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Surface Disinfection and Sanitizing Action of the Alcohol-Free Essential Oil-based Green Formulation

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Abstract

The surface disinfectant property of a prepared formulation using potential and effective EO (Murraya koenigii), phytochemical (Geraniol), and an amino acid epsilon-l-Poly-Lysine (ɛ-PL) is examined in this present study. To investigate its potential as a surface disinfectant (SD) different tests using multiple bacterial strains were conducted. All tested bacterial strains were inhibited by the SD treatments, with a MIC range of (0.78–3.12%) v/v. Notably, Staphylococcus sp. was found to be more susceptible to the treatment than its gram-negative counterparts. In the test, sterile stainless-steel surfaces were used and externally contaminated with Escherichia sp. Cleaning the surface with the prepared formulation was more effective than the equal concentration of vinegar in terms of bacterial growth reduction. Vinegar was used as a mother solvent in the preparation of the SD due to its proven antibacterial effect. It is worth mentioning, this formulation is also proven to be effective on biofilm-embedded bacterial cells of Pseudomonas aeruginosa PAO1 as found in epifluorescence microscopy staining. Even though the impact of each constituent needs to be further explored, the effectiveness of this formulation may encourage large farms to seek out alternatives that are more environmentally friendly, safe, and effective than conventional products.

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Data Availability

The datasets used and/or analysed during the current study will be available from the corresponding author on reasonable request at raivittal@gmail.com.

References

  1. Kamala K, Kumar VP (2018) Food products and food contamination. Microb Contam Food Degrad. https://doi.org/10.1016/B978-0-12-811515-2.00001-9

    Article  Google Scholar 

  2. Usfar AA, Iswarawanti DN, Davelyna D, Dillon D (2010) Food and personal hygiene perceptions and practices among caregivers whose children have Diarrhea: a qualitative study of Urban mothers in Tangerang, Indonesia. J Nutr Educ Behav 42:33–40. https://doi.org/10.1016/J.JNEB.2009.03.003

    Article  PubMed  Google Scholar 

  3. Ismaïl R, Aviat F, Michel V et al (2013) Methods for recovering microorganisms from solid surfaces used in the food industry: a review of the literature. Int J Environ Res Public Heal 2013:6169–6183. https://doi.org/10.3390/IJERPH10116169

    Article  Google Scholar 

  4. Allen VM, Bull SA, Corry JEL et al (2007) Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonisation. Int J Food Microbiol 113:54–61. https://doi.org/10.1016/J.IJFOODMICRO.2006.07.011

    Article  CAS  PubMed  Google Scholar 

  5. Tomadoni B, Cassani L, Del M et al (2019) Natural antimicrobials combined with ultrasound treatments to enhance quality parameters and safety of unpasteurized strawberry juice. Int J Fruit Sci. https://doi.org/10.1080/15538362.2019.1709115

    Article  Google Scholar 

  6. Co GILL, Mcginnis JC, Bryant J (2001) Contamination of beef chucks with Escherichia coli during carcass breaking. J Food Prot 64:1824–1827. https://doi.org/10.4315/0362-028X-64.11.1824

    Article  Google Scholar 

  7. Banatvala N, Magnano AR, Cartter ML et al (1996) Meat grinders and molecular epidemiology: two supermarket outbreaks of Escherichia coli 0157:H7 infection. J Infect Dis 173:480–483. https://doi.org/10.1093/INFDIS/173.2.480

    Article  CAS  PubMed  Google Scholar 

  8. Armstrong GL, Hollingsworth J, Morris JG (1996) Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiol Rev 18:29

    Article  CAS  PubMed  Google Scholar 

  9. Rangel JM, Sparling PH, Crowe C et al (2005) Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982–2002. Emerg Infect Dis 11:603. https://doi.org/10.3201/EID1104.040739

    Article  PubMed  PubMed Central  Google Scholar 

  10. Beuchat LR (2002) Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes Infect 4:413–423. https://doi.org/10.1016/S1286-4579(02)01555-1

    Article  PubMed  Google Scholar 

  11. Ye K, Wang J, Han Y et al (2019) Investigation on microbial contamination in the cold storage room of domestic refrigerators. Food Control 99:64–67. https://doi.org/10.1016/J.FOODCONT.2018.12.022

    Article  CAS  Google Scholar 

  12. Söderqvist K, Ahmed Osman O, Wolff C et al (2017) Emerging microbiota during cold storage and temperature abuse of ready-to-eat salad. Infect Ecol Epidemiol. https://doi.org/10.1080/20008686.2017.1328963

    Article  PubMed  PubMed Central  Google Scholar 

  13. Maurya A, Prasad J, Das S, Dwivedy AK (2021) Essential oils and their application in food safety. Front Sustain Food Syst. https://doi.org/10.3389/FSUFS.2021.653420

    Article  Google Scholar 

  14. Gopal J, Anthonydhason V, Muthu M et al (2017) Authenticating apple cider vinegar’s home remedy claims: antibacterial, antifungal, antiviral properties and cytotoxicity aspect. Nat Prod Res 33:906–910. https://doi.org/10.1080/14786419.2017.1413567

    Article  CAS  PubMed  Google Scholar 

  15. Gaber SN, Bassyouni RH, Masoud M, Ahmed FA (2020) Promising anti-microbial effect of apple vinegar as a natural decolonizing agent in healthcare workers. Alexandria J Med 56:73–80. https://doi.org/10.1080/20905068.2020.1769391

    Article  CAS  Google Scholar 

  16. Lucera A, Costa C, Conte A, Del Nobile MA (2012) Food applications of natural antimicrobial compounds. Front Microbiol 3:287. https://doi.org/10.3389/FMICB.2012.00287/BIBTEX

    Article  PubMed  PubMed Central  Google Scholar 

  17. Kayumov AR, Nureeva AA, Trizna EY et al (2015) New derivatives of pyridoxine exhibit high antibacterial activity against biofilm-embedded staphylococcus cells. Biomed Res Int. https://doi.org/10.1155/2015/890968

    Article  PubMed  PubMed Central  Google Scholar 

  18. Zanfardino A, Criscuolo G, Di Luccia B et al (2017) Identification of a new small bioactive peptide from Lactobacillus gasseri supernatant. Benef Microbes 8:133–141. https://doi.org/10.3920/BM2016.0098

    Article  CAS  PubMed  Google Scholar 

  19. Lin CM, Sheu SR, Hsu SC, Tsai YH (2010) Determination of bactericidal efficacy of essential oil extracted from orange peel on the food contact surfaces. Food Control 21:1710–1715. https://doi.org/10.1016/J.FOODCONT.2010.06.008

    Article  CAS  Google Scholar 

  20. Rajendran MP, Pallaiyan BB, Selvaraj N (2014) Chemical composition, antibacterial and antioxidant profile of essential oil from Murraya koenigii (L.) leaves. Avicenna J Phytomed 4:200

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Chatterjee B, Aswathanarayan JB, Vittal RR (2022) Application of geraniol-chitosan blend film with quorum sensing inhibitory activity as packaging materials for biofilm control in fresh fruit and vegetable. J Packag Technol Res 2022:1–14. https://doi.org/10.1007/S41783-022-00133-8

    Article  Google Scholar 

  22. Hyldgaard M, Mygind T, Vad BS et al (2014) The antimicrobial mechanism of action of epsilon-poly-l-lysine. Appl Environ Microbiol 80:7758–7770. https://doi.org/10.1128/AEM.02204-14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Wang G, Jia S, Wang T et al (2011) Effect of ferrous ion on ε-Poly-l-lysine biosynthesis by Streptomyces diastatochromogenes CGMCC3145. Curr Microbiol 62:1062–1067. https://doi.org/10.1007/S00284-010-9828-6/FIGURES/4

    Article  CAS  PubMed  Google Scholar 

  24. Yoshida T, Nagasawa T (2003) ε-poly-l-lysine: Microbial production, biodegradation and application potential. Appl Microbiol Biotechnol 62:21–26. https://doi.org/10.1007/S00253-003-1312-9

    Article  CAS  PubMed  Google Scholar 

  25. Chouegouong MT, Majoumouo MS, Menkem EZ et al (2021) Ethnopharmacological survey and antibacterial activity of medicinal plant extracts used against bacterial enteritis in rabbits. Adv Tradit Med. https://doi.org/10.1007/S13596-021-00615-1/TABLES/5

    Article  Google Scholar 

  26. Mahmoudzadeh M, Hosseini H, Mahmoudzadeh L, Mazaheri Nezhad Fard R (2020) Comparative effects of Carum copticum essential oil on bacterial growth and Shiga-toxin gene expression of Escherichia coli O157:H7 at abused refrigerated temperatures. Curr Microbiol 77:1660–1666. https://doi.org/10.1007/S00284-020-01987-4/FIGURES/4

    Article  CAS  PubMed  Google Scholar 

  27. Bai AJ, Vittal RR (2014) Quorum sensing inhibitory and anti-biofilm activity of essential oils and their in vivo efficacy in food systems. Food Biotechnol 28:269–292

    Article  CAS  Google Scholar 

  28. Sankar Ganesh P, Rai Vittal R (2015) In vitro antibiofilm activity of Murraya koenigii essential oil extracted using supercritical fluid CO2 method against Pseudomonas aeruginosa PAO1. Nat Prod Res 29:2295–2298. https://doi.org/10.1080/14786419.2015.1004673

    Article  CAS  PubMed  Google Scholar 

  29. Cazella LN, Glamoclija J, Soković M et al (2019) Antimicrobial activity of essential oil of Baccharis dracunculifolia DC (Asteraceae) aerial parts at flowering period. Front Plant Sci 10:27. https://doi.org/10.3389/FPLS.2019.00027/BIBTEX

    Article  PubMed  PubMed Central  Google Scholar 

  30. Herman A, Tambor K, Herman A (2016) Linalool affects the antimicrobial efficacy of essential oils. Curr Microbiol 72:165–172. https://doi.org/10.1007/S00284-015-0933-4/TABLES/2

    Article  CAS  PubMed  Google Scholar 

  31. Guo F, Chen Q, Liang Q et al (2021) Antimicrobial activity and proposed action mechanism of linalool against Pseudomonas fluorescens. Front Microbiol 12:562094. https://doi.org/10.3389/FMICB.2021.562094

    Article  PubMed  PubMed Central  Google Scholar 

  32. Lira MHP, Junior FPA, Moraes GFQ et al (2020) Antimicrobial activity of geraniol: an integrative review. J Essent Oil Res 32:187–197. https://doi.org/10.1080/10412905.2020.1745697

    Article  CAS  Google Scholar 

  33. Nazzaro F, Fratianni F, Martino L et al (2013) Effect of essential oils on pathogenic bacteria. Pharmaceuticals 6:1451–1474. https://doi.org/10.3390/PH6121451

    Article  PubMed  PubMed Central  Google Scholar 

  34. Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308–316. https://doi.org/10.1046/J.1365-2672.2000.00969.X

    Article  CAS  PubMed  Google Scholar 

  35. Arman M, Pirian K, Alinaghizadeh M et al (2021) Study of compounds, cytotoxicity and biological activities of essential oil of Satureja rechingeri Jamzad. Adv Tradit Med. https://doi.org/10.1007/S13596-021-00596-1/FIGURES/4

    Article  Google Scholar 

  36. Amaral VCS, Santos PR, da Silva AF et al (2015) Effect of carvacrol and thymol on Salmonella spp. biofilms on polypropylene. Int J Food Sci Technol 50:2639–2643. https://doi.org/10.1111/IJFS.12934

    Article  CAS  Google Scholar 

  37. Raffaella C, Casettari L, Fagioli L et al (2017) Activity of essential oil-based microemulsions against Staphylococcus aureus biofilms developed on stainless steel surface in different culture media and growth conditions. Int J Food Microbiol 241:132–140. https://doi.org/10.1016/J.IJFOODMICRO.2016.10.021

    Article  CAS  Google Scholar 

  38. Cui H, Li W, Li C, Lin L (2016) Synergistic effect between Helichrysum italicum essential oil and cold nitrogen plasma against Staphylococcus aureus biofilms on different food-contact surfaces. Int J Food Sci Technol 51:2493–2501. https://doi.org/10.1111/IJFS.13231

    Article  CAS  Google Scholar 

  39. Nguyen HDN, Yuk HG (2013) Changes in resistance of Salmonella Typhimurium biofilms formed under various conditions to industrial sanitizers. Food Control 29:236–240. https://doi.org/10.1016/J.FOODCONT.2012.06.006

    Article  CAS  Google Scholar 

  40. Klein G, Rüben C, Upmann M (2013) Antimicrobial activity of essential oil components against potential food spoilage microorganisms. Curr Microbiol 67:200–208. https://doi.org/10.1007/S00284-013-0354-1/TABLES/5

    Article  CAS  PubMed  Google Scholar 

  41. Kerekes EB, Vidács A, Takó M et al (2019) Anti-biofilm effect of selected essential oils and main components on mono- and polymicrobic bacterial cultures. Microorganisms. https://doi.org/10.3390/MICROORGANISMS7090345

    Article  PubMed  PubMed Central  Google Scholar 

  42. Guimarães AC, Meireles LM, Lemos MF et al (2019) Antibacterial activity of terpenes and terpenoids present in essential oils. Molecules. https://doi.org/10.3390/MOLECULES24132471

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors would like to acknowledge Vijnana Bhavan, for providing the instrumental facility of Advanced Fluorescence Microscopy at the Institute of Excellence (IOE), University of Mysore, Mysore, India.

Funding

This present research work was funded by DRDO, New Delhi, India (Project No ERIP/ER/201611002/M/01/1672 dated 22nd June 2017).

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  1. Department of Studies in Microbiology, University of Mysore, Mysore, Karnataka, 570006, India

    Boudhyayan Chatterjee & Ravishankar Rai Vittal

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  1. Boudhyayan Chatterjee
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Contributions

BC: designed and conceptualized the experiments, performed and analysed data, and prepared the draft of the manuscript. RRV: conceptualized the study, supervised the study, read and edited the manuscript.

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Correspondence to Ravishankar Rai Vittal.

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Chatterjee, B., Vittal, R.R. Surface Disinfection and Sanitizing Action of the Alcohol-Free Essential Oil-based Green Formulation. Curr Microbiol 80, 170 (2023). https://doi.org/10.1007/s00284-023-03265-5

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