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Description of Nocardioides jiangsuensis sp. nov., and Proposal for Reclassification of the Genus Marmoricola as Nocardioides

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Abstract

A Gram-staining-positive, non-motile, aerobic, spherical actinobacterium, designated WL0053T, was isolated from the coastal sediment of Nantong City, Jiangsu Province, China. The 16S rRNA gene sequence of strain WL0053T exhibited the highest similarities to Nocardioides mesophilus MSL-22T (98.0%), N. massiliensis GD12T (97.8%), Marmoricola bigeumensis MSL-05T (97.6%), and N. jensenii DSM 20641T (97.3%). The polyphasic taxonomic approach was used for the identification of strain WL0053T. This strain formed white, round, and smooth colonies and grew in the presence of 0–18% (w/v) NaCl (optimum, 0–4.0%), at pH 6.0–9.0 (optimum, pH 7.0) and at 20–37 °C (optimum, 28 °C). The main cellular fatty acids comprised of C17:1 ω8c, C18:1 ω9c, and iso-C16:0. The genomic DNA G + C content was 71.9%. The predominant quinone was MK-8(H4), and the major polar lipid consisted of phosphatidylcholine, glycolipid, phosphatidylethanolamine, and two unidentified phospholipids. Phylogenetic trees of 16S rRNA gene and bac120 gene set indicted that strain WL0053T was closely related to the species N. iriomotensis and N. mesophilus, while these two species clustered in a separate clade together with M. caldifontis YIM 730233T in the bac120 tree. Combined with the analysis of average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA–DNA hybridization (dDDH), it can be considered that the strain WL0053T is a new member of the genus Nocardioides and is proposed to be named as Nocardioides jiangsuensis sp. nov.. The type strain is WL0053T (=MCCC 1K05897T=JCM 34671T=GDMCC 4.192T). Furthermore, based on the fact that the genera Nocardioides and Marmoricola both appeared polyphyletic with no significant difference on phenotypic and chemotaxonomic traits, we proposed to reclassify the genus Marmoricola as Nocardioides.

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Data Availability

All of the data supporting the conclusions of this article are included within the article and its additional files. The genome datasets and the 16S rRNA gene sequence of Nocardioides jiangsuensis WL0053T generated during the current study are available in the GenBank repository under accession number JAIEZQ000000000 and OK473545, respectively. Other datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

Code Availability

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Acknowledgements

We are grateful to Professor Aharon Oren (The Hebrew University of Jerusalem, Israel) and Professor Wen-Jun Li (Sun Yat-Sen University, China) for the Latin construction of the taxa names.

Funding

This research was supported by the National Natural Science Foundation of China (No. 31900001), the China Postdoctoral Science Foundation (2020M671312) and the Fundamental Research Funds for the Central Universities (B210202140).

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Authors and Affiliations

  1. Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, People’s Republic of China

    Lu Wang, Hua-Peng Xue, Dao-Feng Zhang, Jian-Ke Huang, Chuang Liu & Ai Hua Zhang

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Contributions

D-FZ and LW designed research and project outline. LW and D-FZ performed isolation, deposition, and polyphasic taxonomy. D-FZ, LW, and H-PX performed genome analysis. LW, D-FZ, and AZ drafted the manuscript. J-KH and CL revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dao-Feng Zhang or Ai Hua Zhang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Declaration of Deposition in Repositories

The type strain Nocardioides jiangsuensis WL0053T had been deposited in Marine Culture Collection of China (MCCC), Japan Collection of Microorganisms (JCM), and Guangdong Microbial Culture Collection Center (GDMCC).

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Wang, L., Xue, HP., Zhang, DF. et al. Description of Nocardioides jiangsuensis sp. nov., and Proposal for Reclassification of the Genus Marmoricola as Nocardioides. Curr Microbiol 80, 60 (2023). https://doi.org/10.1007/s00284-022-02977-4

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