Skip to main content

Advertisement

SpringerLink
Log in

Evaluation of optic nerve head blood flow in normal rats and a rodent model of non-arteritic ischemic optic neuropathy using laser speckle flowgraphy

  • Basic Science
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To evaluate optic nerve head (ONH) blood flow in normal rats and a rodent model of non-arteritic ischemic optic neuropathy (rNAION) in vivo using laser speckle flowgraphy (LSFG).

Methods

Rats were under general anesthesia; to induce NAION, Rose Bengal (RB) was injected into the tail vein. After the administration of RB, the left ONH was photoactivated using an argon green laser. We measured ONH blood flow in the normal rats and the rNAION group (at 1, 3, 7, 14, and 28 days after the induction of NAION) using an LSFG-Micro. We used the mean blur rate (MBR) of the vessel region (MV) and MBR of the tissue region (MT) as indicators of blood flow. We compared the MBR of the right and left eyes in both the normal rats and the rNAION group.

Results

In the normal rats, there were no significant differences in MV or MT between the right and left eyes. In the rNAION group, the MV and MT of the affected eyes were significantly lower than those of the unaffected eyes at all time points. There were significant differences between the left/right MV and MT ratios seen before the induction of NAION and those observed at 1, 3, 7, 14, and 28 days after the induction of NAION. However, there were no significant differences in these parameters among any of post-NAION induction time points.

Conclusion

Our results indicated that the ONH blood flow of the rNAION rats fell in the acute and chronic phases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Hayreh SS (2008) Ischemic optic neuropathy. Prog Retn Eye Res. 28:34–62

    Article  Google Scholar 

  2. Chuman H (2012) Rodent model of nonarteritic ischemic optic neuropathy and its electrophysiological evaluation. Jpn J Ophthalmol. 56:518–527

    Article  PubMed  Google Scholar 

  3. Bernstein SL (2011) Nonarteritic ischemic optic neuropathy (NAION) and its experimental models. Prog Retin Eye Res. 30:167–187

    Article  PubMed  PubMed Central  Google Scholar 

  4. Maekubo T (2013) Evaluation of inner retinal thickness around the optic disc using optical coherence tomography of a rodent model of nonarteritic ischemic optic neuropathy. Jpn J Ophthalmol. 57(3):327–332

    Article  PubMed  Google Scholar 

  5. Tamaki Y (1997) Real-time measurement of human optic nerve head and choroid circulation using the laser speckle phenomenon. Jpn J Ophthalmol. 41:49–54

    Article  CAS  PubMed  Google Scholar 

  6. Maekubo T (2013) Laser speckle flowgraphy for differentiating between nonarteritic ischemic optic neuropathy and anterior optic neuritis. Jpn J Ophthalmol. 57:385–390

    Article  PubMed  Google Scholar 

  7. Wada Y (2016) Longitudinal Changes in Optic Nerve Head Blood Flow in Normal Rats Evaluated by Laser Speckle Flowgraphy. IOVS. 57(13):5568–5575

    Google Scholar 

  8. Fujii H (1987) Evaluation of blood flow by laser speckle image sensing. Part1. Applied Optics. 26:5321–5325

    Article  CAS  PubMed  Google Scholar 

  9. Sugiyama T (2010) Use of laser speckle flowgraphy in ocular blood flow research. Acta Ophthaomol. 88:723–729

    Article  Google Scholar 

  10. Sugiyama T (2014) Basic technology and clinical applications of the updated model of laser speckle flowgraphy to ocular diseases. Photonics 1:220–234

    Article  Google Scholar 

  11. Collignon-Robe NJ (2004) Optic nerve head circulation in nonarteritic anterior ischemic optic neuropathy and optic neuritis. Ophthalmology. 111:1663–1672

    Article  PubMed  Google Scholar 

  12. Yaoeda K (2000) Measurement of microcirculation in the optic nerve head by laser speckle flowgraphy in normal volunteers. Am J Ophthalmol. 130:606–610

    Article  CAS  PubMed  Google Scholar 

  13. Chuman H (2013) Effects of L-arginine on anatomical and electrophysiological deterioration of the eye in a rodent model of nonarteritic ischemic optic neuropathy. Jpn J Ophthalmol. 57:402–409

    Article  CAS  PubMed  Google Scholar 

  14. Shiga Y (2000) Waveform analysis of ocular blood flow and the early detection of normal tension glaucoma. IOVS 54:7699–7706

    Google Scholar 

  15. Takahashi H (2013) Comparison of CCD-equipped laser speckle flowgraphy with hydrogen gas clearance method in the measurement of optic nerve head microcirculation in rabbits. Exp Eye Res 108:10–15

    Article  CAS  PubMed  Google Scholar 

  16. Arnold AC (2005) Ischemic optic neuropathy. In: Miller NR, Newman NJ (eds) Walsh &Hoyt’s clinical neuro-ophthalmology. Lippincott Williams & Wilkins, Philadelphia, pp 349–384

    Google Scholar 

  17. Mentek M (2015) Compact Laser Doppler Flowmeter (LDF) Fundus Camera for the Assessment of Retinal Blood Perfusion in Small Animals. Plos One 30:10(7)

    Google Scholar 

  18. Hetu S (2013) Assessment of retinal and choroidal blood flow changes using laser Doppler flowmetry in rats. Curr Eye Res 40:36–45

    Google Scholar 

  19. Zhi Z (2011) Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography. Biomed Opt Express 2:579–591

    Article  PubMed  PubMed Central  Google Scholar 

  20. Lorentz K (2008) Scanning laser ophthalmoscope particle tracking method to assess blood flow velocity during hypoxia and hyperoxia. Adv Exp Med Biol 614:253–261

    Article  PubMed  Google Scholar 

  21. Choi W (2012) Measurement of pulsatile total blood flow in the human and rat retina with ultrahigh speed spectral/Fourier domain OCT. Biomed Opt Express 3:1047–1061

    Article  PubMed  PubMed Central  Google Scholar 

  22. Li Y (2008) Blood-flow magnetic resonance imaging of the retina. Neuroimage 39:1744–1751

    Article  PubMed  Google Scholar 

  23. O’Brien C (1997) Effect of chronic inhibition of nitric oxide synthase on ocular blood flow and glucose metabolism in the rat. Br J Ophthalmol 81:68–71

    Article  PubMed  PubMed Central  Google Scholar 

  24. Pouliot M (2009) Quantitative and regional measurement of retinal blood flow in rats using -isopropyl-p-[14C]-iodoamphetamine([14C]-IMP). Exp Eye Res 89:960–966

    Article  CAS  PubMed  Google Scholar 

  25. Wang L (2007) Microspheres method for ocular blood flow measurement in rats: size and dose optimization. Exp Eye Res 84:108–117

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors have presented this article at the 2016 annual ARVO meeting. This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP 15K20269.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki City, Miyazaki Prefecture, 889-1692, Japan

    Hidaka Takako, Chuman Hideki & Nao-i Nobuhisa

Authors
  1. Hidaka Takako
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chuman Hideki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nao-i Nobuhisa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hidaka Takako.

Ethics declarations

Funding

Japan Society for the Promotion of Science (JSPS) KAKENHI provided financial support in the form of Grant-in-Aid for Young Scientists (B) funding. The sponsor had no role in the design or conduct of this research.

Conflict of Interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

The authors declare that they have no conflicts of interest regarding the publication of this paper.

Animal Experiments

Ethical approval: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Takako, H., Hideki, C. & Nobuhisa, Ni. Evaluation of optic nerve head blood flow in normal rats and a rodent model of non-arteritic ischemic optic neuropathy using laser speckle flowgraphy. Graefes Arch Clin Exp Ophthalmol 255, 1973–1980 (2017). https://doi.org/10.1007/s00417-017-3753-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00417-017-3753-3

Keywords

Search

Navigation