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Untargeted Phylogenetic Group III of Multi-drug-Resistant Bacillus cereus Isolated Using Fraser Medium from Retail Chickens in Ho Chi Minh City

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Abstract

The prevalence of food-borne bacteria in developing countries is less well understood than in developed countries. The ISO11290-1 isolation method is commonly used to study Listeria contamination in chicken; however, all isolates are identified as untargeted Bacillus cereus. This study aimed to determine the classification, antibiotic susceptibility, and virulence genes of B. cereus isolated from retail chickens in Vietnam. Bacterial isolation using the ISO11290-1 method yielded 12 strains of B. cereus from seven out of 60 chickens. For determining bacterial diversity, panC and multilocus sequence typing (MLST) analyses were performed. PanC analysis showed that all seven strains belong to the phylogenetic group III, to which the highest risk of foodborne illnesses was associated. MLST analysis showed that most strains contained a ST205 complex; further, all strains were found to be resistant to ampicillin, ciprofloxacin, and tetracycline. Virulence genes were also investigated. ces, a cereulide-related gene, was detected in 50% of the isolated strains, followed by cytK, nheA, and hblA enterotoxins in 41.7%, 16.7%, and 25% of the strains, respectively. In conclusion, B. cereus may be erroneously detected when attempting to detect Listeria in food using the ISO11290-1 method. Further study of the prevalence of B. cereus in Vietnamese food is needed to improve food safety.

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Acknowledgements

This work was supported by the MEXT/JSPS KAKENHI Grant Number 18KK0168.

Funding

This work was supported by JSPS KAKENHI Grant Number 18KK0168.

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Authors and Affiliations

  1. Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan

    Tatsuya Nakayama

  2. Division of Biomedical Food Research, National Institute of Health Sciences, Kawasaki, Kanagawa, Japan

    Tatsuya Nakayama & Shiori Yamamoto

  3. Department of Microbiology, Osaka Institute of Public Health, Higashinari-ku, Osaka, Japan

    Takahiro Yamaguchi

  4. Department of Microbiology, Kanagawa Prefecture Institute of Public Health, Chigasaki, Kanagawa, Japan

    Michio Jinnai

  5. Ho Chi Minh Institute of Public Health, Ho Chi Minh City, Vietnam

    Hien Thi Li, Phong Thanh Ngo, Doan Nguyen Minh Tran, Oanh Thi Hoang Nguyen, Phuong Hoai Hoang, Phuc Do Nguyen & Chinh Van Dang

  6. Center for Microorganism Control, Osaka Prefecture University, Sakai, Osaka, Japan

    Yuko Kumeda

  7. Faculty of Contemporary Human Life Science, Tezukayama University, Nara, Japan

    Atsushi Hase

Authors
  1. Tatsuya Nakayama
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  2. Takahiro Yamaguchi
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  3. Michio Jinnai
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  4. Shiori Yamamoto
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  7. Doan Nguyen Minh Tran
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  8. Oanh Thi Hoang Nguyen
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  9. Phuong Hoai Hoang
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  11. Chinh Van Dang
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  13. Atsushi Hase
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Contributions

TN, YK and AH conceived the study. TN, TY, MJ, PTN and AH purchased chicken meat from markets. TN, TY, MJ, SY, HTL, PTN, DNMT, OTHN and PHH performed experiment. TN, TY, MJ, PDN and CVD analysed the data. TN, TY and MJ wrote the manuscript. TN, TY and MJ revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Tatsuya Nakayama.

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Nakayama, T., Yamaguchi, T., Jinnai, M. et al. Untargeted Phylogenetic Group III of Multi-drug-Resistant Bacillus cereus Isolated Using Fraser Medium from Retail Chickens in Ho Chi Minh City. Curr Microbiol 78, 3115–3123 (2021). https://doi.org/10.1007/s00284-021-02562-1

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  • DOI: https://doi.org/10.1007/s00284-021-02562-1

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