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Placental membrane grafts for urethral replacement in a rabbit model: a pilot study

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Abstract

Purpose

Several graft materials are available for use in the treatment of urethral stricture disease. Placental membrane is being used in a variety of settings as a graft in wound healing and tissue repair. We aim to evaluate the effect of implanting decellularized human placental membrane into rabbit urethras.

Methods

Dorsal onlay graft urethroplasty using prepared human placental membrane was performed in 10 New Zealand White rabbits (Oryctolagus cuniculus). After 3 months, the rabbits underwent cystourethroscopy to evaluate urethral patency. The rabbits were then euthanized and the urethras examined for pathological findings.

Results

All urethroplasties were performed without complication. There were no observed episodes of urinary retention, infection, or renal failure. Urethral patency was achieved in all rabbits 3 months postoperatively. Urothelial replacement of the placental membrane graft was observed in all rabbits without malignant transformation.

Conclusion

Dorsal onlay urethroplasty using decellularized human placental membrane can safely be performed in a rabbit model. This pilot study demonstrated urothelial replacement of human placental membrane in the rabbit urethra without stricture formation. Placental membrane is a promising biomaterial for urethral reconstruction.

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Funding

Institutional. Prepared placental membranes provided by LifeNet Health® (Virginia Beach, VA).

Author information

Authors and Affiliations

  1. Department of Urology, Naval Medical Center San Diego, 34800 Bob Wilson Drive, San Diego, CA, 32134, USA

    Chad R. Pusateri, Alexander D. Doudt, Steven Gauerke & Jack M. Zuckerman

  2. Department of Urology, Eastern Virginia Medical School, 225 Clearfield Avenue, Virginia Beach, VA, 23462, USA

    Kurt McCammon

  3. LifeNet Health®, 1864 Concert Drive, Virginia Beach, VA, 23453, USA

    Xiaofei Qin, Britini Ork & Joseph M. Khoury

  4. Division of Animal Resources, Naval Medical Center San Diego, 34800 Bob Wilson Drive, San Diego, CA, 32134, USA

    Anthony D. May

  5. Department of Pathology, Naval Medical Center San Diego, 34800 Bob Wilson Drive, San Diego, CA, 32134, USA

    Steven Gauerke

Authors
  1. Chad R. Pusateri
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  2. Alexander D. Doudt
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  3. Steven Gauerke
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  4. Kurt McCammon
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  5. Xiaofei Qin
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  6. Britini Ork
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  7. Joseph M. Khoury
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  8. Anthony D. May
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  9. Jack M. Zuckerman
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Contributions

CRP: protocol/project development, data collection or management, data analysis, and manuscript writing/editing. ADD: protocol/project development, data collection or management, data analysis, and manuscript writing/editing. SG: data collection or management, data analysis, and manuscript writing/editing. KM: protocol/project development. XQ: protocol/project development and manuscript writing/editing. BO: protocol/project development. JMK: protocol/project development and manuscript writing/editing. ADM: data collection or management and manuscript writing/editing. JMZ: protocol/project development, data collection or management, data analysis, and manuscript writing/editing.

Corresponding author

Correspondence to Jack M. Zuckerman.

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Conflict of interest

The views expressed in this manuscript are those of the authors and do not reflect the official policy or position of the Department of the Navy, Department of Defense, or the United States Government. Xiaofei Qin, Britini Ork, Joseph M. Khoury are employees of LifeNet Health® (Virginia Beach, VA). The remaining authors declare that they have no relevant financial interests. This abstract was presented as a podium presentation at AUA 2018.

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Pusateri, C.R., Doudt, A.D., Gauerke, S. et al. Placental membrane grafts for urethral replacement in a rabbit model: a pilot study. World J Urol 38, 2133–2138 (2020). https://doi.org/10.1007/s00345-019-02836-1

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  • DOI: https://doi.org/10.1007/s00345-019-02836-1

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