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OsPPR11 encoding P-type PPR protein that affects group II intron splicing and chloroplast development

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Abstract

Key message

OsPPR11 belongs to the P-type PPR protein family and can interact with OsCAF2 to regulate Group II intron splicing and affect chloroplast development in rice.

Abstract

Pentatricopeptide repeat (PPR) proteins participate in chloroplasts or mitochondria group II introns splicing in plants. The PPR protein family contains 491 members in rice, but most of their functions are unknown. In this study, we identified a nuclear gene encoding the P-type PPR protein OsPPR11 in chloroplasts. The qRT-PCR analysis demonstrated that OsPPR11 was expressed in all plant tissues, but leaves had the highest expression. The osppr11 mutants had yellowing leaves and a lethal phenotype that inhibited chloroplast development and photosynthesis-related gene expression and reduced photosynthesis-related protein accumulation in seedlings. Moreover, photosynthetic complex accumulation decreased significantly in osppr11 mutants. The OsPPR11 is required for ndhA, and ycf3-1 introns splicing and interact with CRM family protein OsCAF2, suggesting that these two proteins may form splicing complexes to regulate group II introns splicing. Further analysis revealed that OsCAF2 interacts with OsPPR11 through the N-terminus. These results indicate that OsPPR11 is essential for chloroplast development and function by affecting group II intron splicing in rice.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by National Natural Science Foundation of China (Grant No. 32188102). The National Key R&D Program of China, Rice germplasm precisely evaluation for direct-breeding in east China (No.2021YFD1200503). The Agricultural Science and Technology Innovation Program. Key agricultural technology research projects (NK2022010302).

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  1. Qiang Zhang, Changzhao Chen and Yaliang Wang contributed equally to this work.

Authors and Affiliations

  1. State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, People’s Republic of China

    Qiang Zhang, Changzhao Chen, Yaliang Wang, Mengxing He, Zhiwen Li, Lan Shen, Qing Li, Li Zhu, Deyong Ren, Jiang Hu, Zhenyu Gao, Guangheng Zhang & Qian Qian

  2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China

    Mengxing He

  3. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310006, People’s Republic of China

    Zhiwen Li

  4. National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572000, People’s Republic of China

    Qian Qian

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  1. Qiang Zhang
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Contributions

QQ and QZ conceived and designed research. QZ, CCZ and YLW performed experiments. MXH and LLZ collected and analyzed the data. QZ and YLW wrote the manuscript. LS, QL, DYR, JH, LZ were provided useful suggestions during the course of study; GHZ and ZYG analyzed the data and review the manuscript. All authors read and approved the final manuscript.

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Correspondence to Qian Qian.

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Communicated by Li Tian.

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Zhang, Q., Chen, C., Wang, Y. et al. OsPPR11 encoding P-type PPR protein that affects group II intron splicing and chloroplast development. Plant Cell Rep 42, 421–431 (2023). https://doi.org/10.1007/s00299-022-02968-6

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