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Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis

  • Inflammatory Disorders
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Abstract

Background

We evaluated a novel silver amplification immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.

Methods

Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains.

Results

The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed (P < 0.01). A significant correlation was found between AdV DNA copy numbers on a logarithmic scale obtained with the two tests (P < 0.01). The sensitivity of the test kit was 32–64-fold higher than that of the control kit without silver amplification for both AdV types.

Conclusions

These results suggest that this novel amplified AdV detection kit using tears including conjunctival exudate is useful, because it decreases patients’ discomfort from specimen collection and its sensitivity is significantly higher than that of the conventional IC kit.

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Acknowledgements

The clinical strains of adenovirus type 3 and type 54 used for comparative dilution assay were kindly provided by the Kobe Institute of Health.

Funding

This work was supported by a Grant-in-Aid for Encouragement of Scientists (18K09466) from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Migita Eye Clinic, 2-15-13 Harisuri-Chuo, Chikushino, 818-0083, Japan

    Hironori Migita

  2. Department of Ophthalmology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan

    Tomohiro Ueno, Tomoko Tsukahara-Kawamura, Yusuke Saeki & Eiichi Uchio

  3. Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Nozomu Hanaoka & Tsuguto Fujimoto

Authors
  1. Hironori Migita
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  2. Tomohiro Ueno
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  3. Tomoko Tsukahara-Kawamura
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  4. Yusuke Saeki
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  5. Nozomu Hanaoka
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  6. Tsuguto Fujimoto
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  7. Eiichi Uchio
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Corresponding author

Correspondence to Eiichi Uchio.

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Conflict of interest

Authors Migita, Tsukahara-Kawamura, Saeki, and Uchio were provided immunochromatography tests (Quick Chaser Auto Adeno Eye (Nitten Pharmaceutical Co., Ltd., Nagoya, Japan), FUJI DRI-CHEM IMMUNO AG cartridge Adeno OPH (FUJIFILM Corp., Tokyo, Japan)) and immuno reader (Quick Chaser® Immuno Reader (Mizuho Medy Co. Ltd., Tosu, Japan), FUJI DRI-CHEM IMMUNO AG1 (FUJIFILM Corp.)) from each company. Author Ueno declares that he has no conflict of interest. Author Hanaoka declares that he has no conflict of interest. Author Fujimoto declares that he has no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Migita, H., Ueno, T., Tsukahara-Kawamura, T. et al. Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis. Graefes Arch Clin Exp Ophthalmol 257, 815–820 (2019). https://doi.org/10.1007/s00417-019-04281-7

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  • DOI: https://doi.org/10.1007/s00417-019-04281-7

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