Abstract
Key message
Among the 113 lipases present in rice genome, bran and endosperm-specific lipases were identified and lipase activity for one of the selected lipase gene is demonstrated in yeast.
Abstract
Rice bran is nutritionally superior than endosperm as it has major reservoirs of various minerals, vitamins, essential mineral oils and other bioactive compounds, however it is often under-utilized as a food product due to bran instability after milling. Various hydrolytic enzymes, such as lipases, present in bran causes degradation of the lipids present and are responsible for the bran instability. Here, in this study, we have systematically analyzed the 113 lipase genes present in rice genome, and identified 21 seed-specific lipases. By analyzing the expression of these genes in different seed tissues during seed development, we have identified three bran-specific and three endosperm-specific lipases, and one lipase which expresses in both bran and endosperm tissues. Further analysis of these genes during seed maturation and seed germination revealed that their expression increases during seed maturation and decreases during seed germination. Finally, we have shown the lipase activity for one of the selected genes, LOC_Os05g30900, in heterologous system yeast. The bran-specific lipases identified in this study would be very valuable for engineering designer rice varieties having increased bran stability in post-milling.
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The funding was funded by DST-INSPIRE Faculty grant from Department of Science and Technology, Govt. of India (Grant no. DST/INSPIRE/04/2016/001118).
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HR conceived and designed the research. SB, SS, KS, MK and RKB performed the experiments. HS and RD contributed to reagents and analytic tools. HR and SB wrote the manuscript. All the authors read and edited the manuscript.
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Communicated by Hiroyasu Ebinuma.
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Fig S1.
Expression patterns of selected 21 lipase genes at Rice Genome Annotation Project database (A) and ePlant database (B). Given expression (FPKM) values are presented as a heatmap (PDF 374 KB)
Fig S2. Expression analysis of selected endosperm specific lipase genes during seed maturation and germination.
Q-PCR analysis of selected three lipases at (A) different seed maturation stages (given as days after fertilization) and (B) seed germination stages (given as days after imbibition). (PDF 50 KB)
Table S1.
Lipase motif containing lipases. (DOCX 27 KB)
Table S2.
Analysis of cis-elements in 1 Kb upstream promoter region of selected bran and endosperm specific genes. (XLSX 32 KB)
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Bansal, S., Sardar, S., Sinha, K. et al. Identification and molecular characterization of rice bran-specific lipases. Plant Cell Rep 40, 1215–1228 (2021). https://doi.org/10.1007/s00299-021-02714-4
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DOI: https://doi.org/10.1007/s00299-021-02714-4