Abstract
Due to the high importance of Staphylococcus aureus alpha-toxin in poisoning and pathogenicity of this bacterium, we developed a sensitive and selective sensor based on gold nanoparticle/multi-walled carbon nanotubes modified carbon paste electrode and 1-butyl-3-methylimidazolium hexafluorophosphate as a binder for electrochemical detection of alpha-toxin. The prepared nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, dynamic light scattering, energy dispersive X-ray analysis, and UV–visible spectra. The square wave voltammetry as a sensitive technique was selected for the quantification of alpha-toxin. Electrochemical behavior of unmodified and modified electrode, the type and pH value of supporting electrolyte, and scan rate were done for quantification of alpha-toxin. To determine the specificity of the aptasensor, the interference of some serum markers were also examined. The linear range and detection limit were calculated at 3.0–250.0 nM and 1.0 nM, respectively. The relative standard deviations for 5.0 nM and 100.0 nM alpha-toxin were obtained 1.15–0.83%, respectively. None of the interfering factors were involved in measuring the concentration of 120.0 nM alpha-toxin. Therefore, the introduced aptasensor with high sensitivity, selectivity, and repeatability, was used for the determination of alpha-toxin in the serum sample.
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Vakyly, S., Sedighian, H., Jahromi, Z. et al. A sensitive and selective electrochemical sensor based on gold nanoparticle/multi-walled carbon nanotubes for detection of Staphylococcus aureus Alpha-toxin. Appl. Phys. A 128, 680 (2022). https://doi.org/10.1007/s00339-022-05822-4
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DOI: https://doi.org/10.1007/s00339-022-05822-4