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Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes

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Abstract

Cationic vesicles fabricated from various novel materials have been recently developed for DNA delivery applications, and the physical stability of the vesicle/DNA complexes played an important role in the transfection efficiency. In this study, positively charged catanionic vesicles composed of hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), ditetradecyldimethylammonium bromide (DTDAB), and cholesterol were prepared in buffer solution by a forced formation approach, and the association behavior of the catanionic vesicles with two types of DNA molecules with the consideration of the vesicle/DNA complex stability was explored by dynamic light scattering, fluorescence polarization, and DNA electrophoresis techniques. In a buffer solution of pH 7.4, HTMA-DS/DTDAB/cholesterol catanionic vesicles with high physical stability and positive charge character were successfully fabricated. A toxicity examination then implied that the catanionic vesicles have the potential of being applied in transfection applications. It is noted that the catanionic vesicle dispersions became unstable as prepared from a concentrated one by dilution due to the less charged vesicle surfaces and the more rigid vesicular bilayers, which could be explained by the enhanced dissolution of HTMA+ from the vesicles. The physical stability and charge character of the catanionic vesicle/DNA complexes were found strongly dependent on the mixing ratio of the vesicle to DNA. The formation of the catanionic vesicle/DNA complexes was also confirmed by an electrophoresis analysis. Moreover, not only the electrostatic repulsion between the vesicle/DNA complexes but also the molecular packing of the vesicular bilayers played roles in governing the physical stability of the vesicle/DNA complexes.

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Acknowledgments

This work was supported by the Ministry of Science and Technology of Taiwan through Grants NSC 97-2221-E-006-094 and NSC-98-2221-E-006-098-MY3. Shu-Chuan Chen is acknowledged for the MTT test.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan

    Chen-Hsuan Lee, Yu-Min Yang & Chien-Hsiang Chang

  2. Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan

    Kuen-Lin Leu & Chia-Hua Liang

  3. Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 811, Taiwan

    Hung-Yin Lin

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  1. Chen-Hsuan Lee
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Correspondence to Chien-Hsiang Chang.

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Lee, CH., Yang, YM., Leu, KL. et al. Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes. Colloid Polym Sci 293, 2239–2247 (2015). https://doi.org/10.1007/s00396-015-3608-6

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  • DOI: https://doi.org/10.1007/s00396-015-3608-6

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