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Accelerated intestinal epithelial cell turnover after bowel resection in a rat is correlated with inhibited hedgehog signaling cascade

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Abstract

Purpose

The hedgehog (Hh) signaling pathway is one of the key regulators of gastrointestinal tract development. Recent studies point to the role of hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of intestinal stem cells. The purpose of this study was to evaluate the role of Hh signaling during intestinal adaptation in a rat model of short bowel syndrome (SBS).

Methods

Male rats were divided into two groups: Sham rats underwent bowel transection and SBS rats underwent a 75 % bowel resection. Parameters of intestinal adaptation, enterocyte proliferation, and apoptosis were determined 2 weeks after operation. Illumina’s Digital Gene Expression analysis was used to determine the Hh signaling gene expression profiling. Hh-related genes and protein expression were determined using Real-Time PCR, Western blotting, and immunohistochemistry.

Results

Massive small bowel resection resulted in a significant increase in enterocyte proliferation and concomitant increase in cell apoptosis. From the total number of 20,000 probes, 13 genes related to Hh signaling were investigated. In jejunum, eight genes were down-regulated, three genes up-regulated, and two genes remained unchanged. In ileum, five genes were down-regulated and six genes were unchanged in SBS vs sham animals. SBS rats also demonstrated a significant three- to fourfold decrease in SMO, GIL, and PTCH mRNA, and protein levels (determined by Real-Time PCR and Western blot) compared to control animals.

Conclusion

Two weeks following massive bowel resection in rats, the accelerated cell turnover was accompanied by an inhibited Hh signaling pathway. Hh signaling may serve as an important mediator of reciprocal interactions between the epithelium and the underlying mesenchymal stroma during intestinal adaptation following massive bowel resection in a rat.

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Authors and Affiliations

  1. Laboratory of Intestinal Adaptation and Recovery, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

    I. Sukhotnik, T. Dorfman, Salim Halabi, Y. Pollak & H. Kreizman Shefer

  2. Department of Pediatric Surgery B, Bnai Zion Medical Center, 47 Golomb St., P.O.B. 4940, Haifa, 31048, Israel

    I. Sukhotnik & T. Dorfman

  3. Department of Emergency Medicine, Carmel Medical Center, Haifa, Israel

    Salim Halabi

  4. Department of Pediatric Gastroenterology and Nutrition, Bnai Zion Medical Center, Haifa, Israel

    D. Berkowitz

  5. Section of Pediatric Surgery, C.S. Mott Children’s Hospital, University of Michigan Medical School, Ann Arbor, MI, USA

    A. G. Coran

Authors
  1. I. Sukhotnik
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  2. T. Dorfman
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  3. Salim Halabi
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  4. Y. Pollak
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  5. H. Kreizman Shefer
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  6. A. G. Coran
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  7. D. Berkowitz
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Corresponding author

Correspondence to I. Sukhotnik.

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Sukhotnik, I., Dorfman, T., Halabi, S. et al. Accelerated intestinal epithelial cell turnover after bowel resection in a rat is correlated with inhibited hedgehog signaling cascade. Pediatr Surg Int 32, 1133–1140 (2016). https://doi.org/10.1007/s00383-016-3969-z

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  • DOI: https://doi.org/10.1007/s00383-016-3969-z

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