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Biosynthesis of phycocyanobilin in recombinant Escherichia coli

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Abstract

The recombinant expression of phycocyanobilin (PCB) was carried out in Escherichia coli, and the best fermentation conditions of recombinant E. coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted, isolated, and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy, high-pressure liquid chromatography, and mass spectrometry. In addition, the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include: lactose concentration 4 mmol/L, induction temperature 24.69°C, induction time 4.6 h, and induction duration 13.57 h, under which the PCB expression level reached approximately 13 mg PCB/L, which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals.

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Data Availability Statement

The data supporting the findings of this study are shown in this article. Otherwise, please contact the author for more information.

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Author notes

  1. MA Chengbo and LI Wenjun contributed equally to this work and should be regarded as co-first authors.

Authors and Affiliations

  1. College of Life Sciences, Yantai University, Yantai, 264005, China

    Chengbo Ma & Jian Lin

  2. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Wenjun Li & Song Qin

  3. Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266580, China

    Baosheng Ge

  4. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China

    Wenjun Li & Song Qin

Authors
  1. Chengbo Ma
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  2. Wenjun Li
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  3. Baosheng Ge
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  4. Jian Lin
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  5. Song Qin
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Corresponding authors

Correspondence to Jian Lin or Song Qin.

Additional information

Supported by the National Natural Science Foundation of China (No. 41906109) and the Key Research and Development Program of Yantai (No. Y933021011).

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Ma, C., Li, W., Ge, B. et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli. J. Ocean. Limnol. 38, 529–538 (2020). https://doi.org/10.1007/s00343-019-9060-6

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  • DOI: https://doi.org/10.1007/s00343-019-9060-6

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