Abstract
Broccoli microgreens represent a novel type of vegetable characterized by high levels of bioactive compounds. Here, we investigated the effects of light-emitting diode (LED) light quality on the development and nutritional uptake of broccoli microgreens. The plants were exposed to five distinct light treatments: white, single red, and red: blue at 5:1, 5:3, and 5:5 ratios. The soluble sugars, nutrients, and secondary metabolites of broccoli sprout extracts were examined using high-performance liquid chromatography and ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry under various light-quality conditions. In addition, the expression levels of genes encoding important enzymes in the glucosinolates (GLs) biosynthesis pathway were examined using quantitative real-time polymerase chain reaction. Overall, the findings demonstrated that mixed red-blue light, especially the 5:1 red: blue ratio, was beneficial for broccoli microgreens in terms of their hypocotyl length, fresh weight, edible rate, soluble proteins, vitamin C, total phenolics, total flavonoids, and GLs. Our findings provide a foundation and reference point for selecting the most appropriate LED light ratio in microgreens production.
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References
Ani NN, Ahmad R, Zain CRCM (2014) Effect of RGB LED pulse lights in photomorphogenesis of Brassica chinensis. In: 2nd international conference on agriculture and biotechnology, Phuket, Thailand
Brazaitytė A, Miliauskienė J, Vaštakaitė-Kairienė V, Sutulienė R, Laužikė K, Duchovskis P, Małek S (2021) Effect of different ratios of blue and red led light on Brassicaceae microgreens under a controlled environment. Plants (Basel) 10:801. https://doi.org/10.3390/plants10040801
Cartea ME, Francisco M, Soengas P, Velasco P (2010) Phenolic compounds in Brassica vegetables. Molecules 16:251–280. https://doi.org/10.3390/molecules16010251
Craver JK, Gerovac JR, Lopez RG, Kopsell DA (2017) Light intensity and light quality from sole-source light-emitting diodes impact phytochemical concentrations within brassica microgreens. J Am Soc Hortic Sci 142:3–12
Chen X, Cai W, Xia J, Yu H, Wang Q, Pang F, Zhao M (2020) Metabolomic and transcriptomic analyses reveal that blue light promotes chlorogenic acid synthesis in strawberry. J Agric Food Chem 68:12485–12492. https://doi.org/10.1021/acs.jafc.0c05020
Choe U, Yu LL, Wang TTY (2018) The science behind microgreens as an exciting new food for the 21st century. J Agric Food Chem 66:11519–11530. https://doi.org/10.1021/acs.jafc.8b03096
Demotes-Mainard S, Péron T, Corot A, Bertheloot J, Le Gourrierec J, Pelleschi-Travier S, Crespel L, Morel P, Huché-Thélier L, Boumaza R, Vian A, Guérin V, Leduc N, Sakr S (2016) Plant responses to red and far-red lights, applications in horticulture. Environ Exp Bot 121:4–21. https://doi.org/10.1016/j.envexpbot.2015.05.010
Gigolashvili T, Berger B, Flügge U-I (2009) Specific and coordinated control of indolic and aliphatic glucosinolate biosynthesis by R2R3-MYB transcription factors in Arabidopsis thaliana. Phytochem Rev 8:3–13. https://doi.org/10.1007/s11101-008-9112-6
Jones MA (2018) Using light to improve commercial value. Hortic Res 5:47
Kadri H, Djilani SE, Djilani A (2013) Phytochemical constituents, antioxidant activity, total phenolic and flavonoid contents of Arisarum vulgare seeds. Acta Sci Pol Technol Aliment 12:169–173
Kopsell DA, Sams CE, Barickman TC, Morrow RC (2014) Sprouting broccoli accumulate higher concentrations of nutritionally important metabolites under narrow-band light-emitting diode lighting. J Am Soc Hortic Sci 139:469–477
Kyriacou MC, El-Nakhel C, Graziani G, Pannico A, Soteriou GA, Giordano M, Ritieni A, Pascale SD, Rouphael Y (2019) Functional quality in novel food sources: genotypic variation in the nutritive and phytochemical composition of thirteen microgreens species. Elsevier, New York
Lee MJ, Son JE, Oh MM (2014) Growth and phenolic compounds of Lactuca sativa L. grown in a closed-type plant production system with UV-A,-B, or-C lamp. J Sci Food Agric 94:197–204. https://doi.org/10.1002/jsfa.6227
Metallo RM, Kopsell DA, Sams CE, Bumgarner NR (2018) Influence of blue/red vs. white LED light treatments on biomass, shoot morphology, and quality parameters of hydroponically grown kale. Sci Hortic 235:189–197. https://doi.org/10.1016/j.scienta.2018.02.061
Murphy CJ, Pill WG (2015) Cultural practices to speed the growth of microgreen arugula (roquette; Eruca vesicaria subsp. sativa). J Pomol Hortic Sci 85:171–176
Pająk P, Socha R, Gałkowska D, Rożnowski J, Fortuna T (2014) Phenolic profile and antioxidant activity in selected seeds and sprouts. Food Chem 143:300–306. https://doi.org/10.1016/j.foodchem.2013.07.064
Pérez-Balibrea S, Moreno DA, García-Viguera C (2008) Influence of light on health-promoting phytochemicals of broccoli sprouts. J Sci Food Agric 88:904–910. https://doi.org/10.1002/jsfa.3169
Qian H, Liu T, Deng M, Miao H, Cai C, Shen W, Wang Q (2016) Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chem 196:1232–1238. https://doi.org/10.1016/j.foodchem.2015.10.055
Riggio GMW, QingKniel KEGibson, Kristen E, (2019) Microgreens—a review of food safety considerations along the farm to fork continuum. Int J Food Microbiol 290:76–85. https://doi.org/10.1016/j.ijfoodmicro.2018.09.027
Saltveit ME (2017) Synthesis and metabolism of phenolic compounds. In: Fruit and vegetable phytochemicals: chemistry and human health, 2nd edn. pp 115–124
Samuolienė G, Brazaitytė A, Jankauskienė J, Viršilė A, Sirtautas R, Novičkovas A, Sakalauskienė S, Sakalauskaitė J, Duchovskis P (2013) LED irradiance level affects growth and nutritional quality of Brassica microgreens. Cent Eur J Biol 8:1241–1249. https://doi.org/10.2478/s11535-013-0246-1
Schuster J, Knill T, Reichelt M, Gershenzon J, Binder S (2006) Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis. Plant Cell 18:2664–2679. https://doi.org/10.1105/tpc.105.039339
Seo JM, Arasu MV, Kim YB, Park SU, Kim SJ (2015) Phenylalanine and LED lights enhance phenolic compound production in Tartary buckwheat sprouts. Food Chem 177:204–213. https://doi.org/10.1016/j.foodchem.2014.12.094
Sønderby IE, Geu-Flores F, Halkier BA (2010) Biosynthesis of glucosinolates–gene discovery and beyond. Trends Plant Sci 15:283–290. https://doi.org/10.1016/j.tplants.2010.02.005
Taulavuori E, Taulavuori K, Holopainen JK, Julkunen-Tiitto R, Acar C, Dincer I (2017) Targeted use of LEDs in improvement of production efficiency through phytochemical enrichment. J Sci Food Agric 97:5059–5064. https://doi.org/10.1002/jsfa.8492
Tian Y, Liimatainen J, Alanne AL, Lindstedt A, Liu P, Sinkkonen J, Kallio H, Yang B (2017) Phenolic compounds extracted by acidic aqueous ethanol from berries and leaves of different berry plants. Food Chem 220:266–281. https://doi.org/10.1016/j.foodchem.2016.09.145
Vaštakaitė V, Viršilė A, Brazaitytė A, Samuolienė G, Jankauskienė J, Sirtautas R, Novičkovas A, Dabašinskas L, Sakalauskienė S, Miliauskienė J (2015) The effect of blue light dosage on growth and antioxidant properties of Brassicaceae microgreens. In: Nordic view to sustainable rural development: 25th NJF congress, June 16–18, 2015. Nordic Association of Agricultural Scientists (NJF): proceedings. Riga: NJF
Weber CF (2017) Broccoli microgreens: a mineral-rich crop that can diversify food systems. Front Nutr 4:7. https://doi.org/10.3389/fnut.2017.00007
Wind J, Smeekens S, Hanson J (2010) Sucrose: metabolite and signaling molecule. Phytochemistry 71:1610–1614. https://doi.org/10.1016/j.phytochem.2010.07.007
Wu B-S, Hitti Y, MacPherson S, Orsat V, Lefsrud MG (2020) Comparison and perspective of conventional and LED lighting for photobiology and industry applications. Environ Exp Bot 171:103953. https://doi.org/10.1016/j.envexpbot.2019.103953
Xiao Z, Lester GE, Luo Y, Wang Q (2012) Assessment of vitamin and carotenoid concentrations of emerging food products: edible microgreens. J Agric Food Chem 60:7644–7651. https://doi.org/10.1021/jf300459b
Xue A, Liang W, Wen S, Gao Y, Huang X, Tong Y, Hao Y, Luo L (2021) Metabolomic analysis based on EESI-MS indicate blue LED light promotes aliphatic-glucosinolates biosynthesis in broccoli sprouts. J Food Compos Anal 97:103777. https://doi.org/10.1016/j.jfca.2020.103777
Yeh N, Ding TJ, Yeh P (2015) Light-emitting diodes׳ light qualities and their corresponding scientific applications. Renew Sustain Energy Rev 51:55–61. https://doi.org/10.1016/j.rser.2015.04.177
Zhang J, Luo W, Zhao Y, Xu Y, Song S, Chong K (2016) Comparative metabolomic analysis reveals a reactive oxygen species-dominated dynamic model underlying chilling environment adaptation and tolerance in rice. New Phytol 211:1295–1310. https://doi.org/10.1111/nph.14011
Zhang X, Bian Z, Yuan X, Chen X, Lu C (2020) A review on the effects of light-emitting diode (LED) light on the nutrients of sprouts and microgreens. Trends Food Sci Technol 99:203–216. https://doi.org/10.1016/j.tifs.2020.02.031
Acknowledgements
This study was supported by the National Natural Science Foundation of China (Grant Number 32360646), and the Jiangxi Natural Science Foundation Project (Grant Numbers 20232BAB205010, 20212AEI91010, 20192BAB204014).
Funding
Funding was provided by National Natural Science Foundation of China (Grant Number 32360646), Natural Science Foundation of Jiangxi Province (Grant Numbers 20232BAB205010, 20192BAB204014), and Jiangxi Natural Science Foundation Project (Grant Number 20212AEI91010).
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LLP: Conceptualization, formal analysis, investigation, methodology, software, validation, writing—original draft, writing—review and editing, visualization. ZGW: Investigation, formal analysis, validation, writing—review and editing. LWJ: Investigation, project administration, resources. WD: Data curation, supervision, funding acquisition, project administration, resources, writing—review and editing. SQF: Data curation, supervision, funding acquisition, project administration, resources. HYB: Conceptualization, methodology, supervision, funding acquisition, project administration, resources, writing—review and editing, visualization.
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Luo, L., Zhang, G., Liang, W. et al. Effects of LED Light Quality on Broccoli Microgreens Plant Growth and Nutrient Accumulation. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11326-7
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DOI: https://doi.org/10.1007/s00344-024-11326-7