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Novel Phocaeicola Strain Ameliorates Dextran Sulfate Sodium-induced Colitis in Mice

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Abstract

Previously, we isolated a novel Phocaeicola strain, Phocaeicola faecalis FXJYN30E22, from the feces of a healthy human from China. Metagenomic analysis revealed that the distribution of FXJYN30E22 differed in the intestinal tract of different hosts. We aimed to determine whether FXJYN30E22 protects against ulcerative colitis by employing a mouse model. In this study, dextran sulfate sodium was used to construct the UC model. The disease activity index, colon length, body weight changes, and histological scores were used as the pathological indicators to assess the anti-inflammatory effect of P. faecalis FXJYN30E22. Further, cytokine levels, tight junction mRNA expression levels, and short-chain fatty acid (SCFA) concentrations were also analyzed. Phocaeicola faecalis FXJYN30E22 could reduce the DSS-induced increase in DAI score, and enhance the colon length and body weight. Phocaeicola faecalis FXJYN30E22 could enhance TJ protein concentration and modulate the level of cytokines to reach levels close to those of the control group. FXJYN30E22 could also upregulate the concentrations of SCFA, which include acetate and butyrate. Based on the correlation analysis, four factors, including interleukin (IL)-6, IL-10, IL-1β levels, and propionate concentration, were related to the protective roles of FXJYN30E22 in UC mice to different degrees. According to an analysis of the genomic information, the potential protective effects of strain FXJYN30E22 may be associated with the secretion of SCFA by specific genes. These findings suggest that oral P. faecalis FXJYN30E22 could help maintain the epithelial barrier by regulating cytokine levels and secreting SCFA.

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Funding

This work was supported by the National Natural Science Foundation of China Key Program (31772090, 32001665, 31820103010, U1903205), Natural Science Foundation of Jiangsu Province (BE2021623), the Key Scientific and Technological Research Projects (2018AB010), the Major Project of Wuxi Science and Technology Bureau (Y20212007), and the Wuxi Double-Hundred Talent Fund Project (BJ2020082) and the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.

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Author notes

  1. Zhen Sun and Xinye Jiang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, No 1800 Lihu Avenue, Binhu District, Wuxi, 214122, Jiangsu, People’s Republic of China

    Zhen Sun, Botao Wang, Fengwei Tian & Leilei Yu

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Zhen Sun, Fengwei Tian & Leilei Yu

  3. Bloomage Biotechnology CO, LTD, Jinan, 250000, Shandong, China

    Botao Wang

  4. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Fengwei Tian

  5. Department of Child Health Care, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214002, China

    Xinye Jiang & Heng Zhang

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Contributions

ZS: Data curation; writing—original draft; conceived and designed the experiments. XJ: Data curation; writing—original draft. BW and FT: Data curation. HZ: Data curation. LY: Supervision; writing—review & editing; conceived and designed the experiments.

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Correspondence to Heng Zhang or Leilei Yu.

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The study protocol was approved by the Committee of Ethics of Jiangnan University, China (JN. No. 20201230c0400203[390]). All of the required procedures involving the use and care of animals for experiments were conducted according to the guidelines of the European Community (Directive 2010/63/EU).

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284_2022_3054_MOESM4_ESM.tif

Supplementary file4 (TIF 2019 kb) Fig. S1 Effect of P. faecalis FXJYN30E22 on the concentration of SCFA in cecal contents. Note: Statistical significance among different groups compared with the DSS group was determined.

284_2022_3054_MOESM5_ESM.tif

Supplementary file5 (TIF 4483 kb) Fig. S2 Morphology of P. faecalis FXJYN30E22 and B. fragilis NCTC9343 (A) hemolysis of B. fragilis NCTC9343; (B) hemolysis of P. faecalis FXJYN30E22; (C) motility of B. fragilis NCTC9343; and (D) motility of P. faecalis FXJYN30E22.

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Sun, Z., Jiang, X., Wang, B. et al. Novel Phocaeicola Strain Ameliorates Dextran Sulfate Sodium-induced Colitis in Mice. Curr Microbiol 79, 393 (2022). https://doi.org/10.1007/s00284-022-03054-6

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