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Analysis of Salt Stress on Soil Microbial Community Composition and Its Correlation with Active Components in the Rhizosphere of Acanthopanax senticosus

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Abstract

Salt stress can adversely affect plant seed germination, growth and development, and eventually lead to slow growth and even death of plants. The purpose of this study was to investigate the effects of different concentrations of NaCl and Na2SO4 stress on the physicochemical properties, enzyme activities, rhizosphere microbial community and seven active components (l-phenylalanine, Protocatechuic acid, Eleutheroside B, Chlorogenic acid, Caffeic acid, Eleutheroside E, Isofraxidin) of Acanthopanax senticosus rhizosphere soil. Statistical analysis was used to explore the correlation between the rhizosphere ecological factors of Acanthopanax senticosus and its active components. Compared with Acanthopanax senticosus under NaCl stress, Na2SO4 generally had a greater effect on Acanthopanax senticosus, which reduced the richness of fungi in rhizosphere soil and adversely affected the content of multiple active components. Pearson analysis showed that pH, organic matter, ammonium nitrogen, available phosphorus, available potassium, catalase and urease were significantly correlated with active components such as Caffeic acid and Isofraxidin. There were 11 known bacterial genera, 12 unknown bacterial genera, 9 known fungal genera and 1 unknown fungal genus significantly associated with the active ingredient. Salt stress had great changes in the physicochemical properties, enzyme activities and microorganisms of the rhizosphere soil of Acanthopanax senticosus. In conclusion, different types and concentrations of salts had different effects on Acanthopanax senticosus, and the active components of Acanthopanax senticosus were regulated by rhizosphere soil ecological factors.

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Funding

This work was supported by grants from some project funds. Central level major increase and decrease project (2060302); Heilongjiang Natural Science Foundation joint guidance project (LH2020H093); “Heilongjiang University of Traditional Chinese Medicine outstanding young and middle-aged backbone teachers training support plan” project Scientific and Technological Innovation Leading Talents Program of the Corps (15041190011); Heilongjiang University of Traditional Chinese Medicine Graduate Innovative Research Project (2023yjscx046).

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  1. Wenhui Yuan and Shouyu Wang have contributed equally to this work and share first authorship.

Authors and Affiliations

  1. College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, China

    Wenhui Yuan, Shouyu Wang, Jingru Liang, Jiajie Shi, Yang Zhang & Changhong Ding

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  1. Wenhui Yuan
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  2. Shouyu Wang
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  3. Jingru Liang
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  5. Yang Zhang
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  6. Changhong Ding
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Contributions

All authors contributed to the study conception and design. Experimental inquiry and manuscript writing by Wenhui Yuan and Shouyu Wang. Data analysis and charting by Jingru Liang, Jiajie Shi and Yang Zhang. Project management and fund support by Changhong Ding. All authors commented on previous versions of the manuscript and all authors read and approved the final manuscript.

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Correspondence to Changhong Ding.

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Yuan, W., Wang, S., Liang, J. et al. Analysis of Salt Stress on Soil Microbial Community Composition and Its Correlation with Active Components in the Rhizosphere of Acanthopanax senticosus. Curr Microbiol 81, 160 (2024). https://doi.org/10.1007/s00284-024-03687-9

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