Abstract
Small ubiquitin-like modifier (SUMO) conjugation to target proteins is an important post-translational modification, which regulates plant tolerance to biotic and abiotic stresses. SIZ1, a well-characterized SUMO E3 ligase, facilitates the conjugation of SUMO to target proteins. Here, a SIZ/PAIS-type protein SlSIZ2 was identified in tomato (Solanum lycopersicum) that is a homolog of AtSIZ1 and SlSIZ1. SlSIZ2 was expressed in tomato vegetative and reproductive tissues, and induced by ABA and NaCl. Nucleus-localized SlSIZ2 partially rescued atsiz1-2 dwarfism and also alleviated the sensitivity of atsiz1-2 to ABA and NaCl, suggesting the functional replacement of SlSIZ2 to AtSIZ1. Moreover, SlSIZ2-overexpressing Arabidopsis has higher cotyledon expansion rate, lateral root density and survival rate under salinity stress. These results suggested the contribution of SlSIZ2 to the tolerance of salinity stress.
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Acknowledgements
We thank Dr. Hongliang Zhu and Jianrong Ye (China Agricultural University, China) for kindly providing Ailsa Craig tomato seeds. This work was supported by the Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (Grant No. cstc2020jcyj-msxmX0180) and the Fundamental Research Funds for the Central Universities (Grant No. XDJK2019D025).
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MZ and YFH designed the research; SL, JQ and QW performed the experiments; QW, MZ and YFH analyzed the data; QW, MZ and YFH wrote the manuscript; all authors read and approved the manuscript.
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Sequence data from this article can be found in the GenBank database or TAIR (The Arabidopsis Information Resource) under the following accession numbers: SlSIZ2 (Solyc06g010000.2), SlSIZ1 (Solyc11g069160.3.1), SlCAC (Solyc08g006960.3.1), AtSIZ1 (AT5G60410), AtACT2 (AT3G18780), AtP5CS1 (AT2G39800), AtTUB2 (AT5G62690), AtNHX1 (AT5G27150), AtHKT1 (AT4G10310), AtSOS1 (AT2G01980), AtSOS2 (AT5G35410), AtSOS3 (AT5G24270).
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Wang, Q., Lei, S., Qian, J. et al. Overexpression of Tomato SIZ2 in Arabidopsis Improves Plant Salinity Tolerance. J Plant Growth Regul 42, 1893–1904 (2023). https://doi.org/10.1007/s00344-022-10667-5
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DOI: https://doi.org/10.1007/s00344-022-10667-5