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Controlled release in vitro of icariin from gelatin/hyaluronic acid composite microspheres

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Abstract

To fabricate a controlled release system for icariin for application to tissue engineering, gelatin/hyaluronic acid (Gel/HA) composite microspheres loaded icariin were prepared through emulsion-coagulation method using glutaraldehyde (GA) as cross-linker. The free Gel/HA and Gel/HA-icariin microspheres were characterized by Fourier transforms infrared (FTIR) and scanning electron microscopy (SEM). The influences of cross-linker content on the drug loaded and encapsulation efficiency of the Gel/HA microspheres were investigated, and the effects of cross-linker content, cross-linking time and loaded drug content on the release behavior of icariin in vitro were also studied. Moreover, the in vitro release kinetics of icariin from Gel/HA microspheres was analyzed. The results showed that both Gel/HA and Gel/HA-icariin microspheres are spherical with size ranged from 20 to 60 μm, and the Gel/HA-icariin microspheres showed rougher surface than Gel/HA microspheres. The loaded efficiency and the encapsulation efficiency decreased with increasing cross-linker content. The cumulative release of icariin from the Gel/HA microspheres decreased with increasing GA content and cross-linking time; however, the cumulative release increased with increasing icariin content in the microspheres. The results of icariin release kinetics from Gel/HA microspheres indicated Fickian diffusion. We concluded that it was feasible for Gel/HA to act as carriers of slow release of microspheres, and the release rate of icariin from the microspheres was adjustable.

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Acknowledgments

This work was financially supported by the National Natural Science Fund of China (Nos. 51273062, 51443002), IUC Program of Hunan Provincial Education Department (No. 15CY004) and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, People’s Republic of China

    Hua Yan, Zhihua Zhou, Qingquan Liu, Hu Zhou, Wennan Zeng, Lihua Liu, Baoli Ou, Siliang He & Huihua Huang

  2. Key Laboratory of Theoretical Organic Chemistry and Function Molecule of Ministry of Education, Hunan University of Science and Technology, Xiangtan, People’s Republic of China

    Zhihua Zhou

  3. Hunan Province College Key Laboratory of QSAR/QSPR, Hunan University of Science and Technology, Xiangtan, People’s Republic of China

    Zhihua Zhou

  4. Department of Orthopedics, the Second Xiangya Hospital, Central South University, Changsha, People’s Republic of China

    Tianlong Huang

  5. Department of Burns and Plastic Surgery, the Third Xiangya Hospital, Central South University, Changsha, People’s Republic of China

    Cheng Peng

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  1. Hua Yan
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  2. Zhihua Zhou
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  3. Tianlong Huang
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  4. Cheng Peng
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  8. Lihua Liu
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  10. Siliang He
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  11. Huihua Huang
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Correspondence to Zhihua Zhou.

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Yan, H., Zhou, Z., Huang, T. et al. Controlled release in vitro of icariin from gelatin/hyaluronic acid composite microspheres. Polym. Bull. 73, 1055–1066 (2016). https://doi.org/10.1007/s00289-015-1534-x

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  • DOI: https://doi.org/10.1007/s00289-015-1534-x

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