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Administration of alpha-lipoic acid could maintain bone mass and bone strength in senile female rats with alcohol consumption

Die Gabe von Alpha-Liponsäure könnte die Knochenmasse und Knochenstärke bei alten weiblichen Ratten mit Alkoholkonsum erhalten

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Abstract

Previous studies have demonstrated the damaging effect of alcohol (ALH) consumption on bone tissue and bone metabolism. Alpha-lipoic acid (ALA) promotes osteoblast proliferation and inhibits osteoclast proliferation, and positively affects bone regeneration; however, reports about effects of ALA on bone loss for aged female rats with ALH consumption are limited. This study was designed to investigate the impact of treatment with ALA on bone loss for aged female rats with ALH consumption. In this study 30 female Sprague–Dawley rats (22 months old), weighing approximately 520 g, were incorporated. The animals were randomly divided into three groups: group CON, group ALH and group ALH + ALA and received saline, ALH, ALH plus ALA treatment until death at 16 weeks, respectively. The results of maintaining bone mass and bone strength in senile female rats with ALH consumption were evaluated by histology, microcomputerized tomography, gene expression analysis and biomechanical tests. Results from this study indicated that ALH + ALA had stronger effects on the prevention and treatment of osteoporosis in senile female rats with ALH consumption. The ALH + ALA produced stronger effects on the bone volume ratio (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp), BMD and strength of distal femurs, and regulation of osteogenesis and bone resorption-related gene expression. These results seem to indicate that ALA intervention prevents bone loss in senile female rats with ALH consumption.

Zusammenfassung

Frühere Studien haben den schädigenden Effekt von Alkohol (ALH) auf Knochengewebe und -stoffwechsel gezeigt. Alpha-Liponsäure (ALA) fördert die Proliferation von Osteoblasten, hemmt die Proliferation von Osteoklasten und hat einen positiven Effekt auf die Knochenregeneration. Berichte über die Auswirkungen von ALA auf den Knochenverlust in alten weiblichen Ratten mit ALH-Konsum sind limitiert. Diese Studie wurde durchgeführt, um den Effekt der ALA-Behandlung auf den Knochenverlust bei alten weiblichen Ratten mit ALH-Konsum zu untersuchen. In diese Studie wurden 30 weibliche Spraque-Dawley-Ratten (22 Monate alt) mit einem Gewicht von ungefähr 520 g eingeschlossen. Die Tiere wurden in drei Gruppen randomisiert: Gruppe CON, Gruppe ALH und Gruppe ALH + ALA, die jeweils Kochsalzlösung, ALH bzw. eine Behandlung mit ALH + ALA bis zum Tod nach 16 Wochen erhielten. Die Ergebnisse für den Erhalt von Knochenmasse und Knochenstärke bei alten weiblichen Ratten mit ALH-Konsum wurden histologisch sowie mittels Mikrocomputertomographie, Genexpressionsanalyse und biomechanischen Tests evaluiert. Die Resultate dieser Studie zeigten, dass ALH + ALA einen stärkeren Effekt auf die Prävention und Behandlung von Osteoporose bei alten weiblichen Ratten mit ALH-Konsum hatte. ALH + ALA hatte einen größeren Effekt auf BV/TV (bone volume ratio), Tb.Th (trabecular thickness), Tb.N (trabecular number) und Tb.Sp (trabecular separation), BMD sowie die Stärke des distalen Femurs, die Osteogeneseregulation und die knochenresorptionsbedinge Genexpression. Diese Ergebnisse scheinen darauf hinzuweisen, dass die Behandlung mit ALA bei alten weiblichen Ratten mit ALH-Konsum einen Knochenverlust verhindert.

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Authors and Affiliations

  1. Department of orthopaedics Surgery, The Second Hospital of Anhui Medical University, Hefei, China

    Junfeng Zhan, Nan Zhu & Wang Fang

  2. Department of Orthopaedics, Hefei Third People’s Hospital, Hefei, China

    Ya Jiang

  3. Department of orthopaedics Surgery, Nanfang Hospital Southern Medical University, Guangzhou, China

    Gang Wang

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  1. Junfeng Zhan
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  2. Ya Jiang
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  3. Nan Zhu
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  5. Gang Wang
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Correspondence to Gang Wang.

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J. Zhan, Y. Jiang, L. Zhu, W. Fang and G. Wang declare that they have no competing interests.

All procedures performed in studies involving animal testing were in accordance with the ethical standards of the institutional and/or national research committee and with the 1975 Helsinki declaration and its later amendments or comparable ethical standards.

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Junfeng Zhan and Ya Jiang contributed equally to this work and share the first author.

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Zhan, J., Jiang, Y., Zhu, N. et al. Administration of alpha-lipoic acid could maintain bone mass and bone strength in senile female rats with alcohol consumption. Z Gerontol Geriat 53, 679–686 (2020). https://doi.org/10.1007/s00391-019-01630-3

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