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Characterization of Sequence Types and Mechanisms of Resistance to Tigecycline Among Acinetobacter baumannii Isolated from Children

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Abstract

The present study aimed to investigate the mechanisms of resistance to tigecycline and to determine sequence types of Acinetobacter baumannii isolates recovered from children, using the Multilocus Sequence Typing (MLST). A total of 74 A. baumannii isolates were recovered from patients at one of the children’s hospital in Tehran, Iran. Antimicrobial susceptibility testing of the isolates was performed for different classes of antibiotics and minimum inhibitory concentrations of colistin and tigecycline were determined using broth microdilution method and E-test strips, respectively. The presence of ISAba1, AbaR, tet(39), and tetX and the expressions of adeB, adeG, and adeJ efflux pump genes were measured using Polymerase Chain Reaction (PCR) and quantitative real-time PCR (RT-PCR), respectively. The diversity of mutations across the regulatory genes of RND efflux pumps (adeRS, adeL, and adeN) and trm gene were determined using their PCR amplification and DNA sequencing in tigecycline-resistant isolates. In addition, STs of tigecycline-resistant isolates were determined using MLST method. Three A. baumannii isolates were resistant to tigecycline. Several amino acid substitutions were identified in AdeRS, AdeN, and Trm but no alteration was found in AdeL. Nevertheless, adeB, adeG, and adeJ overexpression were observed in 1, 2, and 1 isolates, respectively. The tigecycline-resistant isolates belonged to ST1720 and ST2285. This is the first study reporting on ST2285 in A. baumannii populations. Among 74 isolates, two tigecycline susceptible isolates carried tet(39) gene but no tetX gene was detected. We concluded that mutations in regulatory genes of RND efflux pumps and the trm gene may play some important role in A. baumannii resistance to tigecycline.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This article has been based on a Ph.D dissertation by Razieh Dehbanipour at the School of Medicine, Shahid Beheshti University of Medical Sciences (Registration No. 515).

Funding

The current study was supported by Shahid Beheshti University of Medical Sciences, Tehran, Iran (Grant Number 21038).

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Authors and Affiliations

  1. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zohreh Ghalavand

  2. Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zohreh Ghalavand, Gita Eslami, Ali Hashemi, Mehrzad Sadredinamin, Neda Yousefi & Razieh Dehbanipour

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Contributions

RD was involved in sample collection, study concept and design, laboratory research, interpretation of the data, and manuscript writing and reviewing. GE was involved in project supervision and manuscript reviewing. ZG was involved in the concept and design of the study, interpretation, and critical revision of the paper. MS and NY were responsible for some laboratory research, and AH was involved in data analysis and interpretation. All authors read and approved the final version of the manuscript.

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Correspondence to Razieh Dehbanipour.

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This study was approved by the local ethics committee of Shahid Beheshti University of Medical Sciences (IR.SBMU. RETEC.REC.1399.862).

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Consent to participate is required: research is carried out on clinical isolates recovered from hospitalized children.

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Ghalavand, Z., Eslami, G., Hashemi, A. et al. Characterization of Sequence Types and Mechanisms of Resistance to Tigecycline Among Acinetobacter baumannii Isolated from Children. Curr Microbiol 79, 285 (2022). https://doi.org/10.1007/s00284-022-02976-5

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