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Oceanobacter mangrovi Sp. Nov., a Novel Poly-β-hydroxybutyrate Accumulating Bacterium Isolated from Mangrove Sediment

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Abstract

A Gram-stain-negative, rod-shaped, motile, mesophilic, and aerobic bacterial strain, designated SM2-42 T was isolated from a mangrove sediment. Catalase activity and oxidase activity were positive. Growth was observed at 20 °C–40 °C, pH 6.0–8.0, and in the presence of 0.5–5.0% NaCl. Cells of strain SM2-42 T contained poly-β-hydroxybutyrate granules. The 16S rRNA gene of strain SM2-42 T had maximum sequence similarity with Oceanobacter kriegii 197 T of 97.1%. Phylogenetic analysis based on 16S rRNA gene sequence and 120 conserved concatenated proteins indicated that strain SM2-42 T was affiliated to the genus Oceanobacter and formed a monophyletic branch with O. kriegii 197 T. The average nucleotide identity and digital DNA-DNA hybridization values between strain SM2-42 T and O. kriegii 197 T were 76.43% and 21.60%, respectively. The major isoprenoid quinone was Q-8. The major fatty acids (> 10%) comprised C16:0, summed feature 8 (C18:1ω7c and C18:1 ω6c), C18:0, and summed feature 3 (C16:1ω7c and/or C16:1 ω6c). The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and two unidentified lipids. The draft genome size was 5,115,008 bp with DNA G + C content of 54.3%. Based on phylogenetic analyses and whole genomic comparisons, strain SM2-42 T represented a novel species, for which the name Oceanobacter mangrovi sp. nov. was proposed. The type strain was SM2-42 T (= MCCC 1K06300T = KCTC 82938 T).

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Data Availability

The GenBank/EMBL/DDBJ accession numbers of the 16S rRNA gene sequence and the draft genome sequence of strain SM2-42 T are MT829535 and JAHXZR000000000, respectively.

Abbreviations

MCCC:

Marine culture collection of China

KCTC:

Korean collection for type cultures

ANI:

Average nucleotide identity

dDDH:

Digital DNA-DNA hybridization

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Funding

This work was supported by the project of Natural Science Foundation of Fujian Province (2020J01789) and National Science Foundation of China (41706139).

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Authors and Affiliations

  1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China

    Zhaobin Huang, Yuanyuan Huang & Xiaozhou Huang

  2. Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China

    Zhaobin Huang & Xiaozhou Huang

  3. Key Laboratory of Marine Genetic Resources, Ministry of Natural Resources, Third Institute of Oceanography, Xiamen, China

    Qiliang Lai & Chunming Dong

  4. School of Life Sciences, Xiamen University, Xiamen, China

    Xinlan Chen

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  1. Zhaobin Huang
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  4. Xinlan Chen
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  6. Xiaozhou Huang
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Contributions

ZH conceived the study and drafted the manuscript. ZH, YH, QL, XC, CD and XH performed the experiment and analyze the data.

Corresponding author

Correspondence to Zhaobin Huang.

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Huang, Z., Huang, Y., Lai, Q. et al. Oceanobacter mangrovi Sp. Nov., a Novel Poly-β-hydroxybutyrate Accumulating Bacterium Isolated from Mangrove Sediment. Curr Microbiol 79, 100 (2022). https://doi.org/10.1007/s00284-022-02798-5

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