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Effect of FK506 on apoptosis of facial motor neurons in rats and its possible mechanism

  • Otology
  • Published:
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Abstract

To investigate the effect of FK506 on apoptosis of facial motor neurons in rats and its possible mechanism. A total of 48 Wistar rats were randomly divided into experimental group and control group. Facial nerve injury model was established by transection of facial nerve at stylomastoid foramen. Rats in experimental group and control group were provided with FK506 and normal saline by intraperitoneal injection, respectively. The morphology of facial neurons was observed under light microscope at different time points after injury. Apoptotic facial motor neurons were detected by TdT-mediated dUTP-biotin nick and labeling (TUNEL) staining, and expression of bcl-2 and bax was evaluated by immunohistochemistry. After facial nerve transection, the apoptotic cells in experimental group significantly decreased compared to control group (P < 0.05), with higher expression of bcl-2 and lower expression of bax in experimental group. FK506 could inhibit apoptosis of facial motor neurons after facial nerve transection, possibly via up-regulation of bcl-2 expression and down-regulation of bax expression.

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Authors and Affiliations

  1. Neuroscience Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, People’s Republic of China

    Ying Zhang & Jing Fan

  2. Department of Geriatrics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, People’s Republic of China

    Ruoyu Zhang

  3. Department of Neurology, Zhejiang Hospital, Hangzhou, People’s Republic of China

    Song Qiao

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  1. Ying Zhang
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  2. Ruoyu Zhang
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  3. Song Qiao
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  4. Jing Fan
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Correspondence to Ying Zhang.

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Zhang, Y., Zhang, R., Qiao, S. et al. Effect of FK506 on apoptosis of facial motor neurons in rats and its possible mechanism. Eur Arch Otorhinolaryngol 273, 601–606 (2016). https://doi.org/10.1007/s00405-015-3591-z

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  • DOI: https://doi.org/10.1007/s00405-015-3591-z

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