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Abnormal expressions of ADAMTS-1, ADAMTS-9 and progesterone receptors are associated with lower oocyte maturation in women with polycystic ovary syndrome

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

ADAMTS-1 and 9 play a crucial role in the ovulation and their altered levels may play a role in the pathogenesis of polycystic ovary syndrome (PCOS). The aim of this study was to assess ADAMTS-1 and 9 expression and their correlation with the oocyte quality and maturity in the cumulus cells (CCs) of PCOS patients and normovulatory women during an IVF procedure.

Methods

Expression of ADAMTS-1 and 9 and progesterone receptors (PRs) in the CCs containing MII and germinal vesicle (GV) oocytes of 37 PCOS patients and 37 women with normal ovulatory function who underwent IVF treatment was evaluated using qRT-PCR. Moreover, correlation between ADAMTS-1 and 9 expression and oocyte quality were also investigated.

Results

mRNA expression levels of ADAMTS-1 and ADAMTS-9 were significantly reduced in the women with PCOS compared to the normovulatory women. ADAMTS-1 and ADAMTS-9 mRNA expression levels in the CCs showed a considerable correlation. Lower expression levels of ADAMTS-1 and ADAMTS-9 in PCOS patients were strongly correlated with diminished oocyte maturation. There was a remarkable association between ADAMTS-1 and ADAMTS-9 mRNA expression levels and oocyte quality. PRs (PRA and PRB) were dramatically decreased in PCOS patients when compared with the control group.

Conclusions

The results of the present study indicated that ADAMTS-1 and ADAMTS-9 as well as PRs are downregulated in the human CCs in PCOS patients, which could be associated with impaired oocyte maturation and may result in a lower oocyte recovery and oocyte maturity rates, as well as lower fertilization rate.

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Acknowledgments

This work was supported by the Research and Technology Deputy of Hamadan University of Medical Sciences (No: 1394.499). We thank the Research Center for Endometrium and Endometriosis and also the Research Center for Molecular Medicine, Hamadan University of Medical Sciences for technical assistance.

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Authors and Affiliations

  1. Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Sepide GohariTaban

  2. Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Iraj Amiri, Sara Soleimani Asl, Mahnaz Yavangi, Nooshin Mohammadpour & Tayebe Artimani

  3. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Massoud Saidijam & Nooshin Shabab

  4. Department of Community Medicine, School of Medicine, Hamadan, Iran

    Elham Khanlarzadeh

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  1. Sepide GohariTaban
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  2. Iraj Amiri
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Contributions

SG project development, IA data collection, SSA data management, MS project development and data management, MY data collection, EK data analysis, NM data collection, NS data collection, TA project development, data management and manuscript writing.

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Correspondence to Tayebe Artimani.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of Hamadan University of Medical Sciences Ethics Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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GohariTaban, S., Amiri, I., Soleimani Asl, S. et al. Abnormal expressions of ADAMTS-1, ADAMTS-9 and progesterone receptors are associated with lower oocyte maturation in women with polycystic ovary syndrome. Arch Gynecol Obstet 299, 277–286 (2019). https://doi.org/10.1007/s00404-018-4967-2

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  • DOI: https://doi.org/10.1007/s00404-018-4967-2

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