Abstract
Grafting is an important way to improve crop growth, stress resistance and yield. However, the molecular mechanism of grafting affecting flowering is not clear. In this study, roots of Paeonia broteri (PGPB) and Paeonia ostii (PGPO) were grafted with Paeonia suffruticosa ‘Luoyang Hong’ as the experimental material to measure transcriptome of the petals at a full blooming stage. A total of 41.84 Gb clean data were obtained; 80,390 unigenes and 439 differentially expressed genes (DEGs) were identified. Functional classification and enrichment of DEGs indicated that the differences in gene expression between PGPB and PGPO were mainly reflected in starch and sucrose metabolism, cell wall polysaccharides modification, redox activity, and signal transduction process. Differential expression of the hormone, protein kinase and ROS-related genes reflected the different degrees of body response of tree peony petals to different rootstocks. Besides, qRT-PCR analysis of expression profiles of the selected DEGs was in agreement with the results from RNA-seq analysis. This study investigated the molecular response of tree peony petals to grafting with two different rootstocks and laid the foundation for further study on the molecular mechanism of grafting to improve plant growth and development.
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This work is financially supported by the National Natural Science Foundation of China (31600568) and the Henan Agricultural University Doctoral Fund Project (30500565).
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Yan Li designed the experiment; Yihong Chang performed bioinformatics analysis on transcriptome data and wrote the manuscript; Jiuxing Lu helped in the experiments and data analysis; Rui Wang and Dan He performed the determination of physiological indicators and qRT-PCR. Qiusheng Yang confirmed the research content, controlled the progress of the test and the quality of the manuscript writing. Yonghua Li reviewed and edited the manuscript.
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Li, Y., Chang, Y., Lu, J. et al. Comparative Transcriptome Analysis of Tree Peony Petals on Two Different Rootstocks. J Plant Growth Regul 38, 1287–1299 (2019). https://doi.org/10.1007/s00344-019-09933-w
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DOI: https://doi.org/10.1007/s00344-019-09933-w