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Isolation of Alpha Amylase-Producing Bacteria from Local Region of Ambala and Production of Amylase Under Optimized Factors Using Solid-State Fermentation

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Abstract

Enzymes are one of the most significant products produced primarily from microbial sources for human requirements. The purpose of this work was to isolate, screen, and optimize enzyme production under solid-state fermentation. In the present study, amylase-producing bacteria were isolated from the local region of Ambala. A total of six samples were taken, out of which 14 isolates were isolated, among which seven isolates were found to be amylase producing. Highest amylase yield was obtained from isolate A11, further studied for the production of amylase under solid substrate fermentation (SSF), and also optimized the conditions for increased production of amylase. The molecular and biochemical characterization confirmed it as a strain of Alkalihalobacillus clausii. It was observed that growth parameters showed a profound effect on the production. The bacterium produces ample amount (7.3 × 103 IU/g) of alpha amylase using wheat bran using OVAT (one variable at a time) approach. Further using RSM (Response Surface Methodology) resulted in 3.78-fold increase in alpha amylase production, i.e., 27.57 × 103 IU/g.

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Acknowledgements

The author acknowledges the Chandigarh University for providing facilities.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or non-profit sectors.

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Authors and Affiliations

  1. University Institute of Biotechnology, Chandigarh University, Mohali, Punjab, 140413, India

    Anuradha Mittal, Mahavir Joshi & Vagish Dwibedi

  2. School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy, DIT University, Dehradun, Uttarakhand, 248009, India

    Santosh Kumar Rath

  3. Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India

    Davinder Singh

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  1. Anuradha Mittal
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  2. Mahavir Joshi
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  3. Santosh Kumar Rath
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  4. Davinder Singh
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Contributions

AM: Conceived the idea, designed the experiments, and carried out the experiments and wrote the manuscript. MJ: Carried out the experiments and wrote the manuscript. SKR: Data creation, Software, Formal analysis, and Review and editing. DS: Investigation, Formal analysis, and Review and editing. VD: Conceptualization, Methodology, Validation, Project Administration, and Review and editing.

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Correspondence to Vagish Dwibedi.

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Mittal, A., Joshi, M., Rath, S.K. et al. Isolation of Alpha Amylase-Producing Bacteria from Local Region of Ambala and Production of Amylase Under Optimized Factors Using Solid-State Fermentation. Curr Microbiol 79, 375 (2022). https://doi.org/10.1007/s00284-022-03081-3

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