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Structural characterization of sulfated galactofucan from Undaria pinnatifida and its effect on type 2 diabetic mice

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Abstract

A sulfated galactofucan designated as UpG was obtained from the brown algae Undaria pinnatifida by calcium chloride extraction. Chemical analyses showed that UpG is composed of galactose and fucose at a high sulfation level. Low-molecular weight UpGP-0.5 was prepared from UpG through acid hydrolysis for structure characterization. The backbones of UpG are determined to be α-(1,3)-Fuc, α-(1,4)-Gal, α-(1,3)-Gal, and α-(1,6)-Gal by GC-MS, FT-IR, NMR, and LC-MS analyses. Sulfate groups are modified at C2 and/or C4 of fucose and C3 and/or C4 of galactose. UpG could partially lower blood sugar and serum lipid levels in type 2 diabetic mice. Moreover, UpG treatment regulates the abundance of some specific gut microbiota, such as enriching the abundance of Muribaculum and Christensenellaceae, and reducing that of Bilophila, Tannerellaceae, Candidatus Saccharimonas and Anaerotruncus. The findings characterized the detailed structure of a sulfated galactofucan and investigated its potential for the treatment of type 2 diabetes mellitus.

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Data Availability Statement

The data that support the finding of this study are available from the corresponding author.

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Author notes

  1. Songze KE and Bo ZHANG contributed equally to this work and should be regarded as co-first authors.

Authors and Affiliations

  1. College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Moganshan Research Institute at Deqing County Zhejiang University of Technology, Zhejiang University of Technology, Hangzhou, 310014, China

    Songze Ke, Bo Zhang, Yanlei Yu, Sijia Wang, Jianwei Chen, Huawei Zhang, Bin Wei & Hong Wang

  2. Center for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, CA, 90024, USA

    Sijia Wang

  3. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China

    Weihua Jin

  4. Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou, 310014, China

    Yanlei Yu, Jianwei Chen, Huawei Zhang, Bin Wei & Hong Wang

  5. Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou, 310014, China

    Jian Wu

Authors
  1. Songze Ke
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  2. Bo Zhang
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  3. Yanlei Yu
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  9. Bin Wei
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  10. Hong Wang
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Contributions

Songze KE and Bo ZHANG analyzed the data and drafted the manuscript. Yanlei YU and Sijia WANG helped with the biological experiments. Weihua JIN and Huawei ZHANG provided valuable intellectual input. Jianwei CHEN and Jian WU provided polysaccharide sample. Hong WANG and Bin WEI designed the experiment and revised the manuscript.

Corresponding authors

Correspondence to Bin Wei or Hong Wang.

Additional information

Supported by the National Key Research and Development Program (Nos. 2018YFC0311003, 2017YFE0103100), the National Natural Science Foundation of China (Nos. 81903534, 81773628, 81741165), and the High-Level Talent Special Support Plan of Zhejiang Province (No. 2019R52009)

Supporting Information

The 2D spectra 1H-1H COSY, 1H-1H TOCSY and HMBC of UpGP-0.5 (Supplementary Fig. S1). Negative-ion mode ESI- MS spectra of the oligosaccharide fractions U1, U2, U3, U4, U5 and U6 (Supplementary Fig.S2).

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Ke, S., Zhang, B., Yu, Y. et al. Structural characterization of sulfated galactofucan from Undaria pinnatifida and its effect on type 2 diabetic mice. J. Ocean. Limnol. 41, 300–313 (2023). https://doi.org/10.1007/s00343-021-1307-3

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