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Draconibacterium aestuarii sp.nov., a Glycolipid-Producing Bacterium Isolated from Tidal Flat Sediment and Emended Description of the Genus Draconibacterium

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Abstract

A facultatively anaerobic, Gram-negative, curved rod-shaped bacterium (4.0–17.0 μm long, 0.6–0.9 μm wide), designated Z1-6T, was obtained from tidal flat sediment collected from YueAo village in Zhoushan, Zhejiang, People’s Republic of China. Strain Z1-6T occurred at 15–45 °C (optimum 28–32 °C), pH 6.0–9.0 (optimum 7.0–7.5), and in the presence of 1–5% (w/v) NaCl (optimum 1–2%). The strain contained iso-C15:0 and antesio-C15:0 as the major fatty acids. An unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone. The polar lipids included phosphatidylethanolamine (PE), one aminophospholipid (APL), two phospholipids (PL1 and PL2), three glycolipids (GL1, GL2, and GL3), and two unidentified lipids (L1 and L2). The genomic DNA G+C content of strain Z1-6T was 39.2%, and the genome size was 6.4 Mb. The strain showed the highest average nucleotide identity (ANI) value of 73.5–74.6%, digital DNA–DNA hybridization (dDDH) value of 19.3–20%, average amino acid identity (AAI) value of 72.0–73.1% with the members of genus Draconibacterium. Phylogenetic analysis based on 16S rRNA gene sequences and genome revealed that strain Z1-6T formed a distinct branch in the clade of the genus Draconibacterium. Based on the phenotypic, phylogenetic, chemotaxonomic analyses and genomic data, strain Z1-6T represents a novel species of the genus Draconibacterium, for which the name Draconibacterium aestuarii sp. nov. (The type strain Z1-6T = MCCC 1K07533T = KCTC 92310T) is proposed.

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Funding

This research was funded by grants from “Pioneer” and “Leading Goose” R&D Program of Zhejiang (No.2024C03123), Key R&D Program of Zhejiang (2022C0310), National Natural Science Foundation of China (no.82002208), National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700) and National Science and Technology Fundamental Resources Investigation Program of China (2021FY100900).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Jia-Yan Wang, Wei-Yu Pan & Min Wu

  2. Chu Kochen Honors College, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Sha-Sha Qiu

  3. Trend Biotech Co., Ltd., Hangzhou, 310012, People’s Republic of China

    Yue Su & Wen-Wu Zhang

  4. Department of Microbiology, University of Georgia, Athens, GA, USA

    Tao Wang

  5. Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, People’s Republic of China

    Ge-Yi Fu

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  1. Jia-Yan Wang
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  3. Yue Su
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Contributions

MW and GYF designed the experiments and guided the manuscript writing. JYW was responsible for the major experiments, data analysis and preparation of manuscripts. SSQ and WYP assisted in enzymatic experiments. TW assisted in the determination of fatty acids and polar lipids experiment. WWZ and YS are responsible for sample collection. All authors read and approved the manuscript.

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Correspondence to Ge-Yi Fu or Min Wu.

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Repositories The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Z1-6T is OP882736. The GenBank/EMBL/DDBJ accession number for draft genome sequence of strain Z1-6T is JAPOHD000000000.

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Wang, JY., Qiu, SS., Su, Y. et al. Draconibacterium aestuarii sp.nov., a Glycolipid-Producing Bacterium Isolated from Tidal Flat Sediment and Emended Description of the Genus Draconibacterium. Curr Microbiol 81, 162 (2024). https://doi.org/10.1007/s00284-024-03682-0

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