Abstract
The genetic control of the floral transition in winter-flowering chrysanthemum is not well investigated. Here, RNA-seq technology was applied to explore the transcriptome of the variety ‘QD026’ at five developmental time points between the fully vegetative stage and flowering. The number of clean reads obtained per library was around 5 × 107. Under short-day growing conditions, floral induction was accompanied by a substantial number of differentially transcribed genes: these included constans-like and embryonic flower in the photoperiod pathway, and flowering locus c, short vegetative phase, and squamosa promoter-binding protein-like genes in the ambient temperature pathway. A quantitative PCR assay was successfully used to validate the differential transcription of a sample of the genes identified by the RNA-seq analysis as being differentially transcribed.
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Acknowledgements
This work was supported by funding from the National Natural Science Foundation of China (31372100), the Jiangsu Province “Programs of Innovation and Entrepreneurship Talents,” and the Jiangsu Higher Education Institutions Priority Academic Program Development.
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Supplementary Figure 1. Quantitative real-time PCR (qRT-PCR) validation of differentially transcribed genes (DTGs) from RNA-Seq in the ‘QD026’ transcriptome. Correlation of fold change analyzed by RNA-Seq platform (x axis) with data obtained using qRT-PCR (y axis)
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Supplementary Table 1. Dates of ‘QD026’ plants to an 8 h photoperiod at constant temperature of 20℃ until the first appearance of visible buds Temperature and photoperiod treatment started on 5.29, visible capitulum of Chrysanthemum morifolium ‘QD026’ was formed on 7.8 ± 2. Data given in the form mean ± SEM
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Supplementary Table 2. Differentially transcribed genes homologous to those known to act in the photoperiod pathway Hx vs H0 calculated from |log2Ratio|(Hx/H0)|
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Supplementary Table 3. Differentially transcribed members of the WRKY family in the ‘QD026’ transcriptome Hx vs H0 calculated from |log2Ratio|(Hx/H_0)|
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Supplementary Table 4. Differentially transcribed members of the CCCH family in the ‘QD026’ transcriptome Hx vs H0 calculated from |log2Ratio|(Hx/H_0)|
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Supplementary Table 5. Differentially transcribed members of the bHLH family in the ‘QD026’ transcriptome Hx vs H0 calculated from |log2Ratio|(Hx/H_0)|
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Supplementary Table 6. Differentially transcribed members of the MYB family in the ‘QD026’ transcriptome Hx vs H0 calculated from |log2Ratio|(Hx/H_0)|
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Cheng, P., Dong, B., Wang, H. et al. A Transcriptomic Analysis Targeting Genes Involved in the Floral Transition of Winter-Flowering Chrysanthemum. J Plant Growth Regul 37, 220–232 (2018). https://doi.org/10.1007/s00344-017-9720-8
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DOI: https://doi.org/10.1007/s00344-017-9720-8