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Evaluation of daidzein-loaded chitosan microcapsules for the colon cancer drug delivery: synthesis, characterization and release behaviour

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Abstract

The aim of the present study was to synthesize the chitosan-encapsulated daidzein (CED) and to evaluate its releasing behaviour in the different simulated gastrointestinal fluids. Moreover, the cytotoxic potential and antiangiogenic effects of CED were determined against human colon cancer cells and chorioallantoic blood vessels, respectively. The synthesized daidzein-loaded microcapsules possessed micrometre size, with good stability in colloidal dispersion with 64.3% entrapment efficiency. The release behaviour assay indicated the gradual release of daidzein in the simulated stomach and intestinal fluid (~ 38%; pH 1.2–4.5), while the substantial release was occurred in the simulated colonic fluid (79%; pH 6.8–7). The daidzein-loaded microcapsules inhibited the proliferation of human colorectal cancer cells (HT-29) with no prominent reduction in the viability of human dermal fibroblast (HDF). The expression analysis of caspase-3 gene together with flow cytometry results confirmed the apoptotic cells death in the HT-29 cells induced by daidzein-loaded microcapsules. Further, the daidzein-loaded microcapsules indicated the angiogenesis inhibitory activity. Consequently, these results revealed that microencapsulation of daidzein by chitosan could be a feasible approach for the delivery of daidzein, a soy-derived isoflavone for the colon cancer prevention and treatment.

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Acknowledgments

The authors would like to thank the Mashhad Branch, the Islamic Azad University of Mashhad and Arka biotechnology corporation for the grant and provided chemicals and laboratory facilities.

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Authors and Affiliations

  1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Robabbeh Sanatkar, Ghasem Rahimi Kalateh Shah Mohammad & Ehsan Karimi

  2. Department of Research and Development, Arka Biotechnology Corporation, Mashhad, Iran

    Ehsan Oskoueian

  3. Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Riau, Pekanbaru, Indonesia

    Rudi Hendra

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  1. Robabbeh Sanatkar
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Correspondence to Ehsan Karimi.

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Sanatkar, R., Rahimi Kalateh Shah Mohammad, G., Karimi, E. et al. Evaluation of daidzein-loaded chitosan microcapsules for the colon cancer drug delivery: synthesis, characterization and release behaviour. Polym. Bull. 79, 7391–7405 (2022). https://doi.org/10.1007/s00289-021-03853-0

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  • DOI: https://doi.org/10.1007/s00289-021-03853-0

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