol 2:a003889
de Wit E, Nora EP (2023) New insights into genome folding by loop extrusion from inducible degron technologies. Nat Rev Genet 24:73–85
Espinola SM, Götz M, Bellec M, Messina O, Fiche J-B, Houbron C, Dejean M, Reim I, Cardozo AM, Gizzi ML, Nollmann M (2021) Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early drosophila development. Nat Genet 53:477–486
Finn EH, Misteli T (2021) A high-throughput DNA FISH protocol to visualize genome regions in human cells. STAR Protoc 2:100741
Finn EH, Pegoraro G, Brandao HB, Valton AL, Oomen ME, Dekker J, Mirny L, Misteli T (2019) Extensive heterogeneity and intrinsic variation in spatial genome organization. Cell 176(1502–15):e10
Finn E, Misteli T, Pegoraro G (2022) High-throughput DNA FISH (hiFISH). Methods Mol Biol 2532:245–274
Flors C (2013) Super-resolution fluorescence imaging of directly labelled DNA: from microscopy standards to living cells. J Microsc 251:1–4
Flors C, Ravarani CN, Dryden DT (2009) Super-resolution imaging of DNA labelled with intercalating dyes. ChemPhysChem 10:2201–2204
Franklin RE, Gosling RG (1953) Molecular configuration in sodium thymonucleate. Nature 171:740–741
Gall JG, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A 63:378–383
Germier T, Audibert S, Kocanova S, Lane D, Bystricky K (2018) Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system. Methods 142:16–23
Gomes CJ, Harman MW, Centuori SM, Wolgemuth CW, Martinez JD (2018) Measuring DNA content in live cells by fluorescence microscopy. Cell Div 13:6
Hafner A, Park M, Berger SE, Murphy SE, Nora EP, Boettiger AN (2023) Loop stacking organizes genome folding from TADs to chromosomes. Mol Cell 83(1377–92):e6
Heo S-J, Thakur S, Chen X, Loebel C, Xia B, McBeath R, Burdick JA, Shenoy VB, Mauck RL, Lakadamyali M (2023) Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues. Nature Biomed Eng 7:177–191
Hong Yu, Guangqing Lu, Duan J, Liu W, Zhang Yu (2018) Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging. Genome Biol 19:39
Hou Z, Nightingale F, Zhu Y, MacGregor-Chatwin C, Zhang P (2023) Structure of native chromatin fibres revealed by Cryo-ET in situ. Nat Commun 14:6324
Jentink N, Purnell C, Kable B, Swulius MT, Grigoryev SA (2023) Cryoelectron tomography reveals the multiplex anatomy of condensed native chromatin and its unfolding by histone citrullination. Mol Cell 83(3236–52):e7
Lakadamyali M, Cosma MP (2020) Visualizing the genome in high resolution challenges our textbook understanding. Nat Methods 17:371–379
Lelek M, Gyparaki MT, Beliu G, Schueder F, Griffié J, Manley S, Jungmann R, Sauer M, Lakadamyali M, Zimmer C (2021) Single-molecule localization microscopy. Nature Rev Met Prim 1:39
Li Y, Zhang H, Li X, Wu W, Zhu P (2023) Cryo-ET study from in vitro to in vivo revealed a general folding mode of chromatin with two-start helical architecture. Cell Rep 42:113134
Li Y, Agrawal V, Virk RKA, Roth E, Li WS, Eshein A, Frederick J, Huang K, Almassalha L, Bleher R, Carignano MA, Szleifer I, Dravid VP, Backman V (2022) Analysis of three-dimensional chromatin packing domains by chromatin scanning transmission electron microscopy (ChromSTEM). Sci Rep 12:12198
Lieberman-Aiden E, van Berkum NL, Williams L, Imakaev M, Ragoczy T, Telling A, Amit I, Lajoie BR, Sabo PJ, Dorschner MO, Sandstrom R, Bernstein B, Bender MA, Groudine M, Gnirke A, Stamatoyannopoulos J, Mirny LA, Lander ES, Dekker J (2009) Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326:289–293
Luger K, Mäder AW, Richmond RK, Sargent DF, Richmond TJ (1997) Crystal structure of the nucleosome core particle at 2.8 Å resolution. Nature 389:251–260
Lukinavičius G, Blaukopf C, Pershagen E, Schena A, Reymond L, Derivery E, Gonzalez-Gaitan M, D’Este E, Hell SW, Gerlich DW, Johnsson K (2015) SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy. Nat Commun 6:8497
Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T (2015) Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A 112:3002–3007
Ma H, Li-Chun Tu, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T (2016) Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol 34:528–530
Martin RM, Leonhardt H, Cardoso MC (2005) DNA labeling in living cells. Cytometry A 67:45–52
Mateo LJ, Murphy SE, Hafner A, Cinquini IS, Walker CA, Boettiger AN (2019) Visualizing DNA folding and RNA in embryos at single-cell resolution. Nature 568:49–54
Mello MLS, Vidal BC (2017) The Feulgen reaction: A brief review and new perspectives. Acta Histochem 119:603–609
Millán-Zambrano G, Burton A, Bannister AJ, Schneider R (2022) Histone post-translational modifications — cause and consequence of genome function. Nat Rev Genet 23:563–580
Misteli T (2020) The Self-Organizing Genome: Principles of Genome Architecture and Function. Cell 183:28–45
Miyanari Y, Ziegler-Birling C, Torres-Padilla ME (2013) Live visualization of chromatin dynamics with fluorescent TALEs. Nat Struct Mol Biol 20:1321–1324
Neguembor MV, Martin L, Castells-Garcia A, Gomez-Garcia PA, Vicario C, Carnevali D, AlHaj Abed J, Granados A, Sebastian-Perez R, Sottile F, Solon J, Wu CT, Lakadamyali M, Cosma MP (2021) Transcription-mediated supercoiling regulates genome folding and loop formation. Mol Cell 81(3065–81):e12
Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M (2018) (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res 46:e30
Nguyen HQ, Chattoraj S, Castillo D, Nguyen SC, Nir G, Lioutas A, Hershberg EA, Martins NMC, Reginato PL, Hannan M, Beliveau BJ, Church GM, Daugharthy ER, Marti-Renom MA, C. ting Wu. (2020) 3D mapping and accelerated super-resolution imaging of the human genome using in situ sequencing. Nat Methods 17:822–832
Nir G, Farabella I, Perez Estrada C, Ebeling CG, Beliveau BJ, Sasaki HM, Lee SD, Nguyen SC, McCole RB, Chattoraj S, Erceg J, AlHaj Abed J, Martins NMC, Nguyen HQ, Hannan MA, Russell S, Durand NC, Rao SSP, Kishi JY, Soler-Vila P, Di Pierro M, Onuchic JN, Callahan SP, Schreiner JM, Stuckey JA, Yin P, Aiden EL, Marti-Renom MA, Wu CT (2018) Walking along chromosomes with super-resolution imaging, contact maps, and integrative modeling. PLoS Genet 14:e1007872
Nora EP, Lajoie BR, Schulz EG, Giorgetti L, Okamoto I, Servant N, Piolot T, van Berkum NL, Meisig J, Sedat J, Gribnau J, Barillot E, Bluthgen N, Dekker J, Heard E (2012) Spatial partitioning of the regulatory landscape of the X-inactivation centre. Nature 485:381–385
Nozaki T, Imai R, Tanbo M, Nagashima R, Tamura S, Tani T, Joti Y, Tomita M, Hibino K, Kanemaki MT, Wendt KS, Okada Y, Nagai T, Maeshima K (2017) Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging. Mol Cell 67(282–93):e7
Olins AL, Olins DE (1974) Spheroid chromatin units (v bodies). Science 183:330–332
Otterstrom J, Castells-Garcia A, Vicario C, Gomez-Garcia PA, Cosma MP, Lakadamyali M (2019) Super-resolution microscopy reveals how histone tail acetylation affects DNA compaction within nucleosomes in vivo. Nucleic Acids Res 47:8470–8484
Ou HD, Phan S, Deerinck TJ, Thor A, Ellisman MH, O’Shea CC (2017) ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells. Science. https://doi.org/10.1126/science.aag0025
Park DS, Nguyen SC, Isenhart R, Shah PP, Kim W, Barnett RJ, Chandra A, Luppino JM, Harke J, Wai M, Walsh PJ, Abdill RJ, Yang R, Lan Y, Yoon S, Yunker R, Kanemaki MT, Vahedi G, Phillips-Cremins JE, Jain R, Joyce EF (2023) High-throughput oligopaint screen identifies druggable 3D genome regulators. Nature 620:209–217
Payne AC, Chiang ZD, Reginato PL, Mangiameli SM, Murray EM, Yao CC, Markoulaki S, Earl AS, Labade AS, Jaenisch R, Church GM, Boyden ES, Buenrostro JD, Chen F (2021) In situ genome sequencing resolves DNA sequence and structure in intact biological samples. Science. https://doi.org/10.1126/science.aay3446
Pownall ME, Miao L, Vejnar CE, M’Saad O, Sherrard A, Frederick MA, Benitez MDJ, Boswell CW, Zaret KS, Bewersdorf J, Giraldez AJ (2023) Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin. Science 381:92–100
Pradhan S, Apaydin S, Bucevicius J, Gerasimaite R, Kostiuk G, Lukinavicius G (2023) Sequence-specific DNA labelling for fluorescence microscopy. Biosens Bioelectron 230:115256
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, Darzacq X, Yildiz A, Adli M (2017) Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. Nat Commun 8:14725
Ricci MA, Manzo C, Garcia-Parajo MF, Lakadamyali M, Cosma MP (2015) Chromatin fibers are formed by heterogeneous groups of nucleosomes in vivo. Cell 160:1145–1158
Sen O, Saurin AT, Higgins JMG (2018) The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression. Sci Rep 8:7898
Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y (2016) Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system. Nucleic Acids Res 44:e86
Strom AR, Brangwynne CP (2019) The liquid nucleome - phase transitions in the nucleus at a glance. J Cell Sci. https://doi.org/10.1242/jcs.235093
Su JH, Zheng P, Kinrot SS, Bintu B, Zhuang X (2020) Genome-scale imaging of the 3D organization and transcriptional activity of chromatin. Cell 182(1641–59):e26
Suzuki T, Fujikura K, Higashiyama T, Takata K (1997) DNA staining for fluorescence and laser confocal microscopy. J Histochem Cytochem 45:49–53
Szabo Q, Donjon A, Jerkovic I, Papadopoulos GL, Cheutin T, Bonev B, Nora EP, Bruneau BG, Bantignies F, Cavalli G (2020) Regulation of single-cell genome organization into TADs and chromatin nanodomains. Nat Genet 52:1151–1157
Takei Y, Yun J, Zheng S, Ollikainen N, Pierson N, White J, Shah S, Thomassie J, Suo S, Eng CL, Guttman M, Yuan GC, Cai L (2021a) Integrated spatial genomics reveals global architecture of single nuclei. Nature 590:344–350
Takei Y, Zheng S, Yun J, Shah S, Pierson N, White J, Schindler S, Tischbirek CH, Yuan GC, Cai L (2021b) Single-cell nuclear architecture across cell types in the mouse brain. Science 374:586–594
Tam J, Cordier GA, Borbely JS, Sandoval Alvarez A, Lakadamyali M (2014) Cross-talk-free multi-color STORM imaging using a single fluorophore. PLoS ONE 9:e101772
Tan ZY, Cai S, Noble AJ, Chen JK, Shi J, Gan L (2023) Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ. Elife. https://doi.org/10.7554/eLife.87672
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell 159:635–646
Trzaskoma P, Ruszczycki B, Lee B, Pels KK, Krawczyk K, Bokota G, Szczepankiewicz AA, Aaron J, Walczak A, Śliwińska MA, Magalska A, Kadlof M, Wolny A, Parteka Z, Arabasz S, Kiss-Arabasz M, Plewczyński D, Ruan Y, Wilczyński GM (2020) Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization. Nat Commun 11:2120
Tsukamoto T, Hashiguchi N, Janicki SM, Tumbar T, Belmont AS, Spector DL (2000) Visualization of gene activity in living cells. Nat Cell Biol 2:871–878
van Steensel B, Belmont AS (2017) Lamina-associated domains: links with chromosome architecture heterochromatin, and gene repression. Cell 169:780–791
Wang S, Su JH, Beliveau BJ, Bintu B, Moffitt JR, Wu CT, Zhuang X (2016) Spatial organization of chromatin domains and compartments in single chromosomes. Science 353:598–602
Wassie AT, Zhao Y, Boyden ES (2019) Expansion microscopy: principles and uses in biological research. Nat Methods 16:33–41
Watson JD, Crick FHC (1953) Molecular structure of nucleic acids: a structure for deoxyribose nucleic acid. Nature 171:737–738
Wen G, Leen V, Rohand T, Sauer M, Hofkens J (2023) Current progress in expansion microscopy: chemical strategies and applications. Chem Rev 123:3299–3323
Woodworth MA, Ng KKH, Halpern AR, Pease NA, Nguyen PHB, Kueh HY, Vaughan JC (2021) Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy. Nucleic Acids Res 49:e82
Xiang W, Roberti MJ, Heriche JK, Huet S, Alexander S, Ellenberg J (2018) Correlative live and super-resolution imaging reveals the dynamic structure of replication domains. J Cell Biol 217:1973–1984
Xu J, Ma H, Jin J, Uttam S, Fu R, Huang Y, Liu Y (2018) Super-resolution imaging of higher-order chromatin structures at different epigenomic states in single mammalian cells. Cell Rep 24:873–882
Xu J, Ma H, Ma H, Jiang W, Mela CA, Duan M, Zhao S, Gao C, Hahm ER, Lardo SM, Troy K, Sun M, Pai R, Stolz DB, Zhang L, Singh S, Brand RE, Hartman DJ, Hu J, Hainer SJ, Liu Y (2020) Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis. Nat Commun 11:1899
Acknowledgements
M.L. acknowledges funding from the NIH Common Fund, 4D Nucleome Consortium under the fund numbers U01DA052715, NIH/NIAMS R01 AR079224, and NIH/NIAMS P50 AR080581 and NSF Center for Engineering Mechanobiology under the fund number CMMI-1548571.
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Lakadamyali, M. From feulgen to modern methods: marking a century of DNA imaging advances. Histochem Cell Biol (2024). https://doi.org/10.1007/s00418-024-02291-z
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DOI: https://doi.org/10.1007/s00418-024-02291-z