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Effects of varying densities on serum reproductive parameters in pen-reared juvenile female rainbow trout Oncorhynchus mykiss farms

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An Erratum to this article was published on 07 March 2017

Abstract

The primary goal of this study was to assess the effect of varying densities on serum reproductive parameters of immature rainbow trout Oncorhynchus mykiss. Experimental trout were maintained in intensive, pen-reared farms for 300 days in fresh water reservoirs. Initial densities were 4.6, 6.6, and 8.6 kg/m3 (40, 60, 80 ind./m3), indicated as SD1, SD2, SD3, and final densities were 31.1, 40.6, 49.3 kg/m3, respectively. A summary of the ovarian stages were observed by histological examination. Serum E2 (estradiol), T (testosterone) were evaluated by radioimmunoassay and FSH (follicle-stimulating-hormone), LH (luteinizing-hormone), vitellogenin, 17α,20β-P (17α,20βdihydroxy4-pregnen-3-one) were measured by enzyme-linked immunosorbent assay. Our findings demonstrated that ovarian development were retarded (from stage III to stage IV) at highest rearing density (SD3) after 180 days of intensive culture (over 40.6 kg/m3). In addition, we observed an inverse relationship between serum reproductive parameters and rearing density. Furthermore, compared to serum reproductive parameters of SD1, E2, T, FSH, vitellogenin, 17α,20β-P, GSI and LH of two higher density groups decreased firstly and significantly at 60 (over 15.9 kg/m 3 ), 180 (over 31.7 kg/m 3 ), 180 (over 40.6 kg/m3), 240 (over 36 kg/m3), 240 (over 36 kg/m3), 240 (over 45 kg/m3) and 300 (over 49.3 kg/m3) days, respectively. Comparing serum reproductive parameters within the same ovarian development stage of rainbow trout from varying densities revealed that higher population density also led to significantly lower overall serum reproductive parameters. Overall, this study presents the reproductive, endocrinological parameters of juvenile female rainbow trout at high rearing densities and indicates the need for rainbow trout (114.44±5.21 g, 19.69±0.31 cm) that are initially stocked at 6.6 or 8.6 kg/m3 should be classified and subdivided into lower density after 180 days of farming (not over 31.7 kg/m3).

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Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao, 266003, China

    Zhishuai Hou  (侯志帅), Haishen Wen  (温海深), Jifang Li  (李吉方), Feng He  (何峰), Qun Liu  (刘群), Jinhuan Wang  (王金环), Biao Guan  (管标) & Qinglong Wang  (王庆龙)

  2. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Zhishuai Hou  (侯志帅), Haishen Wen  (温海深), Jifang Li  (李吉方), Feng He  (何峰), Qun Liu  (刘群), Jinhuan Wang  (王金环), Biao Guan  (管标) & Qinglong Wang  (王庆龙)

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  1. Zhishuai Hou  (侯志帅)
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  2. Haishen Wen  (温海深)
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  3. Jifang Li  (李吉方)
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  4. Feng He  (何峰)
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  6. Jinhuan Wang  (王金环)
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  7. Biao Guan  (管标)
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  8. Qinglong Wang  (王庆龙)
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Correspondence to Haishen Wen  (温海深).

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Supported by the Special Fund for Agro-Scientific Research in the Public Interest (No. 201003055)

An erratum to this article is available at http://dx.doi.org/10.1007/s00343-017-7466-6.

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Hou, Z., Wen, H., Li, J. et al. Effects of varying densities on serum reproductive parameters in pen-reared juvenile female rainbow trout Oncorhynchus mykiss farms. Chin. J. Ocean. Limnol. 35, 98–108 (2017). https://doi.org/10.1007/s00343-016-5172-4

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  • DOI: https://doi.org/10.1007/s00343-016-5172-4

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