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Ultrafast fluorescence spectroscopy for axial resolution of flurorophore distributions

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Abstract

A new method for determining the fluorophore distribution along the propagation axis of an ultrashort optical pulse is presented. The axial resolution is obtained by temporal gating of the backward emitted fluorescence via optical parametric amplification, and we demonstrated a resolution in the order of a few 100 μm. With this approach, sampling of the fluorophore concentration of thin layers without using optics with a large numerical aperture will be possible, such as investigating the human retina via time-resolved fluorescence measurements. Additionally, we verified the gain is orders of magnitude higher for coherent seeding, making optical parametric gating very interesting for discriminating between coherently and incoherently scattered light for other multimodal imaging applications.

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References

  1. D. Schweitzer, A. Kolb, M. Hammer, R. Anders, Der Ophthalmol. 99, 774–779 (2002)

    Article  Google Scholar 

  2. M. Hammer, E. Nagel, D. Schweitzer, S. Richter, F. Schweitzer, E. Königsdörffer, J. Strobel, Der Ophthalmol. 101, 1189–1193 (2004)

    Article  Google Scholar 

  3. D. Schweitzer, B. Beuermann, M. Hammer, F. Schweitzer, S. Richter, L. Leistritz, M. Scibor, E. Thamm, A. Kolb, E. Anders, Klinisches Monatsblatt Augenheilkunde 222, 396–408 (2005)

    Article  Google Scholar 

  4. M. Hammer, E. Königsdörffer, C. Liebermann, C. Framme, G. Schuch, D. Schweitzer, J. Strobel, Graefe’s Arch. Clin.Exp. Ophthalmol. 246, 105–114 (2008)

    Article  Google Scholar 

  5. B.R. Masters, Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell Imaging (SPIE Press Monograph, Bellingham, 2006), p. 230

    Book  Google Scholar 

  6. M. Yanoff, J.S. Duker, Ophthalmology, 2nd edn. (Mosby/Elsevier, Philadelphia, 2004)

    Google Scholar 

  7. G. Saxby, The Science of Imaging, 2nd edn. (CRC Press, Boca Raton, 2011)

    Google Scholar 

  8. J. Pawley, Handbook of Biological Confocal Microscopy, 3rd edn. (Springer Science, New York, 2010)

    Google Scholar 

  9. W. Drexler, Optical Coherence Tomography (Springer, New York, 2008)

    Book  Google Scholar 

  10. M. de Groot, C.L. Evans, J.F. de Boer, Opt. Express 20, 15253–15262 (2012)

    Article  ADS  Google Scholar 

  11. J.R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, New York, 2006)

    Book  Google Scholar 

  12. A.C. Bhasikuttan, T. Okada, J. Phys. Chem. B 108, 12629–12632 (2004)

    Article  Google Scholar 

  13. R. Schanz, S.A. Kovalenko, Kharlanov, N.P. Ernsting, Appl. Phys. Lett. 79, 566–568 (2001)

  14. L. Zhao, J.L. Pérez, Lustres, Farztdinov, N.P. Ernsting, Phys. Chem. Chem. Phys. 7, 1716–1725 (2005)

  15. M.O. Lenz, R. Huber, B. Schmidt, P. Gilch, R. Kalmbach, M. Engelhard, J. Wachtveitl, Biophys. J. 91, 255–262 (2006)

    Article  ADS  Google Scholar 

  16. U. Förster, N. Gildenhoff, C. Grünewald, J.W. Engels, J. Wachtveitl, J. Lumin. 129, 1454–1458 (2009)

    Article  Google Scholar 

  17. X.-X. Zhang, C. Würth, L. Zhao, U. Resch-Genger, N.P. Ernsting, M. Sajadi, Rev. Sci. Inst. 82, 063108–1–063108–8 (2011)

    Google Scholar 

  18. I. Martini, G.V. Hartland, Chem. Phys. Lett. 258, 180–186 (1996)

    Article  ADS  Google Scholar 

  19. S. Akimoto, I. Yamazaki, S. Takaichi, M. Mimoru, Chem. Phys. Lett. 313, 63–68 (1999)

    Article  ADS  Google Scholar 

  20. P. Fita, Y. Stepanenko, C. Radzewicz, Appl. Phys. Lett. 86, 021909–1–021909–2 (2005)

    Article  Google Scholar 

  21. X.-F. Han, X.-H. Chen, Y.-X. Weng, J.-Y. Zhang, J. Opt. Soc. Am. B 24, 1633–1638 (2007)

    Article  ADS  Google Scholar 

  22. H. Chen, Y. Weng, J. Zhang, J. Opt. Soc. Am. B 26, 1627–1634 (2009)

    Article  ADS  Google Scholar 

  23. H.-L. Chen, Y.-X. Weng, X.-Y. Li, Chin. J. Chem. Phys. 24, 253–255 (2011)

    Article  Google Scholar 

  24. H. Chen, W. Dang, J. Xie, J. Zhao, Y. Weng, Photosynth. Res. 111, 81–86 (2012)

    Article  Google Scholar 

  25. P. Fita, C. Radzewicz, J. Opt. Soc. Am. B 25, 1625–1626 (2008)

    Article  ADS  Google Scholar 

  26. D. Schweitzer, M. Klemm, M. Hammer, S. Jentsch, F. Schweitzer, Proc. SPIE 7368, 736804 (2009)

    Article  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge support from the European Regional Development Fund (EFRE) and the state of Thuringia (TMBWK). Furthermore, we thank Dr. Schweitzer from the Department of Ophthalmology for bringing our attention to this interesting topic. We also acknowledge experimental support from Dr. Schweitzer and his coworkers.

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Authors and Affiliations

  1. Institute of Optics and Quantum Electronics, Abbe Center of Photonics, Friedrich-Schiller-University Jena, Max-Wien-Platz 1, 07743, Jena, Germany

    Maximilian G. O. Gräfe, Andreas Hoffmann & Christian Spielmann

  2. Helmholtzinstitut Jena, Fröbelstieg 3, 07743, Jena, Germany

    Christian Spielmann

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  1. Maximilian G. O. Gräfe
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  2. Andreas Hoffmann
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  3. Christian Spielmann
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Correspondence to Maximilian G. O. Gräfe.

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Gräfe, M.G.O., Hoffmann, A. & Spielmann, C. Ultrafast fluorescence spectroscopy for axial resolution of flurorophore distributions. Appl. Phys. B 117, 833–840 (2014). https://doi.org/10.1007/s00340-014-5894-y

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