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Plant Growth-Promoting and Arsenic Accumulation Reduction Effects of Two Endophytic Bacteria Isolated from Brassica napus

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Abstract

Infertile and heavy-metal-contaminated croplands are widely distributed and threaten global food security. Plant growth-promoting bacteria provide an option for alleviating the agricultural problems. In this study, the endophytic bacteria Rahnella victoriana B38 (Accession No. OK658118) and Bacillus paramycoides N38 (Accession No. OK658562) were isolated from Brassica napus. Their plant growth-promoting effects and mechanisms were investigated by gnotobiotic and pot experiments, and extracellular metabolites analysis. The results revealed that both B38 and N38 significantly increased shoot and root length in B. napus and fresh weight, root length, pod number, and total pod weight of Arabidopsis thaliana (p < 0.05). In addition, they promoted Arabidopsis growth under arsenic (As) stress by significantly increasing fresh weight and root length and decreasing the As content by 55.81% and 30.47%, respectively (p < 0.05). Thus, these bacteria were able to protect Arabidopsis from As stress by reducing As accumulation in plants. B38 and N38 release gamma-aminobutyric acid (GABA) and enzymes including proteases, cellulases, and lipases, which can increase soil nutrient availability. Substances related to plant biomass increasing and As-stress alleviation, including indoleacetic acid (IAA), GABA, putrescine, quinic acid, trans-zeatin, N-acetylglutamic, 3-indolepropionic acid, putrescine, and lathyrine were also detected. Therefore, they could represent ideal microbial biofertilizer candidates for increasing crop yields and alleviating As accumulation in plants.

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Acknowledgements

We are thankful to Dr. Jiang Liu from Institute of Geochemistry, Chinese Academy of Sciences for the assistance with the language. This work was supported by the Natural Science Foundation Project of CQ CSTC (Grant No. cstc2018jcyjAX0629) to Caiyun Yang, National Natural Science Foundation of China (Grant No. 31600095) to Caiyun Yang, and Chongqing Research Institutions Performance Incentive Guide Project (Grant No. cstc2020jxjl20002) to Li Fan.

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  1. Chongqing Key Laboratory of Bio-Resource Development for Bioenergy and Bioremediation, College of Resources and Environment, Southwest University, Chongqing, 400715, China

    Wenying Chang, Xiping Hou, Yaoyao Yan, Ting Liu, Xianzhu Dai, Yasuo Igarashi, Caiyun Yang & Feng Luo

  2. Chongqing Research Academy of Eco-Environmental Sciences, Chongqing, 400715, People’s Republic of China

    Li Fan

  3. State Cultivation Base of Eco-Agriculture for Southwest Mountainous Land, Department of Environmental Sciences and Engineering, College of Resource and Environment, Southwest University, Chongqing, 400716, China

    Caiyun Yang

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  1. Wenying Chang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by WC and CY. Data collection were performed by XH, YY, and TL. Experimental design was performed by XD and CY. Reagent and manuscript revise were provided by LF and YI. The first draft of the manuscript was written by WC and all authors commented on the previous versions of the manuscript. In particular, additional experiments (i.e., detection of IAA produced from B38 and N38 under As stress, and microscopic observation of bacterial colonization on the roots of Arabidopsis under normal and As-stress conditions) were performed by LF during the revised manuscript preparing. And she also partially contributed to the manuscript corrections. Thus, LF was added as a co-author of this manuscript in the revised version. We would like to thank Editage (www.editage.cn) for English language editing. All authors read and approved the final manuscript.

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Correspondence to Caiyun Yang or Feng Luo.

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Chang, W., Hou, X., Yan, Y. et al. Plant Growth-Promoting and Arsenic Accumulation Reduction Effects of Two Endophytic Bacteria Isolated from Brassica napus. J Plant Growth Regul 43, 76–88 (2024). https://doi.org/10.1007/s00344-023-11056-2

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