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The anti-hyperuricemic and gut microbiota regulatory effects of a novel purine assimilatory strain, Lactiplantibacillus plantarum X7022

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A Correction to this article was published on 17 April 2024

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Abstract

Purpose

Probiotics have been reported to effectively alleviate hyperuricemia and regulate the gut microbiota. The aim of this work was to study the in vivo anti-hyperuricemic properties and the mechanism of a novel strain, Lactiplantibacillus plantarum X7022.

Methods

Purine content and mRNA expression of purine assimilation related enzymes were determined by HPLC and qPCR, respectively. Hyperuricemic mice were induced by potassium oxonate and hypoxanthine. Uric acid (UA), blood urea nitrogen, creatinine and renal inflammation were examined by kits. The expression of renal UA transporters was subjected to western blotting. Kidney tissues were sectioned for histological analysis. The fecal short-chain fatty acids (SCFAs) were determined by HPLC, and gut microbiota was investigated using the 16S rDNA metagenomic sequencing.

Results

L. plantarum X7022 possesses a complete purine assimilation pathway and can exhaust xanthine, guanine, and adenine by 82.1%, 33.1%, and 12.6%, respectively. The strain exhibited gastrointestinal viability as 44% at the dose of 109 CFU/mL in mice. After four-week administration of the strain, a significant decrease of 35.5% in the serum UA level in hyperuricemic mice was achieved. The diminished contents of fecal propionate and butyrate were dramatically boosted. The treatment also alleviated renal inflammation and restored renal damage. The above physiological changes may due to the inhibited xanthine oxidase (XO) activity, as well as the expressional regulation of UA transporters (GLUT9, URAT1 and OAT1) to the normal level. Notably, gut microbiota dysbiosis in hyperuricemic mice was improved with the inflammation and hyperuricemia related flora depressed, and SCFAs production related flora promoted.

Conclusion

The strain is a promising probiotic strain for ameliorating hyperuricemia.

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Data availability

The datasets generated for this study are available on request to the corresponding author.

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Acknowledgements

This work was supported by National Key Research and Development Program of China (No. 2021YFC2100300; No. 2020YFA0907800), China; Natural Science Foundation of Shanghai (No. 21ZR1416200), China.

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Author notes

  1. Yuan Zou and Kum-Song Ro contributed equally to the work.

Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, Department of Food Science and Engineering, School of Biotechnology, East China University of Science and Technology, P. O. Box 283130 # Meilong Rd, Shanghai, 200237, People’s Republic of China

    Yuan Zou, Kum-Song Ro, Chentian Jiang, Deyi Yin, Li Zhao, Lei Du & Jingli Xie

  2. Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, 210042, Jiangsu, People’s Republic of China

    Daihui Zhang

  3. Department of Biotechnology, Faculty of Life Science, Kim Hyong Jik University of Education, Pyongyang, Democratic People’s Republic of Korea

    Kum-Song Ro

  4. Shanghai Collaborative Innovation Center for Biomanufacturing (SCICB), Shanghai, 200237, People’s Republic of China

    Jingli Xie

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Contributions

ZY and ZL analyzed main data and wrote the main manuscript text; JCT and YDY prepared figures and tables; RKS and ZDH helped with the methodology; DL and XJL reviewed and edited manuscript text. All authors reviewed the manuscript.

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Correspondence to Lei Du.

Additional information

The original online version of this article was revised: Correct article note “Yuan Zou and Kum-Song Ro contributed equally to the work” has been updated.

Supplementary Information

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394_2023_3291_MOESM1_ESM.docx

Supplementary file1 (DOCX 22 KB) The primers used for real-time quantitative polymerase chain reaction (RT-qPCR) are shown in Table S1.

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Zou, Y., Ro, KS., Jiang, C. et al. The anti-hyperuricemic and gut microbiota regulatory effects of a novel purine assimilatory strain, Lactiplantibacillus plantarum X7022. Eur J Nutr 63, 697–711 (2024). https://doi.org/10.1007/s00394-023-03291-w

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