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EgMYB108 regulates very long-chain fatty acid (VLCFA) anabolism in the mesocarp of oil palm

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Abstract

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EgMYB108 regulates VLCFA anabolism in oil palm.

Very long-chain fatty acids (VLCFAs), which are fatty acids with more than 18 C, can not only be used as a form of triglyceride (TAG) but also provide precursors for the biosynthesis of cuticle wax, and they exist in plant epidermal cells in the form of wax in higher plants. However, which and how transcriptional factors (TFs) regulate this process is largely unknown in oil palm. In this study, a MYB transcription factor (EgMYB108) with high expression in the mesocarp of oil palm fruit was characterized. Overexpression of EgMYB108 promoted not only total lipid content but also VLCFA accumulation in oil palm embryoids. Subsequently, transient transformation in protoplasts and qRT–PCR analysis indicated that the EgKCS5 and EgLACS4 genes were significantly increased with the overexpression of EgMYB108. Furthermore, yeast one‑hybrid assays, dual-luciferase assays and EMSAs demonstrated that EgMYB108 binds to the promoters of EgKCS5 and EgLACS4 and regulates their transcription. Finally, EgMYB108 interacts with the promoters of EgLACS and EgKCS simultaneously and finally improves the VLCFA and total lipid contents; a pathway summarizing this interaction was depicted.. The results provide new insight into the mechanism by which EgMYB108 regulates lipid and VLCFA accumulation in oil palm.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

We are grateful to the following investigators for helping with the tissue culture and protoplast transformation: Ms. Qin Zhang and Yifei Wang at Hainan University.

Funding

This research was supported by the Hainan Provincial Natural Science Foundation of China (No. 2019CXTD397), the National Natural Science Foundation of China (NSFC) (No. 31660222) and National Key R&D Program of China (No. 2018YFD1000500).

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Author notes

  1. Xin Xu and Menghan Li these authors contributed equally to the article.

Authors and Affiliations

  1. Sanya Nanfan Research Institute, College of Tropical Crops, Hainan University, Sanya, 572025, Hainan, China

    Xin Xu, Menghan Li, Ji-xin Zou, Yu-sheng Zheng & Dong-dong Li

  2. Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, 571101, China

    Ji-xin Zou

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  1. Xin Xu
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Correspondence to Dong-dong Li.

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Communicated by Chun-Hai Dong.

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Xu, X., Li, M., Zou, Jx. et al. EgMYB108 regulates very long-chain fatty acid (VLCFA) anabolism in the mesocarp of oil palm. Plant Cell Rep 41, 1449–1460 (2022). https://doi.org/10.1007/s00299-022-02868-9

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  • DOI: https://doi.org/10.1007/s00299-022-02868-9

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