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Characterization of Ripening-Related PuARP4 in Pear (Pyrus ussuriensis)

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Abstract

The ‘Nanguo’ pear (Pyrus ussuriensis) fruit is typically climacteric, and ethylene is the main factor controlling the ripening of climacteric fruit. Whether the actin cytoskeleton is involved in ethylene-mediated fruit ripening remains unclear. In this study, we characterized an actin-related protein, PuARP4. The expression of PuARP4 was evaluated in young leaves, stems, flowers, and roots as well as in fruits. Expression of PuARP4 decreased during fruit development and ripening, and it was inhibited by Ethephon treatment but induced by 1-MCP treatment. To explore the network of PuARP4 function in ‘Nanguo’ pear fruit ripening, we screened a cDNA library from ‘Nanguo’ pear fruits using PuARP4 as bait. PuPME1 (pectin methylesterase 1) was identified as a potential interactor of PuARP4; PuPME1 has been found to degrade the pectin of cell walls. This direct interaction was further confirmed by a yeast two-hybrid system and pull-down analyses. Analysis of the expression of PuPME1 showed that it could be regulated by ethylene. Our results indicated that PuARP4 was involved in ethylene-mediated fruit ripening and might cooperate with PuPME1 to regulate the ripening process. Our results provide a new link between fruit ripening and the cytoskeleton and will provide a new platform for research on ethylene-mediated fruit ripening. The possible mechanisms underlying this process are discussed.

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Authors and Affiliations

  1. College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China

    Hui Yuan, Lichao Zhang, Zhongyu Jiang & Aide Wang

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  1. Hui Yuan
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  2. Lichao Zhang
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  3. Zhongyu Jiang
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  4. Aide Wang
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Correspondence to Aide Wang.

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Yuan, H., Zhang, L., Jiang, Z. et al. Characterization of Ripening-Related PuARP4 in Pear (Pyrus ussuriensis). J Plant Growth Regul 36, 766–772 (2017). https://doi.org/10.1007/s00344-017-9680-z

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  • DOI: https://doi.org/10.1007/s00344-017-9680-z

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