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Wide-field contact specular microscopy analysis of corneal endothelium post trabeculectomy

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Abstract

Purpose

This study investigates the possible role of the filtration bleb in the continuous decrease in corneal endothelial cell (CEC) density observed following trabeculectomy.

Methods

This study involved 51 eyes of 37 glaucoma patients who underwent trabeculectomy. The CEC density was determined by contact specular microscopy in three areas: (1) the cornea center, (2) near the trabeculectomy filtration bleb, and (3) the opposite side of the bleb. The eyes were grouped according to post-surgical follow-up years: 0–1 (Group 1), 1–2 (Group 2), 2–3 (Group 3), 3–4, (Group 4), and 4+ years (Group 5).

Results

The mean CEC densities at the opposite side of the bleb, in the cornea center, and near the bleb were 2210 ± 487, 1930 ± 528, and 1519 ± 507 cells/mm2, respectively, in all eyes. The CEC density was significantly lower near the bleb than at the other two sites. The coefficient of variation was significantly higher near the bleb than at the other two sites. The CEC densities at the cornea center and at the opposite side of the bleb showed no significant differences. However, the CEC densities near the bleb showed time-dependent decreases to 1790, 1601, 1407, 1339, and 1224 cells/mm2 for Groups 1, 2, 3, 4, and 5, respectively.

Conclusions

CEC density following trabeculectomy decreased near the bleb, but not at the cornea center, suggesting that the involvement of the filtration bleb in CEC density loss should be further examined to elucidate the pathology of CEC loss following trabeculectomy.

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Funding

No funding was received for this research.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan

    Naoki Okumura, Daiki Matsumoto, Yugo Okazaki & Noriko Koizumi

  2. Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Chie Sotozono, Shigeru Kinoshita & Kazuhiko Mori

  3. Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Shigeru Kinoshita

Authors
  1. Naoki Okumura
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  2. Daiki Matsumoto
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  3. Yugo Okazaki
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  4. Noriko Koizumi
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  5. Chie Sotozono
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  6. Shigeru Kinoshita
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  7. Kazuhiko Mori
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Correspondence to Kazuhiko Mori.

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Conflict of interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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Informed consent was obtained from all individual participants included in the study.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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The authors have no proprietary interest in this study.

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Okumura, N., Matsumoto, D., Okazaki, Y. et al. Wide-field contact specular microscopy analysis of corneal endothelium post trabeculectomy. Graefes Arch Clin Exp Ophthalmol 256, 751–757 (2018). https://doi.org/10.1007/s00417-017-3889-1

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  • DOI: https://doi.org/10.1007/s00417-017-3889-1

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