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Tracheal cartilage growth promotion by intra-tracheal administration of basic FGF

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Abstract

Purpose

This study aimed to investigate whether intra-tracheal administration of basic fibroblast growth factor (b-FGF) promotes the growth of tracheal cartilage.

Methods

Trachea of 4-week old mice were intubated and 2.5 μg b-FGF administered (Group 4) for periods from 1 to 5 days. Cervical tracheal outer diameter and tracheal ring length were compared in Group 1 (no intervention), Group 2 (tracheal intubation), Group 3 (intra-tracheal administration of distilled water) and Group 4, at 8 weeks of age. Outer diameter and tracheal ring length in Group 4 were also compared with that in Group 1 at 12 and 16 weeks of age.

Results

At 8 weeks of age, tracheal ring length with b-FGF administration for more than 4 days in Group 4 was significantly increased over that following 1-day administration. At 8 weeks of age, mean outer diameter and the mean tracheal ring length in Group 4 were significantly greater than in the other groups. Mean outer diameter and mean tracheal ring length were significantly greater in Group 4 than in Group 1 at 12 and 16 weeks of age.

Conclusion

This study has shown that intra-tracheal administration of b-FGF enlarges the tracheal lumen.

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Acknowledgments

This study was supported by grants from Kawano Masanori Memorial Foundation for Promotion of Pediatrics 2009 and the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 22591977). We appreciate the technical support provided by Nobuyuki Kikuchi, who is a technician in the Department of Tissue Engineering in the Graduate School of Medicine, University of Tokyo The authors give special thanks to Kaken Pharmaceutical Co. Ltd. for providing human recombinant fibroblast growth factor-2 (b-FGF) for cell culture.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Makoto Komura, Hiroko Komura, Tetsuya Ishimaru, Kenichiro Konishi & Hiroaki Komuro

  2. Department of Pediatric Surgery, Saitama Medical University, Morohongou 38, Moroyama-chou, Iruma-gun, Saitama, 350-0495, Japan

    Makoto Komura

  3. Department of Tissue Engineering, Tokyo University Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Makoto Komura, Kazuto Hoshi & Tsuyoshi Takato

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  1. Makoto Komura
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  2. Hiroko Komura
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Contributions

MK, HK, TI and KK performed the experiments. MK, HK, HK and TI designed the research study. KH and TT contributed essential tools. MK, HK, KH, TT and TI analysed the data, and MK and HK wrote the paper.

Corresponding author

Correspondence to Makoto Komura.

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Conflict of interest

Makoto Komura received a contributed human recombinant fibroblast growth factor-2 (b-FGF) for cell culture from Kaken Pharmaceutical Co. Ltd.

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Komura, M., Komura, H., Ishimaru, T. et al. Tracheal cartilage growth promotion by intra-tracheal administration of basic FGF. Pediatr Surg Int 36, 33–41 (2020). https://doi.org/10.1007/s00383-019-04576-0

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  • DOI: https://doi.org/10.1007/s00383-019-04576-0

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