Abstract
Septoclasts are mononuclear spindle-shaped phagocytes with their long processes in uncalcified cartilage matrices and locate adjacent to the capillary endothelium at the chondro-osseous junction of the growth plate. We have previously revealed a selective expression of epidermal-type fatty acid-binding protein (E-FABP/FABP5) in septoclasts. Although, pericytes are known to distribute along capillaries and directly surround their endothelial cells in a situation similar to septoclasts, no clear evidence is available on the relationship between septoclasts and pericytes. We investigated the chronological localization and morphological change of septoclasts during development of the tibia of mice to clarify the development of septoclasts and the immune-localization of pericyte markers in septoclasts to clarify the origin of septoclasts. E-FABP-immunoreactive septoclasts emerged at the perichondrium in the middle of the cartilaginous templates of the tibia in prenatal development. Septoclasts migrated to the surface of the cartilage adjacent to invading blood vessels. Processes of septoclasts became longer and their apexes attached to Von Kossa-negative uncalcified matrices during the formation process of the primary ossification center. Not only platelet-derived growth factor receptor beta, but also neuron-glial antigen 2 was localized in septoclasts of mice from E15 (embryonic day 15) to P6w (postnatal 6 week). Our results suggest that septoclasts are originated from pericytes and involved in the blood vessel invasion during formation of the primary ossification center.
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Acknowledgements
The authors thank to Dr. Hisatake Kondo (Professor emeritus, Tohoku University) and Dr. Shoichi Iseki (Professor emeritus, Kanazawa University) for their critical advice in manuscript preparation.
Funding
This work was supported by JSPS KAKENHI Grant Numbers JP16K11457 to Y. B. and JP17K11626 to O. A. and Meikai University Miyata Research Grant (2016 A to Y. B.).
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All animal procedures were in accordance with the Guidelines for care and use of Laboratory Animals of Meikai University School of Dentistry, and these experiments were approved by Meikai University Animal Ethics Committee (A1708).
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Bando, Y., Sakashita, H., Taira, F. et al. Origin and development of septoclasts in endochondral ossification of mice. Histochem Cell Biol 149, 645–654 (2018). https://doi.org/10.1007/s00418-018-1653-1
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DOI: https://doi.org/10.1007/s00418-018-1653-1