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A study on the effect of the internal exposure to 210Po on the excretion of urinary proteins in rats

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Abstract

This study was designed to assess the feasibility of a noninvasive urine specimen for the detection of proteins as indicators of internal exposure to ionizing radiation. Three groups of rats (five in each group) were intravenously injected with 1601 ± 376, 10,846 ± 591 and 48,467 ± 2812 Bq of 210Po in citrate form. A sham-exposed control group of five rats was intravenously injected with sterile physiological saline. Daily urine samples were collected over 4 days following injection. Purification and pre-concentration of urinary proteins were carried out by ultrafiltration using a 3000 Da molecular weight cutoff membrane filter. The concentration of common urinary proteins, namely albumin, alpha-1-acid glycoprotein, immunoglobulins IgA and IgG, was measured by an enzyme-linked immunosorbent assay. Urinary excretion of albumin decreased dose-dependently (p < 0.05) 96 h post-injection relative to the control group. In contrast, no statistically significant effects were observed for other proteins tested. The dose-dependent decrease in urinary excretion of albumin observed in this study underscores the need for further research, which may lead to the discovery of new biomarkers that would reflect the changes in the primary target organs for deposition of 210Po.

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Authors and Affiliations

  1. National Internal Radiation Assessment Section, Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Ottawa, ON, K1A 1C1, Canada

    Baki Sadi, Chunsheng Li, Raymond Ko & Joseph Daka

  2. Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    Hamdi Yusuf

  3. Canadian Nuclear Laboratories, Atomic Energy of Canada Limited, Chalk River, ON, K0J 1J0, Canada

    Heather Wyatt, Joel Surette & Nick Priest

  4. Nuclear Technology Research Laboratory, Radiation Safety Research Center, Central Research Institute of Electric Power Industry (CRIEPI), 2-11-1 Iwado-kita, Komae, Tokyo, 201-8511, Japan

    Nobuyuki Hamada

Authors
  1. Baki Sadi
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  2. Chunsheng Li
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  3. Raymond Ko
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  4. Joseph Daka
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  5. Hamdi Yusuf
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  6. Heather Wyatt
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  7. Joel Surette
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  8. Nick Priest
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  9. Nobuyuki Hamada
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Correspondence to Baki Sadi.

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Sadi, B., Li, C., Ko, R. et al. A study on the effect of the internal exposure to 210Po on the excretion of urinary proteins in rats. Radiat Environ Biophys 55, 161–169 (2016). https://doi.org/10.1007/s00411-016-0639-4

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  • DOI: https://doi.org/10.1007/s00411-016-0639-4

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