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The interaction between poly(ε-caprolactone) copolymers containing sulfobetaines and proteins

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Abstract

Copolymers of poly(ε-caprolactone) containing zwitterionic sulfobetaine may be used as biomedical materials. The interaction of these materials with proteins is rarely reported. In this study, amphiphilic copolymers PCL20-PDEAS6, PCL20-PEG200-PDEAS6, and PCL20-PEG400-PDEAS6, which contained poly{N-[2-(methacryloyloxy)ethyl]-N,N-diethyl-N-(3-sulfopropyl)-ammonium} (PDEAS), were designed and synthesized in order to explore interaction of these materials and their micelles with proteins. The structure of the copolymers was confirmed by 1H-NMR, Fourier transform infrared spectroscopy (FTIR), and element analysis (EA). The copolymers could form either films or micelles. The adsorption of bovine serum albumin (BSA) and fibrinogen (Fg) on the copolymer films and their micelles was studied. The results showed that BSA adsorbed on the films presented different tendencies from their micelle formulation. The protein adsorption may be related to the hydration in the shell of the micelles and the electrostatic interaction between the charges of zwitterion and the protein. The reason for the different amounts of protein adsorption between film and micelle formulation is the difference of molecular chain arrangement and charge distribution of PCLDEAS copolymer.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (nos. 51473099 and 51273125). We would also like to appreciate our laboratory members for the generous help and gratefully acknowledge the Analytical and Testing Center at Sichuan University for the tests.

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Authors and Affiliations

  1. College of Polymer Science and Engineering, Sichuan University, Chengdu, Sichuan, 610065, People’s Republic of China

    Aijing Lu, Chenglong Li, Zhengzhong Wu & Xianglin Luo

  2. State Key Laboratory of Polymer Material and Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Xianglin Luo

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Correspondence to Xianglin Luo.

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Lu, A., Li, C., Wu, Z. et al. The interaction between poly(ε-caprolactone) copolymers containing sulfobetaines and proteins. Colloid Polym Sci 294, 1887–1899 (2016). https://doi.org/10.1007/s00396-016-3942-3

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