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Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy

  • Oculoplastics and Orbit
  • Published:
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Abstract

Background

Thyroid orbitopathy (TO) is a multi-system inflammatory disease characterized by orbital congestion, ocular surface disorders, restrictive myopathy, and skin lesions. The molecular and cellular processes of pathogenic formation of TO orbital fat tissues are not fully understood. In this study, a comparative proteomic analysis was conducted to investigate the importance of some differential proteins of orbital fat tissues in TO.

Methods

The differential proteins were analyzed by comparing the two-dimensional gel electrophoresis (2-DE) maps of the orbital fat tissues of TO with those of normal orbital fat tissues. The 2-DE results were further verified by Western blot and immunohistochemistry.

Results

Fifteen up-regulated and two down-regulated proteins in TO orbital fat tissues in comparison with the control were exhibited by 2-DE maps. The over-expressed proteins including guanine nucleotide-binding protein, isocitrate dehydrogenase (IDH), annexin A2, heat shock protein 60 (HSP 60), calreticulin (CALR), protein disulfide-isomerase A3 (PDIA3), spectrin, superoxide dismutase (SOD), and transitional endoplasmic reticulum ATPase (TER ATPase) may contribute to increased thyroid-stimulating hormone receptor (TSHR) expression and cell proliferation. The proteomic data of specific proteins are consistent with those determined by Western blot and immunohistochemistry.

Conclusions

Comparison of orbital fat proteins from thyroid orbitopathy with age-matched controls shows significant differences in the proteome, and up-regulations of the specific proteins in orbital fat tissues from TO are associated with biochemical mechanisms or capacities against endoplasmic reticulum stress, mitochondria dysfunction, and cell proliferation as well as apoptosis in TO orbital fat tissues.

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Abbreviations

TO:

Thyroid orbitopathy

2-DE:

Two-dimensional gel electrophoresis

TSHR:

Thyroid-stimulating hormone receptor

GPDH:

Glycerol-3-phosphate dehydrogenase

TER ATPase:

Transitional endoplasmic reticulum ATPase

CALR:

Calreticulin

PDIA3:

Protein disulfide-isomerase A3

HSP60:

Heat shock protein 60

IDH:

Isocitrate dehydrogenase

SOD:

Superoxide dismutase

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Acknowledgments

This study was supported by grant kmhk-96-029 from Kaohsiung Municipal Hsiao-kang Hospital.

Conflict of interest

The authors have no conflict of interest.

Author information

Authors and Affiliations

  1. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Kai-Chun Cheng, Jau-Ling Suen & Cheng-Hsien Chang

  2. Department of Ophthalmology, Kaohsiung Municipal Hsiao-kang Hospital, Kaohsiung, Taiwan

    Kai-Chun Cheng, Kuo-Jen Chen & Cheng-Hsien Chang

  3. Department of Ophthalmology, Kaohsiung Medical University Hospital, No.100, Zihyou 1st Road, Sanmin District, Kaohsiung, 807, Taiwan

    Kai-Chun Cheng, Chun-Tzu Hung, Wen-Chuan Wu & Cheng-Hsien Chang

  4. Department of Beauty Science, Meiho University, 23 Pingkung Road, Neipu Hsiang, Pingtung, 91202, Taiwan

    Han-Hsiang Huang, Cheng-Chi Chen & Yu-Jen Wu

  5. Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Wen-Chuan Wu & Cheng-Hsien Chang

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  1. Kai-Chun Cheng
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Correspondence to Yu-Jen Wu or Cheng-Hsien Chang.

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Yu-Jen Wu and Cheng-Hsien Chang are contributed equally to this work

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Cheng, KC., Huang, HH., Hung, CT. et al. Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy. Graefes Arch Clin Exp Ophthalmol 251, 2777–2787 (2013). https://doi.org/10.1007/s00417-013-2446-9

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  • DOI: https://doi.org/10.1007/s00417-013-2446-9

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