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Functionalized acetoacetylated poly(vinyl alcohol) monoliths for enzyme immobilization: a phase separation method

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Abstract

Acetoactylated poly(vinyl alcohol) (AAPVA) monoliths were prepared from polymer solutions via non-solvent-induced phase separation technique for the first time. The morphology of the AAPVA monolith was observed by scanning electron microscopy (SEM) which shown the continuous porous structure existing inside the monolith. A relatively large surface area (102 m2/g) was demonstrated by the Brunauer-Emmett-Teller (BET) analysis. The AAPVA monolith was functionalized by reacting with polyethylenimine (PEI) and glutaraldehyde (GA) successively, through which aldehyde groups were grafted onto the AAPVA monolith. Lipase and horseradish peroxidase (HRP) were successfully immobilized onto the final functionalized AAPVA monolith. On this basis, the present monolith has promising prospect for applications of enzyme immobilization.

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Acknowledgements

This study was funded by the General Financial Grant from the China Postdoctoral Science Foundation (No. 2016M601474), the Fundamental Research Funds for the Central Universities, and “111 Project” Biomedical Textile Materials Science and Technology, China (No. B07024), the Initial Research Funds for Young Teachers of Donghua University, the Natural Science Foundation of Jiangsu Province for Youth (No. BK20160496), and the Scientific Research Foundation of Jiangsu University (No. 16JDG030).

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Authors and Affiliations

  1. College of Textiles, Donghua University, Shanghai, 201620, China

    Xiaoxia Sun & Xinhou Wang

  2. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan

    Xiaoxia Sun, Yuanrong Xin & Hiroshi Uyama

  3. Key Laboratory of Eco-Textile, Ministry of Education, Donghua University, Shanghai, 201620, China

    Xiaoxia Sun & Xinhou Wang

  4. Key Laboratory of Textile Science and Technology, Donghua University, Shanghai, 201620, China

    Xiaoxia Sun

  5. School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China

    Yuanrong Xin

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  1. Xiaoxia Sun
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  2. Yuanrong Xin
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  3. Xinhou Wang
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  4. Hiroshi Uyama
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Correspondence to Hiroshi Uyama.

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Sun, X., Xin, Y., Wang, X. et al. Functionalized acetoacetylated poly(vinyl alcohol) monoliths for enzyme immobilization: a phase separation method. Colloid Polym Sci 295, 1827–1833 (2017). https://doi.org/10.1007/s00396-017-4160-3

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  • DOI: https://doi.org/10.1007/s00396-017-4160-3

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