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Microbulbifer litoralis sp. nov., Isolated from Seashore of Weizhou Island

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Abstract

A bacterium designated GXH0434T was isolated from sea shore samples collected from Weizhou Island, Beihai, Guangxi, China. The organism is motile, strictly aerobic, and possesses a rod–coccus cell cycle in association with the growth phase. It can grow at 15–45 °C (optimum 37 °C), at pH 6.0–11.0 (optimum 6.0), and at 0–20% (w/v) NaCl (optimum 5.0–8.0%). The strain is positive for peroxidase and oxidase activity, negative for Voges-Proskauer test, can hydrolyze Tween 20, Tween 60, Tween 80, casein, and is able to produce siderophore and has the function of nitrogen fixation. Molecular phylogenetic analysis based on 16S rRNA gene sequences indicated that GXH0434T was most closely related to Microbulbifer halophilus KCTC 12848T with the similarity of 97.2%, followed by Microbulbifer chitinilyticus JCM 16148T (97.1%) and Microbulbifer taiwanensis LMG 26125T (96.5%). The digital DNA-DNA hybridization and the average nucleotide identity values between GXH0434T and Microbulbifer halophilus KCTC 12848T were 28.90% and 83.38%, respectively, which were below thresholds of species delineation. The genomic DNA G+C content of the strain was 61.9%. The major fatty acids were iso-C15:0, C16:0, iso-C11:0 3-OH, iso-C11:0 and Summed features 8 (C18:0 ω7c and/or C18:0 ω6c). The major polar lipids detected in GXH0434T were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC). The major respiratory quinone was ubiquinone Q-8. Based on the above polyphasic classification indicated strain GXH0434T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer litoralis sp. nov. is proposed. The type strain is GXH0434T (= MCCC 1K07158T = KCTC 92169T).

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Acknowledgements

We thank the Korean Collection of Type Cultures (KCTC) for providing strain Microbulbifer halophilus KCTC 12848T, and Marine Culture Collection of China (MCCC) and Korean Collection for Type Cultures (KCTC) for strain deposited, as well as members of the Guangxi Key Laboratory of Polysaccharide Materials and Modifications and Yunnan Institute of Microbiology of Yunnan University for helpful discussions.

Funding

This research was funded by the Science and Technology Major Project of Guangxi (AA18242026) and Guangxi University Young and Mid-aged Teachers’ Basic Scientific Research Ability Improvement Project of Universities in Guangxi, China (2021KY0175).

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Authors and Affiliations

  1. School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning, 530008, People’s Republic of China

    Ying Huang, Aolin Zhao, Fang Wang, Hongcun Liu & Mingguo Jiang

  2. College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Yu Jiang

  3. School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning, 530008, People’s Republic of China

    Ying liu

  4. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Xuemei Chen & Yi Jiang

  5. Guangxi Lvyounong Biological Technology Co., Ltd, Nanning, 530000, People’s Republic of China

    Wenshan Huang

  6. Department of Marine Science, Universitas Padjadjaran, Jatinangor, Indonesia

    Yudi N. Ihsan

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Contributions

MJ and YJ designed the study; YH, YJ, AZ and XC performed research; YL, FW and HL analyzed data. YNI and WH resource; YH wrote the manuscript. All of the authors contributed to the manuscript revision process and read and approved the submitted version.

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Correspondence to Mingguo Jiang or Yi Jiang.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and the genome sequences of strain GXH0434T are OL336515 and JALKCV000000000, respectively. Strain GXH0434T has been deposited in Marine Culture Collection of China (MCCC) and Korean Collection for Type Cultures (KCTC), the preservation number are MCCC 1K07158 and KCTC 92169, respectively.

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Huang, Y., Jiang, Y., Zhao, A. et al. Microbulbifer litoralis sp. nov., Isolated from Seashore of Weizhou Island. Curr Microbiol 81, 105 (2024). https://doi.org/10.1007/s00284-023-03594-5

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